PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds

Di Mei,1,&ast; Xiaolong Guo,2,&ast; Yirong Wang,1 Xiaofei Huang,1 Li Guo,1 Pengfei Zou,3 Delong Ge,1 Xinxin Wang,1 Wenhui Lee,4 Tongyi Sun,1 Zhiqin Gao,1 Yuanyuan Gao3 1School of Life Science and Technology, Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandon...

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Autores principales:	Mei D, Guo X, Wang Y, Huang XF, Guo L, Zou P, Ge D, Wang X, Lee W, Sun T, Gao Z, Gao Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	oh-cath30 bacterial infection graphene oxide skin wound Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f7d0234ba57b4ebcb9969cf3f5f2eb90
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spelling	oai:doaj.org-article:f7d0234ba57b4ebcb9969cf3f5f2eb902021-12-02T16:11:31ZPEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds1178-2013https://doaj.org/article/f7d0234ba57b4ebcb9969cf3f5f2eb902021-07-01T00:00:00Zhttps://www.dovepress.com/pegylated-graphene-oxide-carried-oh-cath30-to-accelerate-the-healing-o-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Di Mei,1,&ast; Xiaolong Guo,2,&ast; Yirong Wang,1 Xiaofei Huang,1 Li Guo,1 Pengfei Zou,3 Delong Ge,1 Xinxin Wang,1 Wenhui Lee,4 Tongyi Sun,1 Zhiqin Gao,1 Yuanyuan Gao3 1School of Life Science and Technology, Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biopharmaceuticals, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of China; 2School of Basic Medicine, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of China; 3School of Pharmacy, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of China; 4Key Laboratory of Animal Models and Human Disease Mechanism, Institute of Zoology, Kunming, Yunnan, 650233, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Zhiqin GaoSchool of Life Science and Technology, Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biopharmaceuticals, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of ChinaTel +86 5368462066Email zhiqingao2013@163.comYuanyuan GaoSchool of Pharmacy, Weifang Medical University, Baotong Road, Weifang, Shandong Province, 261053, People’s Republic of ChinaTel +86 536 846 2067Email yyg20062006@126.comBackground: The treatment of Staphylococcus aureus (S. aureus)-infected wounds is difficult. It causes extreme pain to tens of thousands of patients and increases the cost of medical care. The antimicrobial peptide OH-CATH30 (OH30) has a good killing activity against S. aureus and can play a role in accelerating wound healing and immune regulation. Therefore, it shows great potential for wound healing.Purpose: The aim of this study was to overcome the short half-life and easy enzymolysis of OH30 by using graphene oxide conjugated with polyethylene glycol to load OH30 (denoted as PGO-OH30), as well as to evaluate its effect on wounds infected by S. aureus.Methods: PGO-OH30 nanoparticles were prepared by π–π conjugation and characterized. Their cell cytotoxicity, cell migration, infectious full-thickness dermotomy models, and histopathology were evaluated.Results: Characterization and cytotoxicity experiments revealed that the PGO-OH30 drug-delivery system had good biocompatibility and excellent drug-delivery ability. Cell-migration experiments showed that PGO-OH30 could promote the migration of human immortalized keratinocytes (HaCaT) cells compared with the control group (P< 0.05). In a mouse model of skin wound infection, PGO-OH30 accelerated skin-wound healing and reduced the amount of S. aureus in wounds compared with the control group (P< 0.05). In particular, on day 7, the number of S. aureus was 100 times lower in the PGO-OH30 group than in the control group.Conclusion: The PGO-OH30 drug-delivery system had good biocompatibility and excellent drug-delivery ability, indicating its good therapeutic effect on a skin wound-infection model.Keywords: OH-CATH30, bacterial infection, graphene oxide, skin woundMei DGuo XWang YHuang XFGuo LZou PGe DWang XLee WSun TGao ZGao YDove Medical Pressarticleoh-cath30bacterial infectiongraphene oxideskin woundMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 4769-4780 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oh-cath30 bacterial infection graphene oxide skin wound Medicine (General) R5-920
spellingShingle	oh-cath30 bacterial infection graphene oxide skin wound Medicine (General) R5-920 Mei D Guo X Wang Y Huang XF Guo L Zou P Ge D Wang X Lee W Sun T Gao Z Gao Y PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds
description	Di Mei,1,&ast; Xiaolong Guo,2,&ast; Yirong Wang,1 Xiaofei Huang,1 Li Guo,1 Pengfei Zou,3 Delong Ge,1 Xinxin Wang,1 Wenhui Lee,4 Tongyi Sun,1 Zhiqin Gao,1 Yuanyuan Gao3 1School of Life Science and Technology, Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biopharmaceuticals, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of China; 2School of Basic Medicine, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of China; 3School of Pharmacy, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of China; 4Key Laboratory of Animal Models and Human Disease Mechanism, Institute of Zoology, Kunming, Yunnan, 650233, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Zhiqin GaoSchool of Life Science and Technology, Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biopharmaceuticals, Weifang Medical University, Weifang, Shandong, 261053, People’s Republic of ChinaTel +86 5368462066Email zhiqingao2013@163.comYuanyuan GaoSchool of Pharmacy, Weifang Medical University, Baotong Road, Weifang, Shandong Province, 261053, People’s Republic of ChinaTel +86 536 846 2067Email yyg20062006@126.comBackground: The treatment of Staphylococcus aureus (S. aureus)-infected wounds is difficult. It causes extreme pain to tens of thousands of patients and increases the cost of medical care. The antimicrobial peptide OH-CATH30 (OH30) has a good killing activity against S. aureus and can play a role in accelerating wound healing and immune regulation. Therefore, it shows great potential for wound healing.Purpose: The aim of this study was to overcome the short half-life and easy enzymolysis of OH30 by using graphene oxide conjugated with polyethylene glycol to load OH30 (denoted as PGO-OH30), as well as to evaluate its effect on wounds infected by S. aureus.Methods: PGO-OH30 nanoparticles were prepared by π–π conjugation and characterized. Their cell cytotoxicity, cell migration, infectious full-thickness dermotomy models, and histopathology were evaluated.Results: Characterization and cytotoxicity experiments revealed that the PGO-OH30 drug-delivery system had good biocompatibility and excellent drug-delivery ability. Cell-migration experiments showed that PGO-OH30 could promote the migration of human immortalized keratinocytes (HaCaT) cells compared with the control group (P< 0.05). In a mouse model of skin wound infection, PGO-OH30 accelerated skin-wound healing and reduced the amount of S. aureus in wounds compared with the control group (P< 0.05). In particular, on day 7, the number of S. aureus was 100 times lower in the PGO-OH30 group than in the control group.Conclusion: The PGO-OH30 drug-delivery system had good biocompatibility and excellent drug-delivery ability, indicating its good therapeutic effect on a skin wound-infection model.Keywords: OH-CATH30, bacterial infection, graphene oxide, skin wound
format	article
author	Mei D Guo X Wang Y Huang XF Guo L Zou P Ge D Wang X Lee W Sun T Gao Z Gao Y
author_facet	Mei D Guo X Wang Y Huang XF Guo L Zou P Ge D Wang X Lee W Sun T Gao Z Gao Y
author_sort	Mei D
title	PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds
title_short	PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds
title_full	PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds
title_fullStr	PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds
title_full_unstemmed	PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds
title_sort	pegylated graphene oxide carried oh-cath30 to accelerate the healing of infected skin wounds
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/f7d0234ba57b4ebcb9969cf3f5f2eb90
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PEGylated Graphene Oxide Carried OH-CATH30 to Accelerate the Healing of Infected Skin Wounds

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