Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression

Background. Sinomenine has been reported to effectively repress the progression of lung cancer and breast cancer. However, the effects of sinomenine in bladder cancer are not well understood. The purpose of this study was to evaluate the effects of sinomenine in bladder cancer. Methods. The mRNA exp...

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Autores principales:	Haili Xu, Jiayan Dong, Junhui Hou, Rui Gao
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/f7e6fbd08ff24a3c959c403b9207dc77
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spelling	oai:doaj.org-article:f7e6fbd08ff24a3c959c403b9207dc772021-11-15T01:20:11ZSinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression1741-428810.1155/2021/4699529https://doaj.org/article/f7e6fbd08ff24a3c959c403b9207dc772021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/4699529https://doaj.org/toc/1741-4288Background. Sinomenine has been reported to effectively repress the progression of lung cancer and breast cancer. However, the effects of sinomenine in bladder cancer are not well understood. The purpose of this study was to evaluate the effects of sinomenine in bladder cancer. Methods. The mRNA expression of HEIH in bladder cancer cells was measured by RT-qPCR. T24 and SW780 cells were treated with sinomenine for 24 hours. Cell viability was detected by the MTT assay. Cell migration and invasion were detected by the transwell assay. Western blotting assay was performed to assess the protein expression of Bcl-2, Bax, and caspase-3. Results. Sinomenine significantly suppressed cell viability in T24 and SW780 cells. Moreover, cell migration and invasion were significantly inhibited by sinomenine. Sinomenine accelerated the expression of Bax and caspase-3 but decreased the expression of Bcl-2. HEIH was upregulated in bladder cancer cells compared with normal bladder epithelial cells. Besides this, we noticed that HEIH knockdown blocked cell proliferation, migration, and invasion but facilitated cell apoptosis in bladder cancer cells. Additionally, HEIH reversed the suppression of the progression induced by sinomenine. Conclusion. Sinomenine was observed to suppress cell progression of bladder cancer cells by inhibiting HEIH expression. Our findings suggested that the use of sinomenine might be an effective treatment for bladder cancer.Haili XuJiayan DongJunhui HouRui GaoHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Haili Xu Jiayan Dong Junhui Hou Rui Gao Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
description	Background. Sinomenine has been reported to effectively repress the progression of lung cancer and breast cancer. However, the effects of sinomenine in bladder cancer are not well understood. The purpose of this study was to evaluate the effects of sinomenine in bladder cancer. Methods. The mRNA expression of HEIH in bladder cancer cells was measured by RT-qPCR. T24 and SW780 cells were treated with sinomenine for 24 hours. Cell viability was detected by the MTT assay. Cell migration and invasion were detected by the transwell assay. Western blotting assay was performed to assess the protein expression of Bcl-2, Bax, and caspase-3. Results. Sinomenine significantly suppressed cell viability in T24 and SW780 cells. Moreover, cell migration and invasion were significantly inhibited by sinomenine. Sinomenine accelerated the expression of Bax and caspase-3 but decreased the expression of Bcl-2. HEIH was upregulated in bladder cancer cells compared with normal bladder epithelial cells. Besides this, we noticed that HEIH knockdown blocked cell proliferation, migration, and invasion but facilitated cell apoptosis in bladder cancer cells. Additionally, HEIH reversed the suppression of the progression induced by sinomenine. Conclusion. Sinomenine was observed to suppress cell progression of bladder cancer cells by inhibiting HEIH expression. Our findings suggested that the use of sinomenine might be an effective treatment for bladder cancer.
format	article
author	Haili Xu Jiayan Dong Junhui Hou Rui Gao
author_facet	Haili Xu Jiayan Dong Junhui Hou Rui Gao
author_sort	Haili Xu
title	Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
title_short	Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
title_full	Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
title_fullStr	Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
title_full_unstemmed	Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression
title_sort	sinomenine inhibits the progression of bladder cancer cells by downregulating lncrna-heih expression
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/f7e6fbd08ff24a3c959c403b9207dc77
work_keys_str_mv	AT hailixu sinomenineinhibitstheprogressionofbladdercancercellsbydownregulatinglncrnaheihexpression AT jiayandong sinomenineinhibitstheprogressionofbladdercancercellsbydownregulatinglncrnaheihexpression AT junhuihou sinomenineinhibitstheprogressionofbladdercancercellsbydownregulatinglncrnaheihexpression AT ruigao sinomenineinhibitstheprogressionofbladdercancercellsbydownregulatinglncrnaheihexpression
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Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression

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