An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model

Abstract The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in pr...

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Autores principales:	Jasdave S. Chahal, Tao Fang, Andrew W. Woodham, Omar F. Khan, Jingjing Ling, Daniel G. Anderson, Hidde L. Ploegh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7a59228dbcc45f49fb90892d8c11545
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spelling	oai:doaj.org-article:f7a59228dbcc45f49fb90892d8c115452021-12-02T15:05:03ZAn RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model10.1038/s41598-017-00193-w2045-2322https://doaj.org/article/f7a59228dbcc45f49fb90892d8c115452017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00193-whttps://doaj.org/toc/2045-2322Abstract The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/6 mice as assayed by ELISA. Using this tool, we identified a unique H-2Db-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus.Jasdave S. ChahalTao FangAndrew W. WoodhamOmar F. KhanJingjing LingDaniel G. AndersonHidde L. PloeghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jasdave S. Chahal Tao Fang Andrew W. Woodham Omar F. Khan Jingjing Ling Daniel G. Anderson Hidde L. Ploegh An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
description	Abstract The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV’s neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particularly T cell responses, remain sparse. We deployed RNA nanoparticle technology to create a vaccine candidate that elicited ZIKV E protein-specific IgG responses in C57BL/6 mice as assayed by ELISA. Using this tool, we identified a unique H-2Db-restricted epitope to which there was a CD8+ T cell response in mice immunized with our modified dendrimer-based RNA nanoparticle vaccine. These results demonstrate that this approach can be used to evaluate new candidate antigens and identify immune correlates without the use of live virus.
format	article
author	Jasdave S. Chahal Tao Fang Andrew W. Woodham Omar F. Khan Jingjing Ling Daniel G. Anderson Hidde L. Ploegh
author_facet	Jasdave S. Chahal Tao Fang Andrew W. Woodham Omar F. Khan Jingjing Ling Daniel G. Anderson Hidde L. Ploegh
author_sort	Jasdave S. Chahal
title	An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
title_short	An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
title_full	An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
title_fullStr	An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
title_full_unstemmed	An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model
title_sort	rna nanoparticle vaccine against zika virus elicits antibody and cd8+ t cell responses in a mouse model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f7a59228dbcc45f49fb90892d8c11545
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An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model

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