Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation

Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vučićević Ivana, Marinković Darko, Kukolj Vladimir, Vučićević Miloš, Mirilović Milorad, Nešić Slađan, Aleksić-Kovačević Sanja
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	dogs mast cell tumor morphology classification cd117 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/f7afe47e0ba34de38da6b20bf634faff
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f7afe47e0ba34de38da6b20bf634faff
record_format	dspace
spelling	oai:doaj.org-article:f7afe47e0ba34de38da6b20bf634faff2021-11-17T21:27:51ZKit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation1820-744810.1515/acve-2016-0019https://doaj.org/article/f7afe47e0ba34de38da6b20bf634faff2016-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0019https://doaj.org/toc/1820-7448Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation.Vučićević IvanaMarinković DarkoKukolj VladimirVučićević MilošMirilović MiloradNešić SlađanAleksić-Kovačević SanjaSciendoarticledogsmast cell tumormorphologyclassificationcd117Veterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 2, Pp 222-233 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	dogs mast cell tumor morphology classification cd117 Veterinary medicine SF600-1100
spellingShingle	dogs mast cell tumor morphology classification cd117 Veterinary medicine SF600-1100 Vučićević Ivana Marinković Darko Kukolj Vladimir Vučićević Miloš Mirilović Milorad Nešić Slađan Aleksić-Kovačević Sanja Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation
description	Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation.
format	article
author	Vučićević Ivana Marinković Darko Kukolj Vladimir Vučićević Miloš Mirilović Milorad Nešić Slađan Aleksić-Kovačević Sanja
author_facet	Vučićević Ivana Marinković Darko Kukolj Vladimir Vučićević Miloš Mirilović Milorad Nešić Slađan Aleksić-Kovačević Sanja
author_sort	Vučićević Ivana
title	Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation
title_short	Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation
title_full	Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation
title_fullStr	Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation
title_full_unstemmed	Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation
title_sort	kit receptor expression in canine cutaneous mast cell tumors (cmcts) without c-kit mutation
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/f7afe47e0ba34de38da6b20bf634faff
work_keys_str_mv	AT vucicevicivana kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation AT marinkovicdarko kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation AT kukoljvladimir kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation AT vucicevicmilos kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation AT mirilovicmilorad kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation AT nesicslađan kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation AT aleksickovacevicsanja kitreceptorexpressionincaninecutaneousmastcelltumorscmctswithoutckitmutation
_version_	1718425328299278336

Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation

Ejemplares similares