Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays

Abstract The aim of this study was to observe the effects of strophanthin induced inhibition of the Na-/K-ATPase in liver cells using a magnetic resonance (MR) compatible bioreactor. A microcavity array with a high density three-dimensional cell culture served as a functional magnetic resonance imag...

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Autores principales:	Andreas Neubauer, Cordula Nies, Victor D. Schepkin, Ruomin Hu, Matthias Malzacher, Jorge Chacón-Caldera, David Thiele, Eric Gottwald, Lothar R. Schad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7f0095fdced44388a66b77409341c9f
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spelling	oai:doaj.org-article:f7f0095fdced44388a66b77409341c9f2021-12-02T12:32:31ZTracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays10.1038/s41598-017-04226-22045-2322https://doaj.org/article/f7f0095fdced44388a66b77409341c9f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04226-2https://doaj.org/toc/2045-2322Abstract The aim of this study was to observe the effects of strophanthin induced inhibition of the Na-/K-ATPase in liver cells using a magnetic resonance (MR) compatible bioreactor. A microcavity array with a high density three-dimensional cell culture served as a functional magnetic resonance imaging (MRI) phantom for sodium multi quantum (MQ) spectroscopy. Direct contrast enhanced (DCE) MRI revealed the homogenous distribution of biochemical substances inside the bioreactor. NMR experiments using advanced bioreactors have advantages with respect to having full control over a variety of physiological parameters such as temperature, gas composition and fluid flow. Simultaneous detection of single quantum (SQ) and triple quantum (TQ) MR signals improves accuracy and was achieved by application of a pulse sequence with a time proportional phase increment (TQTPPI). The time course of the Na-/K-ATPase inhibition in the cell culture was demonstrated by the corresponding alterations of sodium TQ/SQ MR signals.Andreas NeubauerCordula NiesVictor D. SchepkinRuomin HuMatthias MalzacherJorge Chacón-CalderaDavid ThieleEric GottwaldLothar R. SchadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreas Neubauer Cordula Nies Victor D. Schepkin Ruomin Hu Matthias Malzacher Jorge Chacón-Caldera David Thiele Eric Gottwald Lothar R. Schad Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays
description	Abstract The aim of this study was to observe the effects of strophanthin induced inhibition of the Na-/K-ATPase in liver cells using a magnetic resonance (MR) compatible bioreactor. A microcavity array with a high density three-dimensional cell culture served as a functional magnetic resonance imaging (MRI) phantom for sodium multi quantum (MQ) spectroscopy. Direct contrast enhanced (DCE) MRI revealed the homogenous distribution of biochemical substances inside the bioreactor. NMR experiments using advanced bioreactors have advantages with respect to having full control over a variety of physiological parameters such as temperature, gas composition and fluid flow. Simultaneous detection of single quantum (SQ) and triple quantum (TQ) MR signals improves accuracy and was achieved by application of a pulse sequence with a time proportional phase increment (TQTPPI). The time course of the Na-/K-ATPase inhibition in the cell culture was demonstrated by the corresponding alterations of sodium TQ/SQ MR signals.
format	article
author	Andreas Neubauer Cordula Nies Victor D. Schepkin Ruomin Hu Matthias Malzacher Jorge Chacón-Caldera David Thiele Eric Gottwald Lothar R. Schad
author_facet	Andreas Neubauer Cordula Nies Victor D. Schepkin Ruomin Hu Matthias Malzacher Jorge Chacón-Caldera David Thiele Eric Gottwald Lothar R. Schad
author_sort	Andreas Neubauer
title	Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays
title_short	Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays
title_full	Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays
title_fullStr	Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays
title_full_unstemmed	Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays
title_sort	tracking protein function with sodium multi quantum spectroscopy in a 3d-tissue culture based on microcavity arrays
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f7f0095fdced44388a66b77409341c9f
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Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays

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