Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance

MicroRNAs are a class of small regulatory RNAs in plants, which play vital roles during various abiotic and abiotic stress conditions including plant processes. In this present study, we examined the expression of miRNAs and their predicted target expression levels during heat stress in banana. Out...

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Autores principales:	S M Vidya, K V Ravishankar, R H Laxman
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2018
Materias:	acquired thermo tolerance, banana, heat stress, mirna,thermo protective genes. Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/f7a54375c0324229b6d98a14d8d80976
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spelling	oai:doaj.org-article:f7a54375c0324229b6d98a14d8d809762021-12-02T08:27:33ZGenome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance0973-354X2582-4899https://doaj.org/article/f7a54375c0324229b6d98a14d8d809762018-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/35https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899MicroRNAs are a class of small regulatory RNAs in plants, which play vital roles during various abiotic and abiotic stress conditions including plant processes. In this present study, we examined the expression of miRNAs and their predicted target expression levels during heat stress in banana. Out of 235 miRNA found in Musa, 40 miRNA showed homology to heat responsive miRNAs from other plants. Further, 14 targets for miRNA were predicted that are potentially regulated by their cognate miRNAs and were monitored under three stages of stress viz, induction, induction + lethal alone using qPCR analysis. The results suggest that generally, there is a negative relationship in the expression patterns of miRNA and their predicted cognate targets - HSP70, HSP90, SAP, DNAj genes. These were highly up regulated and their respective miRNAs showed lower expression. This is the first report in banana, which demonstrated that during induction stress, various thermo-protective genes are activated at initial stages of stress to achieve thermotolerance through altered miRNA expression. The results will help in broadening our understanding acquired thermotolerance and their regulation by miRNAs in plants.S M VidyaK V RavishankarR H LaxmanSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleacquired thermo tolerance, banana, heat stress, mirna,thermo protective genes.Plant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 13, Iss 1, Pp 61-71 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	acquired thermo tolerance, banana, heat stress, mirna,thermo protective genes. Plant culture SB1-1110
spellingShingle	acquired thermo tolerance, banana, heat stress, mirna,thermo protective genes. Plant culture SB1-1110 S M Vidya K V Ravishankar R H Laxman Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
description	MicroRNAs are a class of small regulatory RNAs in plants, which play vital roles during various abiotic and abiotic stress conditions including plant processes. In this present study, we examined the expression of miRNAs and their predicted target expression levels during heat stress in banana. Out of 235 miRNA found in Musa, 40 miRNA showed homology to heat responsive miRNAs from other plants. Further, 14 targets for miRNA were predicted that are potentially regulated by their cognate miRNAs and were monitored under three stages of stress viz, induction, induction + lethal alone using qPCR analysis. The results suggest that generally, there is a negative relationship in the expression patterns of miRNA and their predicted cognate targets - HSP70, HSP90, SAP, DNAj genes. These were highly up regulated and their respective miRNAs showed lower expression. This is the first report in banana, which demonstrated that during induction stress, various thermo-protective genes are activated at initial stages of stress to achieve thermotolerance through altered miRNA expression. The results will help in broadening our understanding acquired thermotolerance and their regulation by miRNAs in plants.
format	article
author	S M Vidya K V Ravishankar R H Laxman
author_facet	S M Vidya K V Ravishankar R H Laxman
author_sort	S M Vidya
title	Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
title_short	Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
title_full	Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
title_fullStr	Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
title_full_unstemmed	Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
title_sort	genome wide analysis of heat responsive micrornas in banana during acquired thermo tolerance
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2018
url	https://doaj.org/article/f7a54375c0324229b6d98a14d8d80976
work_keys_str_mv	AT smvidya genomewideanalysisofheatresponsivemicrornasinbananaduringacquiredthermotolerance AT kvravishankar genomewideanalysisofheatresponsivemicrornasinbananaduringacquiredthermotolerance AT rhlaxman genomewideanalysisofheatresponsivemicrornasinbananaduringacquiredthermotolerance
_version_	1718398519805476864

Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance

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