PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.
Guardado en:
Autores principales: | , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/f79a51c5dc574d0197305537b3b9d5a5 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:f79a51c5dc574d0197305537b3b9d5a5 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:f79a51c5dc574d0197305537b3b9d5a52021-12-02T14:03:44ZPANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens10.1038/s42003-021-01751-92399-3642https://doaj.org/article/f79a51c5dc574d0197305537b3b9d5a52021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01751-9https://doaj.org/toc/2399-3642Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.Iain J. MacLeodChristopher F. RowleyM. EssexNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Biology (General) QH301-705.5 |
spellingShingle |
Biology (General) QH301-705.5 Iain J. MacLeod Christopher F. Rowley M. Essex PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
description |
Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development. |
format |
article |
author |
Iain J. MacLeod Christopher F. Rowley M. Essex |
author_facet |
Iain J. MacLeod Christopher F. Rowley M. Essex |
author_sort |
Iain J. MacLeod |
title |
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
title_short |
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
title_full |
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
title_fullStr |
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
title_full_unstemmed |
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
title_sort |
pandaa intentionally violates conventional qpcr design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/f79a51c5dc574d0197305537b3b9d5a5 |
work_keys_str_mv |
AT iainjmacleod pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens AT christopherfrowley pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens AT messex pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens |
_version_ |
1718392118210199552 |