Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.

Polyploids account for approximately 70% of flowering plants, including many field, horticulture and forage crops. Cottons are a world-leading fiber and important oilseed crop, and a model species for study of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. This study has ad...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Meiping Zhang, Yang Zhang, James J Huang, Xiaojun Zhang, Mi-Kyung Lee, David M Stelly, Hong-Bin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7daf2e90b364ebab7adb0ad2f654082
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f7daf2e90b364ebab7adb0ad2f654082
record_format	dspace
spelling	oai:doaj.org-article:f7daf2e90b364ebab7adb0ad2f6540822021-11-18T07:24:56ZGenome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.1932-620310.1371/journal.pone.0033644https://doaj.org/article/f7daf2e90b364ebab7adb0ad2f6540822012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22438974/?tool=EBIhttps://doaj.org/toc/1932-6203Polyploids account for approximately 70% of flowering plants, including many field, horticulture and forage crops. Cottons are a world-leading fiber and important oilseed crop, and a model species for study of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. This study has addressed the concerns of physical mapping of polyploids with BACs and/or BIBACs by constructing a physical map of the tetraploid cotton, Gossypium hirsutum L. The physical map consists of 3,450 BIBAC contigs with an N50 contig size of 863 kb, collectively spanning 2,244 Mb. We sorted the map contigs according to their origin of subgenome, showing that we assembled physical maps for the A- and D-subgenomes of the tetraploid cotton, separately. We also identified the BIBACs in the map minimal tilling path, which consists of 15,277 clones. Moreover, we have marked the physical map with nearly 10,000 BIBAC ends (BESs), making one BES in approximately 250 kb. This physical map provides a line of evidence and a strategy for physical mapping of polyploids, and a platform for advanced research of the tetraploid cotton genome, particularly fine mapping and cloning the cotton agronomic genes and QTLs, and sequencing and assembling the cotton genome using the modern next-generation sequencing technology.Meiping ZhangYang ZhangJames J HuangXiaojun ZhangMi-Kyung LeeDavid M StellyHong-Bin ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33644 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meiping Zhang Yang Zhang James J Huang Xiaojun Zhang Mi-Kyung Lee David M Stelly Hong-Bin Zhang Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.
description	Polyploids account for approximately 70% of flowering plants, including many field, horticulture and forage crops. Cottons are a world-leading fiber and important oilseed crop, and a model species for study of plant polyploidization, cellulose biosynthesis and cell wall biogenesis. This study has addressed the concerns of physical mapping of polyploids with BACs and/or BIBACs by constructing a physical map of the tetraploid cotton, Gossypium hirsutum L. The physical map consists of 3,450 BIBAC contigs with an N50 contig size of 863 kb, collectively spanning 2,244 Mb. We sorted the map contigs according to their origin of subgenome, showing that we assembled physical maps for the A- and D-subgenomes of the tetraploid cotton, separately. We also identified the BIBACs in the map minimal tilling path, which consists of 15,277 clones. Moreover, we have marked the physical map with nearly 10,000 BIBAC ends (BESs), making one BES in approximately 250 kb. This physical map provides a line of evidence and a strategy for physical mapping of polyploids, and a platform for advanced research of the tetraploid cotton genome, particularly fine mapping and cloning the cotton agronomic genes and QTLs, and sequencing and assembling the cotton genome using the modern next-generation sequencing technology.
format	article
author	Meiping Zhang Yang Zhang James J Huang Xiaojun Zhang Mi-Kyung Lee David M Stelly Hong-Bin Zhang
author_facet	Meiping Zhang Yang Zhang James J Huang Xiaojun Zhang Mi-Kyung Lee David M Stelly Hong-Bin Zhang
author_sort	Meiping Zhang
title	Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.
title_short	Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.
title_full	Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.
title_fullStr	Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.
title_full_unstemmed	Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.
title_sort	genome physical mapping of polyploids: a bibac physical map of cultivated tetraploid cotton, gossypium hirsutum l.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f7daf2e90b364ebab7adb0ad2f654082
work_keys_str_mv	AT meipingzhang genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml AT yangzhang genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml AT jamesjhuang genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml AT xiaojunzhang genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml AT mikyunglee genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml AT davidmstelly genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml AT hongbinzhang genomephysicalmappingofpolyploidsabibacphysicalmapofcultivatedtetraploidcottongossypiumhirsutuml
_version_	1718423510591733760

Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.

Ejemplares similares