Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China

Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse t...

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Autores principales:	Xiuling Yang, Min Du, Shupeng Li, Xueping Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mixed infection cotton leaf roll virus watermelon mosaic virus polerovirus potyvirus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f7c1a8cd2ffa4aa998e947e27ada422a
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spelling	oai:doaj.org-article:f7c1a8cd2ffa4aa998e947e27ada422a2021-11-25T19:13:22ZCoinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China10.3390/v131122101999-4915https://doaj.org/article/f7c1a8cd2ffa4aa998e947e27ada422a2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2210https://doaj.org/toc/1999-4915Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family <i>Solemoviridae</i>. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”.Xiuling YangMin DuShupeng LiXueping ZhouMDPI AGarticlemixed infectioncotton leaf roll viruswatermelon mosaic viruspoleroviruspotyvirusMicrobiologyQR1-502ENViruses, Vol 13, Iss 2210, p 2210 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mixed infection cotton leaf roll virus watermelon mosaic virus polerovirus potyvirus Microbiology QR1-502
spellingShingle	mixed infection cotton leaf roll virus watermelon mosaic virus polerovirus potyvirus Microbiology QR1-502 Xiuling Yang Min Du Shupeng Li Xueping Zhou Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
description	Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family <i>Solemoviridae</i>. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”.
format	article
author	Xiuling Yang Min Du Shupeng Li Xueping Zhou
author_facet	Xiuling Yang Min Du Shupeng Li Xueping Zhou
author_sort	Xiuling Yang
title	Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_short	Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_full	Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_fullStr	Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_full_unstemmed	Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_sort	coinfection of cotton plants with watermelon mosaic virus and a novel polerovirus in china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f7c1a8cd2ffa4aa998e947e27ada422a
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_version_	1718410192393076736

Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China

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