PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.

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Auteurs principaux:	Iain J. MacLeod, Christopher F. Rowley, M. Essex
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/f79a51c5dc574d0197305537b3b9d5a5
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spelling	oai:doaj.org-article:f79a51c5dc574d0197305537b3b9d5a52021-12-02T14:03:44ZPANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens10.1038/s42003-021-01751-92399-3642https://doaj.org/article/f79a51c5dc574d0197305537b3b9d5a52021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01751-9https://doaj.org/toc/2399-3642Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.Iain J. MacLeodChristopher F. RowleyM. EssexNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Iain J. MacLeod Christopher F. Rowley M. Essex PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
description	Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.
format	article
author	Iain J. MacLeod Christopher F. Rowley M. Essex
author_facet	Iain J. MacLeod Christopher F. Rowley M. Essex
author_sort	Iain J. MacLeod
title	PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_short	PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_full	PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_fullStr	PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_full_unstemmed	PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_sort	pandaa intentionally violates conventional qpcr design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f79a51c5dc574d0197305537b3b9d5a5
work_keys_str_mv	AT iainjmacleod pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens AT christopherfrowley pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens AT messex pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens
_version_	1718392118210199552

PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

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