A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.

Detection of the Wolbachia endosymbiont in Aedes aegypti mosquitoes through real-time polymerase chain reaction assays is widely used during and after Wolbachia releases in dengue reduction trials involving the wMel and wAlbB strains. Although several different primer pairs have been applied in curr...

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Autores principales:	Meng-Jia Lau, Ary A Hoffmann, Nancy M Endersby-Harshman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7c12937f91a46bd8906ce5177ee5154
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spelling	oai:doaj.org-article:f7c12937f91a46bd8906ce5177ee51542021-12-02T20:14:14ZA diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.1932-620310.1371/journal.pone.0257781https://doaj.org/article/f7c12937f91a46bd8906ce5177ee51542021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257781https://doaj.org/toc/1932-6203Detection of the Wolbachia endosymbiont in Aedes aegypti mosquitoes through real-time polymerase chain reaction assays is widely used during and after Wolbachia releases in dengue reduction trials involving the wMel and wAlbB strains. Although several different primer pairs have been applied in current successful Wolbachia releases, they cannot be used in a single assay to distinguish between these strains. Here, we developed a new diagnostic primer pair, wMwA, which can detect the wMel or wAlbB infection in the same assay. We also tested current Wolbachia primers and show that there is variation in their performance when they are used to assess the relative density of Wolbachia. The new wMwA primers provide an accurate and efficient estimate of the presence and density of both Wolbachia infections, with practical implications for Wolbachia estimates in field collected Ae. aegypti where Wolbachia releases have taken place.Meng-Jia LauAry A HoffmannNancy M Endersby-HarshmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257781 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meng-Jia Lau Ary A Hoffmann Nancy M Endersby-Harshman A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.
description	Detection of the Wolbachia endosymbiont in Aedes aegypti mosquitoes through real-time polymerase chain reaction assays is widely used during and after Wolbachia releases in dengue reduction trials involving the wMel and wAlbB strains. Although several different primer pairs have been applied in current successful Wolbachia releases, they cannot be used in a single assay to distinguish between these strains. Here, we developed a new diagnostic primer pair, wMwA, which can detect the wMel or wAlbB infection in the same assay. We also tested current Wolbachia primers and show that there is variation in their performance when they are used to assess the relative density of Wolbachia. The new wMwA primers provide an accurate and efficient estimate of the presence and density of both Wolbachia infections, with practical implications for Wolbachia estimates in field collected Ae. aegypti where Wolbachia releases have taken place.
format	article
author	Meng-Jia Lau Ary A Hoffmann Nancy M Endersby-Harshman
author_facet	Meng-Jia Lau Ary A Hoffmann Nancy M Endersby-Harshman
author_sort	Meng-Jia Lau
title	A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.
title_short	A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.
title_full	A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.
title_fullStr	A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.
title_full_unstemmed	A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.
title_sort	diagnostic primer pair to distinguish between wmel and walbb wolbachia infections.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f7c12937f91a46bd8906ce5177ee5154
work_keys_str_mv	AT mengjialau adiagnosticprimerpairtodistinguishbetweenwmelandwalbbwolbachiainfections AT aryahoffmann adiagnosticprimerpairtodistinguishbetweenwmelandwalbbwolbachiainfections AT nancymendersbyharshman adiagnosticprimerpairtodistinguishbetweenwmelandwalbbwolbachiainfections AT mengjialau diagnosticprimerpairtodistinguishbetweenwmelandwalbbwolbachiainfections AT aryahoffmann diagnosticprimerpairtodistinguishbetweenwmelandwalbbwolbachiainfections AT nancymendersbyharshman diagnosticprimerpairtodistinguishbetweenwmelandwalbbwolbachiainfections
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A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infections.

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