High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

Abstract For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional micros...

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Autores principales:	Chao Zuo, Jiasong Sun, Jiaji Li, Jialin Zhang, Anand Asundi, Qian Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7e4e9155f0746c0b751aec1e1f46fb7
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spelling	oai:doaj.org-article:f7e4e9155f0746c0b751aec1e1f46fb72021-12-02T15:05:38ZHigh-resolution transport-of-intensity quantitative phase microscopy with annular illumination10.1038/s41598-017-06837-12045-2322https://doaj.org/article/f7e4e9155f0746c0b751aec1e1f46fb72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06837-1https://doaj.org/toc/2045-2322Abstract For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with circular illumination aperture, partial coherence tends to diminish the phase contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE imaging, resulting in strong low-frequency artifacts and compromised imaging resolution. Here, we demonstrate how these issues can be effectively addressed by replacing the conventional circular illumination aperture with an annular one. The matched annular illumination not only strongly boosts the phase contrast for low spatial frequencies, but significantly improves the practical imaging resolution to near the incoherent diffraction limit. By incorporating high-numerical aperture (NA) illumination as well as high-NA objective, it is shown, for the first time, that TIE phase imaging can achieve a transverse resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse imaging of in vitro Hela cells revealing cellular morphology and subcellular dynamics during cells mitosis and apoptosis is exemplified. Given its capability for high-resolution QPI as well as the compatibility with widely available brightfield microscopy hardware, the proposed approach is expected to be adopted by the wider biology and medicine community.Chao ZuoJiasong SunJiaji LiJialin ZhangAnand AsundiQian ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-22 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Zuo Jiasong Sun Jiaji Li Jialin Zhang Anand Asundi Qian Chen High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
description	Abstract For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with circular illumination aperture, partial coherence tends to diminish the phase contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE imaging, resulting in strong low-frequency artifacts and compromised imaging resolution. Here, we demonstrate how these issues can be effectively addressed by replacing the conventional circular illumination aperture with an annular one. The matched annular illumination not only strongly boosts the phase contrast for low spatial frequencies, but significantly improves the practical imaging resolution to near the incoherent diffraction limit. By incorporating high-numerical aperture (NA) illumination as well as high-NA objective, it is shown, for the first time, that TIE phase imaging can achieve a transverse resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse imaging of in vitro Hela cells revealing cellular morphology and subcellular dynamics during cells mitosis and apoptosis is exemplified. Given its capability for high-resolution QPI as well as the compatibility with widely available brightfield microscopy hardware, the proposed approach is expected to be adopted by the wider biology and medicine community.
format	article
author	Chao Zuo Jiasong Sun Jiaji Li Jialin Zhang Anand Asundi Qian Chen
author_facet	Chao Zuo Jiasong Sun Jiaji Li Jialin Zhang Anand Asundi Qian Chen
author_sort	Chao Zuo
title	High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
title_short	High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
title_full	High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
title_fullStr	High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
title_full_unstemmed	High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
title_sort	high-resolution transport-of-intensity quantitative phase microscopy with annular illumination
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f7e4e9155f0746c0b751aec1e1f46fb7
work_keys_str_mv	AT chaozuo highresolutiontransportofintensityquantitativephasemicroscopywithannularillumination AT jiasongsun highresolutiontransportofintensityquantitativephasemicroscopywithannularillumination AT jiajili highresolutiontransportofintensityquantitativephasemicroscopywithannularillumination AT jialinzhang highresolutiontransportofintensityquantitativephasemicroscopywithannularillumination AT anandasundi highresolutiontransportofintensityquantitativephasemicroscopywithannularillumination AT qianchen highresolutiontransportofintensityquantitativephasemicroscopywithannularillumination
_version_	1718388806216843264

High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

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