Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia

Abstract. Hasanah R, Saepuloh U, Perwitasari-Farajallah D, Sinaga W, Hastuti Y, Sajuthi D. 2020. Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia. Biodiversitas 21: 2854-2861. Hylobates klossii is an endemic...

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Autores principales:	Rizka Hasanah, UUS SAEPULOH, DYAH PERWITASARI-FARAJALLAH, WALBERTO SINAGA, YOHANA TRI HASTUTI, DONDIN SAJUTHI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	d-loop hybrid h. klossii i-tasser tspy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f7a2c232464848e79fab169430835f52
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spelling	oai:doaj.org-article:f7a2c232464848e79fab169430835f522021-11-22T00:40:46ZGenetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia1412-033X2085-472210.13057/biodiv/d210664https://doaj.org/article/f7a2c232464848e79fab169430835f522020-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5058https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Hasanah R, Saepuloh U, Perwitasari-Farajallah D, Sinaga W, Hastuti Y, Sajuthi D. 2020. Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia. Biodiversitas 21: 2854-2861. Hylobates klossii is an endemic primate on the Mentawai Island, Indonesia that categorized as endangered species due to fragmentation, illegal hunting, and habitat loss. This study aimed to analyze the genetic characterization of H. klossii based on maternal and paternal lineages. The samples were collected from TSI (Bogor, Indonesia) cage consist of two males and two females (blood and fecal of four individuals) and Siberut Island, Mentawai, West Sumatra, Indonesia from one unknown gender (one individual fecal sample). PCR amplification was carried out on DNA and cDNA samples using specific primers for D-loop MtDNA and TSPY genes. The amplicon was analyzed with MEGA-7. The dimensional structure model of the TSPY protein was determined using I-TASSER and visualized with PyMol. The maternal analysis showed five samples consisted of four haplotypes revealed that Nam-nam and April were identic with 0% genetic distance. It’s revealed that Nam-nam and April were mother and son. The paternal analysis of two H. klossii males revealed that April was the closest relative to H. muelleri (99.56% identity) and Gou-gou to H. klossii (100% identity). Characterization of partial TSPY gene (990 bp) of April and Gou-Gou indicated four amino acids mutation, causing the shift in-amino acid structure position. The results assumed that April was a cross-breeding between H. klossii female and H. muelleri male. This genetic characterization is expected to support the conservation efforts through recommendations for good management in H. klossii preservation.Rizka HasanahUUS SAEPULOHDYAH PERWITASARI-FARAJALLAHWALBERTO SINAGAYOHANA TRI HASTUTIDONDIN SAJUTHIMBI & UNS Soloarticled-loophybridh. klossiii-tassertspyBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	d-loop hybrid h. klossii i-tasser tspy Biology (General) QH301-705.5
spellingShingle	d-loop hybrid h. klossii i-tasser tspy Biology (General) QH301-705.5 Rizka Hasanah UUS SAEPULOH DYAH PERWITASARI-FARAJALLAH WALBERTO SINAGA YOHANA TRI HASTUTI DONDIN SAJUTHI Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia
description	Abstract. Hasanah R, Saepuloh U, Perwitasari-Farajallah D, Sinaga W, Hastuti Y, Sajuthi D. 2020. Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia. Biodiversitas 21: 2854-2861. Hylobates klossii is an endemic primate on the Mentawai Island, Indonesia that categorized as endangered species due to fragmentation, illegal hunting, and habitat loss. This study aimed to analyze the genetic characterization of H. klossii based on maternal and paternal lineages. The samples were collected from TSI (Bogor, Indonesia) cage consist of two males and two females (blood and fecal of four individuals) and Siberut Island, Mentawai, West Sumatra, Indonesia from one unknown gender (one individual fecal sample). PCR amplification was carried out on DNA and cDNA samples using specific primers for D-loop MtDNA and TSPY genes. The amplicon was analyzed with MEGA-7. The dimensional structure model of the TSPY protein was determined using I-TASSER and visualized with PyMol. The maternal analysis showed five samples consisted of four haplotypes revealed that Nam-nam and April were identic with 0% genetic distance. It’s revealed that Nam-nam and April were mother and son. The paternal analysis of two H. klossii males revealed that April was the closest relative to H. muelleri (99.56% identity) and Gou-gou to H. klossii (100% identity). Characterization of partial TSPY gene (990 bp) of April and Gou-Gou indicated four amino acids mutation, causing the shift in-amino acid structure position. The results assumed that April was a cross-breeding between H. klossii female and H. muelleri male. This genetic characterization is expected to support the conservation efforts through recommendations for good management in H. klossii preservation.
format	article
author	Rizka Hasanah UUS SAEPULOH DYAH PERWITASARI-FARAJALLAH WALBERTO SINAGA YOHANA TRI HASTUTI DONDIN SAJUTHI
author_facet	Rizka Hasanah UUS SAEPULOH DYAH PERWITASARI-FARAJALLAH WALBERTO SINAGA YOHANA TRI HASTUTI DONDIN SAJUTHI
author_sort	Rizka Hasanah
title	Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia
title_short	Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia
title_full	Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia
title_fullStr	Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia
title_full_unstemmed	Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia
title_sort	genetic characterization based on the d-loop mtdna and the tspy genes of the dwarf siamang (hylobates klossii) of mentawai island, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/f7a2c232464848e79fab169430835f52
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Genetic characterization based on the D-loop MtDNA and the TSPY genes of the Dwarf Siamang (Hylobates klossii) of Mentawai Island, Indonesia

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