Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.

<h4>Background</h4>Mechanical overload leads to cardiac hypertrophy and mechanical unloading to cardiac atrophy. Both conditions produce similar transcriptional changes including a re-expression of fetal genes, despite obvious differences in phenotype. MicroRNAs (miRNAs) are discussed as...

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Autores principales:	Ali El-Armouche, Alexander Peter Schwoerer, Christiane Neuber, Julius Emons, Daniel Biermann, Thomas Christalla, Adam Grundhoff, Thomas Eschenhagen, Wolfram Hubertus Zimmermann, Heimo Ehmke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7b34f21c2c045f49d98f65bbcbf7772
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spelling	oai:doaj.org-article:f7b34f21c2c045f49d98f65bbcbf77722021-11-18T07:01:52ZCommon microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.1932-620310.1371/journal.pone.0014263https://doaj.org/article/f7b34f21c2c045f49d98f65bbcbf77722010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21151612/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mechanical overload leads to cardiac hypertrophy and mechanical unloading to cardiac atrophy. Both conditions produce similar transcriptional changes including a re-expression of fetal genes, despite obvious differences in phenotype. MicroRNAs (miRNAs) are discussed as superordinate regulators of global gene networks acting mainly at the translational level. Here, we hypothesized that defined sets of miRNAs may determine the direction of cardiomyocyte plasticity responses.<h4>Methodology/principal findings</h4>We employed ascending aortic stenosis (AS) and heterotopic heart transplantation (HTX) in syngenic Lewis rats to induce mechanical overloading and unloading, respectively. Heart weight was 26±3% higher in AS (n = 7) and 33±2% lower in HTX (n = 7) as compared to sham-operated (n = 6) and healthy controls (n = 7). Small RNAs were enriched from the left ventricles and subjected to quantitative stem-loop specific RT-PCR targeting a panel of 351 miRNAs. In total, 153 miRNAs could be unambiguously detected. Out of 72 miRNAs previously implicated in the cardiovascular system, 40 miRNAs were regulated in AS and/or HTX. Overall, HTX displayed a slightly broader activation pattern for moderately regulated miRNAs. Surprisingly, however, the regulation of individual miRNA expression was strikingly similar in direction and amplitude in AS and HTX with no miRNA being regulated in opposite direction. In contrast, fetal hearts from Lewis rats at embryonic day 18 exhibited an entirely different miRNA expression pattern.<h4>Conclusions</h4>Taken together, our findings demonstrate that opposite changes in cardiac workload induce a common miRNA expression pattern which is markedly different from the fetal miRNA expression pattern. The direction of postnatal adaptive cardiac growth does, therefore, not appear to be determined at the level of single miRNAs or a specific set of miRNAs. Moreover, miRNAs themselves are not reprogrammed to a fetal program in response to changes in hemodynamic load.Ali El-ArmoucheAlexander Peter SchwoererChristiane NeuberJulius EmonsDaniel BiermannThomas ChristallaAdam GrundhoffThomas EschenhagenWolfram Hubertus ZimmermannHeimo EhmkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e14263 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ali El-Armouche Alexander Peter Schwoerer Christiane Neuber Julius Emons Daniel Biermann Thomas Christalla Adam Grundhoff Thomas Eschenhagen Wolfram Hubertus Zimmermann Heimo Ehmke Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
description	<h4>Background</h4>Mechanical overload leads to cardiac hypertrophy and mechanical unloading to cardiac atrophy. Both conditions produce similar transcriptional changes including a re-expression of fetal genes, despite obvious differences in phenotype. MicroRNAs (miRNAs) are discussed as superordinate regulators of global gene networks acting mainly at the translational level. Here, we hypothesized that defined sets of miRNAs may determine the direction of cardiomyocyte plasticity responses.<h4>Methodology/principal findings</h4>We employed ascending aortic stenosis (AS) and heterotopic heart transplantation (HTX) in syngenic Lewis rats to induce mechanical overloading and unloading, respectively. Heart weight was 26±3% higher in AS (n = 7) and 33±2% lower in HTX (n = 7) as compared to sham-operated (n = 6) and healthy controls (n = 7). Small RNAs were enriched from the left ventricles and subjected to quantitative stem-loop specific RT-PCR targeting a panel of 351 miRNAs. In total, 153 miRNAs could be unambiguously detected. Out of 72 miRNAs previously implicated in the cardiovascular system, 40 miRNAs were regulated in AS and/or HTX. Overall, HTX displayed a slightly broader activation pattern for moderately regulated miRNAs. Surprisingly, however, the regulation of individual miRNA expression was strikingly similar in direction and amplitude in AS and HTX with no miRNA being regulated in opposite direction. In contrast, fetal hearts from Lewis rats at embryonic day 18 exhibited an entirely different miRNA expression pattern.<h4>Conclusions</h4>Taken together, our findings demonstrate that opposite changes in cardiac workload induce a common miRNA expression pattern which is markedly different from the fetal miRNA expression pattern. The direction of postnatal adaptive cardiac growth does, therefore, not appear to be determined at the level of single miRNAs or a specific set of miRNAs. Moreover, miRNAs themselves are not reprogrammed to a fetal program in response to changes in hemodynamic load.
format	article
author	Ali El-Armouche Alexander Peter Schwoerer Christiane Neuber Julius Emons Daniel Biermann Thomas Christalla Adam Grundhoff Thomas Eschenhagen Wolfram Hubertus Zimmermann Heimo Ehmke
author_facet	Ali El-Armouche Alexander Peter Schwoerer Christiane Neuber Julius Emons Daniel Biermann Thomas Christalla Adam Grundhoff Thomas Eschenhagen Wolfram Hubertus Zimmermann Heimo Ehmke
author_sort	Ali El-Armouche
title	Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
title_short	Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
title_full	Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
title_fullStr	Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
title_full_unstemmed	Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
title_sort	common microrna signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f7b34f21c2c045f49d98f65bbcbf7772
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Common microRNA signatures in cardiac hypertrophic and atrophic remodeling induced by changes in hemodynamic load.

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