Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.

<h4>Background aims</h4>Maternal undernutrition programs metabolic adaptations which are ultimately detrimental to adult. L-tryptophan supplementation was given to manipulate the long-term sequelae of early-life programming by undernutrition and explore whether cultured cells retain circ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elizabeth Nascimento, Omar Guzman-Quevedo, Nellie Delacourt, Raquel da Silva Aragão, Georgina Perez-Garcia, Sandra Lopes de Souza, Raul Manhães-de-Castro, Francisco Bolaños-Jiménez, Bertrand Kaeffer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f79e4b3ca78d4a4aaa24cbc6a272445b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f79e4b3ca78d4a4aaa24cbc6a272445b
record_format	dspace
spelling	oai:doaj.org-article:f79e4b3ca78d4a4aaa24cbc6a272445b2021-11-18T07:55:54ZLong-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.1932-620310.1371/journal.pone.0056231https://doaj.org/article/f79e4b3ca78d4a4aaa24cbc6a272445b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23460795/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background aims</h4>Maternal undernutrition programs metabolic adaptations which are ultimately detrimental to adult. L-tryptophan supplementation was given to manipulate the long-term sequelae of early-life programming by undernutrition and explore whether cultured cells retain circadian clock dysregulation.<h4>Methods</h4>Male rat pups from mothers fed on low protein (8%, LP) or control (18%, CP) diet were given, one hour before light off, an oral bolus of L-tryptophan (125 mg/kg) between Day-12 and Day-21 of age. Body weight, food intake, blood glucose along with the capacity of colonization of primary cells from biopsies were measured during the young (45-55 days) and adult (110-130 days) phases. Circadian clock oscillations were re-induced by a serum shock over 30 hours on near-confluent cell monolayers to follow PERIOD1 and CLOCK proteins by Fluorescent Linked ImmunoSorbent Assay (FLISA) and period1 and bmal1 mRNA by RT-PCR. Cell survival in amino acid-free conditions were used to measure circadian expression of MAP-LC3B, MAP-LC3B-FP and Survivin.<h4>Results</h4>Tryptophan supplementation did not alter body weight gain nor feeding pattern. By three-way ANOVA of blood glucose, sampling time was found significant during all phases. A significant interaction between daily bolus (Tryptophan, saline) and diets (LP, CP) were found during young (p = 0.0291) and adult (p = 0.0285) phases. In adult phase, the capacity of colonization at seeding of primary cells was twice lower for LP rats. By three-way ANOVA of PERIOD1 perinuclear/nuclear immunoreactivity during young phase, we found a significant effect of diets (p = 0.049), daily bolus (p<0.0001) and synchronizer hours (p = 0.0002). All factors were significantly interacting (p = 0.0148). MAP-LC3B, MAP-LC3B-FP and Survivin were altered according to diets in young phase.<h4>Conclusions</h4>Sequelae of early-life undernutrition and the effects of L-tryptophan supplementation can be monitored non-invasively by circadian sampling of blood D-glucose and on the expression of PERIOD1 protein in established primary cell lines.Elizabeth NascimentoOmar Guzman-QuevedoNellie DelacourtRaquel da Silva AragãoGeorgina Perez-GarciaSandra Lopes de SouzaRaul Manhães-de-CastroFrancisco Bolaños-JiménezBertrand KaefferPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56231 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elizabeth Nascimento Omar Guzman-Quevedo Nellie Delacourt Raquel da Silva Aragão Georgina Perez-Garcia Sandra Lopes de Souza Raul Manhães-de-Castro Francisco Bolaños-Jiménez Bertrand Kaeffer Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
description	<h4>Background aims</h4>Maternal undernutrition programs metabolic adaptations which are ultimately detrimental to adult. L-tryptophan supplementation was given to manipulate the long-term sequelae of early-life programming by undernutrition and explore whether cultured cells retain circadian clock dysregulation.<h4>Methods</h4>Male rat pups from mothers fed on low protein (8%, LP) or control (18%, CP) diet were given, one hour before light off, an oral bolus of L-tryptophan (125 mg/kg) between Day-12 and Day-21 of age. Body weight, food intake, blood glucose along with the capacity of colonization of primary cells from biopsies were measured during the young (45-55 days) and adult (110-130 days) phases. Circadian clock oscillations were re-induced by a serum shock over 30 hours on near-confluent cell monolayers to follow PERIOD1 and CLOCK proteins by Fluorescent Linked ImmunoSorbent Assay (FLISA) and period1 and bmal1 mRNA by RT-PCR. Cell survival in amino acid-free conditions were used to measure circadian expression of MAP-LC3B, MAP-LC3B-FP and Survivin.<h4>Results</h4>Tryptophan supplementation did not alter body weight gain nor feeding pattern. By three-way ANOVA of blood glucose, sampling time was found significant during all phases. A significant interaction between daily bolus (Tryptophan, saline) and diets (LP, CP) were found during young (p = 0.0291) and adult (p = 0.0285) phases. In adult phase, the capacity of colonization at seeding of primary cells was twice lower for LP rats. By three-way ANOVA of PERIOD1 perinuclear/nuclear immunoreactivity during young phase, we found a significant effect of diets (p = 0.049), daily bolus (p<0.0001) and synchronizer hours (p = 0.0002). All factors were significantly interacting (p = 0.0148). MAP-LC3B, MAP-LC3B-FP and Survivin were altered according to diets in young phase.<h4>Conclusions</h4>Sequelae of early-life undernutrition and the effects of L-tryptophan supplementation can be monitored non-invasively by circadian sampling of blood D-glucose and on the expression of PERIOD1 protein in established primary cell lines.
format	article
author	Elizabeth Nascimento Omar Guzman-Quevedo Nellie Delacourt Raquel da Silva Aragão Georgina Perez-Garcia Sandra Lopes de Souza Raul Manhães-de-Castro Francisco Bolaños-Jiménez Bertrand Kaeffer
author_facet	Elizabeth Nascimento Omar Guzman-Quevedo Nellie Delacourt Raquel da Silva Aragão Georgina Perez-Garcia Sandra Lopes de Souza Raul Manhães-de-Castro Francisco Bolaños-Jiménez Bertrand Kaeffer
author_sort	Elizabeth Nascimento
title	Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
title_short	Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
title_full	Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
title_fullStr	Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
title_full_unstemmed	Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
title_sort	long-lasting effect of perinatal exposure to l-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f79e4b3ca78d4a4aaa24cbc6a272445b
work_keys_str_mv	AT elizabethnascimento longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT omarguzmanquevedo longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT nelliedelacourt longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT raqueldasilvaaragao longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT georginaperezgarcia longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT sandralopesdesouza longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT raulmanhaesdecastro longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT franciscobolanosjimenez longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction AT bertrandkaeffer longlastingeffectofperinatalexposuretoltryptophanoncircadianclockofprimarycelllinesestablishedfrommaleoffspringbornfrommothersfedondietaryproteinrestriction
_version_	1718422768340434944

Long-lasting effect of perinatal exposure to L-tryptophan on circadian clock of primary cell lines established from male offspring born from mothers fed on dietary protein restriction.

Ejemplares similares