Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines

Abstract Colorectal cancer (CRC) is the fourth most lethal disease worldwide. Despite an urgent need for therapeutic advance, selective target identification in a preclinical phase is hampered by molecular and metabolic variations between cellular models. To foster optimal model selection from a tra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Caroline Rombouts, Margot De Spiegeleer, Lieven Van Meulebroek, Lynn Vanhaecke, Winnok H. De Vos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7a186251aaa46ceaae9ba3bc0578cf8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f7a186251aaa46ceaae9ba3bc0578cf8
record_format	dspace
spelling	oai:doaj.org-article:f7a186251aaa46ceaae9ba3bc0578cf82021-12-02T16:35:05ZComprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines10.1038/s41598-021-96252-42045-2322https://doaj.org/article/f7a186251aaa46ceaae9ba3bc0578cf82021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96252-4https://doaj.org/toc/2045-2322Abstract Colorectal cancer (CRC) is the fourth most lethal disease worldwide. Despite an urgent need for therapeutic advance, selective target identification in a preclinical phase is hampered by molecular and metabolic variations between cellular models. To foster optimal model selection from a translational perspective, we performed untargeted ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry-based polar metabolomics and lipidomics to non-transformed (CCD841-CON and FHC) and transformed (HCT116, HT29, Caco2, SW480 and SW948) colon cell lines as well as tissue samples from ten colorectal cancer patients. This unveiled metabolic signatures discriminating the transformed from the non-transformed state. Metabolites involved in glutaminolysis, tryptophan catabolism, pyrimidine, lipid and carnitine synthesis were elevated in transformed cells and cancerous tissue, whereas those involved in the glycerol-3-phosphate shuttle, urea cycle and redox reactions were lowered. The degree of glutaminolysis and lipid synthesis was specific to the colon cancer cell line at hand. Thus, our study exposed pathways that are specifically associated with the transformation state and revealed differences between colon cancer cell lines that should be considered when targeting cancer-associated pathways.Caroline RomboutsMargot De SpiegeleerLieven Van MeulebroekLynn VanhaeckeWinnok H. De VosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Caroline Rombouts Margot De Spiegeleer Lieven Van Meulebroek Lynn Vanhaecke Winnok H. De Vos Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
description	Abstract Colorectal cancer (CRC) is the fourth most lethal disease worldwide. Despite an urgent need for therapeutic advance, selective target identification in a preclinical phase is hampered by molecular and metabolic variations between cellular models. To foster optimal model selection from a translational perspective, we performed untargeted ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry-based polar metabolomics and lipidomics to non-transformed (CCD841-CON and FHC) and transformed (HCT116, HT29, Caco2, SW480 and SW948) colon cell lines as well as tissue samples from ten colorectal cancer patients. This unveiled metabolic signatures discriminating the transformed from the non-transformed state. Metabolites involved in glutaminolysis, tryptophan catabolism, pyrimidine, lipid and carnitine synthesis were elevated in transformed cells and cancerous tissue, whereas those involved in the glycerol-3-phosphate shuttle, urea cycle and redox reactions were lowered. The degree of glutaminolysis and lipid synthesis was specific to the colon cancer cell line at hand. Thus, our study exposed pathways that are specifically associated with the transformation state and revealed differences between colon cancer cell lines that should be considered when targeting cancer-associated pathways.
format	article
author	Caroline Rombouts Margot De Spiegeleer Lieven Van Meulebroek Lynn Vanhaecke Winnok H. De Vos
author_facet	Caroline Rombouts Margot De Spiegeleer Lieven Van Meulebroek Lynn Vanhaecke Winnok H. De Vos
author_sort	Caroline Rombouts
title	Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
title_short	Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
title_full	Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
title_fullStr	Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
title_full_unstemmed	Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
title_sort	comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f7a186251aaa46ceaae9ba3bc0578cf8
work_keys_str_mv	AT carolinerombouts comprehensivepolarmetabolomicsandlipidomicsprofilingdiscriminatesthetransformedfromthenontransformedstateincolontissueandcelllines AT margotdespiegeleer comprehensivepolarmetabolomicsandlipidomicsprofilingdiscriminatesthetransformedfromthenontransformedstateincolontissueandcelllines AT lievenvanmeulebroek comprehensivepolarmetabolomicsandlipidomicsprofilingdiscriminatesthetransformedfromthenontransformedstateincolontissueandcelllines AT lynnvanhaecke comprehensivepolarmetabolomicsandlipidomicsprofilingdiscriminatesthetransformedfromthenontransformedstateincolontissueandcelllines AT winnokhdevos comprehensivepolarmetabolomicsandlipidomicsprofilingdiscriminatesthetransformedfromthenontransformedstateincolontissueandcelllines
_version_	1718383789441286144

Comprehensive polar metabolomics and lipidomics profiling discriminates the transformed from the non-transformed state in colon tissue and cell lines

Ejemplares similares