Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.

<h4>Background and purpose</h4>To investigate the effect of locally applied nimodipine prolonged-release microparticles on angiographic vasospasm and secondary brain injury after experimental subarachnoid hemorrhage (SAH).<h4>Methods</h4>70 male Wistar rats were categorized i...

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Autores principales:	Daniel Hänggi, Jason Perrin, Sven Eicker, Kerim Beseoglu, Nima Etminan, Marcel Alexander Kamp, Hi-Jae Heiroth, Nadia Bege, Stephan Macht, Katrin Frauenknecht, Clemens Sommer, Thomas Kissel, Hans-Jakob Steiger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7a63de9084141b09ad1a8690d99bbde
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spelling	oai:doaj.org-article:f7a63de9084141b09ad1a8690d99bbde2021-11-18T08:14:16ZLocal delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.1932-620310.1371/journal.pone.0042597https://doaj.org/article/f7a63de9084141b09ad1a8690d99bbde2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049732/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and purpose</h4>To investigate the effect of locally applied nimodipine prolonged-release microparticles on angiographic vasospasm and secondary brain injury after experimental subarachnoid hemorrhage (SAH).<h4>Methods</h4>70 male Wistar rats were categorized into three groups: 1) sham operated animals (control), 2) animals with SAH only (control) and the 3) treatment group. SAH was induced using the double hemorrhage model. The treatment group received different concentrations (20%, 30% or 40%) of nimodipine microparticles. Angiographic vasospasm was assessed 5 days later using digital subtraction angiography (DSA). Histological analysis of frozen sections was performed using H&E-staining as well as Iba1 and MAP2 immunohistochemistry.<h4>Results</h4>DSA images were sufficient for assessment in 42 animals. Severe angiographic vasospasm was present in group 2 (SAH only), as compared to the sham operated group (p<0.001). Only animals within group 3 and the highest nimodipine microparticles concentration (40%) as well as group 1 (sham) demonstrated the largest intracranial artery diameters. Variation in vessel calibers, however, did not result in differences in Iba-1 or MAP2 expression, i.e. in histological findings for secondary brain injury.<h4>Conclusions</h4>Local delivery of high-dose nimodipine prolonged-release microparticles at high concentration resulted in significant reduction in angiographic vasospasm after experimental SAH and with no histological signs for matrix toxicity.Daniel HänggiJason PerrinSven EickerKerim BeseogluNima EtminanMarcel Alexander KampHi-Jae HeirothNadia BegeStephan MachtKatrin FrauenknechtClemens SommerThomas KisselHans-Jakob SteigerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e42597 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Hänggi Jason Perrin Sven Eicker Kerim Beseoglu Nima Etminan Marcel Alexander Kamp Hi-Jae Heiroth Nadia Bege Stephan Macht Katrin Frauenknecht Clemens Sommer Thomas Kissel Hans-Jakob Steiger Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
description	<h4>Background and purpose</h4>To investigate the effect of locally applied nimodipine prolonged-release microparticles on angiographic vasospasm and secondary brain injury after experimental subarachnoid hemorrhage (SAH).<h4>Methods</h4>70 male Wistar rats were categorized into three groups: 1) sham operated animals (control), 2) animals with SAH only (control) and the 3) treatment group. SAH was induced using the double hemorrhage model. The treatment group received different concentrations (20%, 30% or 40%) of nimodipine microparticles. Angiographic vasospasm was assessed 5 days later using digital subtraction angiography (DSA). Histological analysis of frozen sections was performed using H&E-staining as well as Iba1 and MAP2 immunohistochemistry.<h4>Results</h4>DSA images were sufficient for assessment in 42 animals. Severe angiographic vasospasm was present in group 2 (SAH only), as compared to the sham operated group (p<0.001). Only animals within group 3 and the highest nimodipine microparticles concentration (40%) as well as group 1 (sham) demonstrated the largest intracranial artery diameters. Variation in vessel calibers, however, did not result in differences in Iba-1 or MAP2 expression, i.e. in histological findings for secondary brain injury.<h4>Conclusions</h4>Local delivery of high-dose nimodipine prolonged-release microparticles at high concentration resulted in significant reduction in angiographic vasospasm after experimental SAH and with no histological signs for matrix toxicity.
format	article
author	Daniel Hänggi Jason Perrin Sven Eicker Kerim Beseoglu Nima Etminan Marcel Alexander Kamp Hi-Jae Heiroth Nadia Bege Stephan Macht Katrin Frauenknecht Clemens Sommer Thomas Kissel Hans-Jakob Steiger
author_facet	Daniel Hänggi Jason Perrin Sven Eicker Kerim Beseoglu Nima Etminan Marcel Alexander Kamp Hi-Jae Heiroth Nadia Bege Stephan Macht Katrin Frauenknecht Clemens Sommer Thomas Kissel Hans-Jakob Steiger
author_sort	Daniel Hänggi
title	Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
title_short	Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
title_full	Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
title_fullStr	Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
title_full_unstemmed	Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
title_sort	local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f7a63de9084141b09ad1a8690d99bbde
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Local delivery of nimodipine by prolonged-release microparticles-feasibility, effectiveness and dose-finding in experimental subarachnoid hemorrhage.

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