Seasonality modifies methylation profiles in healthy people.

DNA methylation is a well-characterized epigenetic modification that plays an important role in the regulation of gene expression. There is growing evidence on the involvement of epigenetic mechanisms in disease onset, including cancer. Environmental factors seem to induce changes in DNA methylation...

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Autores principales:	Fulvio Ricceri, Morena Trevisan, Valentina Fiano, Chiara Grasso, Francesca Fasanelli, Chiara Scoccianti, Laura De Marco, Anna Gillio Tos, Paolo Vineis, Carlotta Sacerdote
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7f429c42f4745648aa9532d5c74ab7f
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spelling	oai:doaj.org-article:f7f429c42f4745648aa9532d5c74ab7f2021-11-25T06:01:04ZSeasonality modifies methylation profiles in healthy people.1932-620310.1371/journal.pone.0106846https://doaj.org/article/f7f429c42f4745648aa9532d5c74ab7f2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106846https://doaj.org/toc/1932-6203DNA methylation is a well-characterized epigenetic modification that plays an important role in the regulation of gene expression. There is growing evidence on the involvement of epigenetic mechanisms in disease onset, including cancer. Environmental factors seem to induce changes in DNA methylation affecting human health. However, little is known about basal methylation levels in healthy people and about the correlation between environmental factors and different methylation profiles. We investigated the effect of seasonality on basal methylation by testing methylation levels in the long interspersed nucleotide element-1 (LINE-1) and in two cancer-related genes (RASSF1A and MGMT) of 88 healthy male heavy smokers involved in an Italian randomized study; at enrolment the subjects donated a blood sample collected in different months. Methylation analyses were performed by pyrosequencing. Mean methylation percentage was higher in spring and summer for the LINE1, RASSF1A and MGMT genes (68.26%, 2.35%, and 9.52% respectively) compared with autumn and winter (67.43%, 2.17%, and 8.60% respectively). In particular, LINE-1 was significantly hypomethylated (p = 0.04 or 0.05 depending on the CpG island involved) in autumn and winter compared with spring and summer. Seasonality seems to be a modifier of methylation levels and this observation should be taken into account in future analyses.Fulvio RicceriMorena TrevisanValentina FianoChiara GrassoFrancesca FasanelliChiara ScocciantiLaura De MarcoAnna Gillio TosPaolo VineisCarlotta SacerdotePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106846 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fulvio Ricceri Morena Trevisan Valentina Fiano Chiara Grasso Francesca Fasanelli Chiara Scoccianti Laura De Marco Anna Gillio Tos Paolo Vineis Carlotta Sacerdote Seasonality modifies methylation profiles in healthy people.
description	DNA methylation is a well-characterized epigenetic modification that plays an important role in the regulation of gene expression. There is growing evidence on the involvement of epigenetic mechanisms in disease onset, including cancer. Environmental factors seem to induce changes in DNA methylation affecting human health. However, little is known about basal methylation levels in healthy people and about the correlation between environmental factors and different methylation profiles. We investigated the effect of seasonality on basal methylation by testing methylation levels in the long interspersed nucleotide element-1 (LINE-1) and in two cancer-related genes (RASSF1A and MGMT) of 88 healthy male heavy smokers involved in an Italian randomized study; at enrolment the subjects donated a blood sample collected in different months. Methylation analyses were performed by pyrosequencing. Mean methylation percentage was higher in spring and summer for the LINE1, RASSF1A and MGMT genes (68.26%, 2.35%, and 9.52% respectively) compared with autumn and winter (67.43%, 2.17%, and 8.60% respectively). In particular, LINE-1 was significantly hypomethylated (p = 0.04 or 0.05 depending on the CpG island involved) in autumn and winter compared with spring and summer. Seasonality seems to be a modifier of methylation levels and this observation should be taken into account in future analyses.
format	article
author	Fulvio Ricceri Morena Trevisan Valentina Fiano Chiara Grasso Francesca Fasanelli Chiara Scoccianti Laura De Marco Anna Gillio Tos Paolo Vineis Carlotta Sacerdote
author_facet	Fulvio Ricceri Morena Trevisan Valentina Fiano Chiara Grasso Francesca Fasanelli Chiara Scoccianti Laura De Marco Anna Gillio Tos Paolo Vineis Carlotta Sacerdote
author_sort	Fulvio Ricceri
title	Seasonality modifies methylation profiles in healthy people.
title_short	Seasonality modifies methylation profiles in healthy people.
title_full	Seasonality modifies methylation profiles in healthy people.
title_fullStr	Seasonality modifies methylation profiles in healthy people.
title_full_unstemmed	Seasonality modifies methylation profiles in healthy people.
title_sort	seasonality modifies methylation profiles in healthy people.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f7f429c42f4745648aa9532d5c74ab7f
work_keys_str_mv	AT fulvioricceri seasonalitymodifiesmethylationprofilesinhealthypeople AT morenatrevisan seasonalitymodifiesmethylationprofilesinhealthypeople AT valentinafiano seasonalitymodifiesmethylationprofilesinhealthypeople AT chiaragrasso seasonalitymodifiesmethylationprofilesinhealthypeople AT francescafasanelli seasonalitymodifiesmethylationprofilesinhealthypeople AT chiarascoccianti seasonalitymodifiesmethylationprofilesinhealthypeople AT laurademarco seasonalitymodifiesmethylationprofilesinhealthypeople AT annagilliotos seasonalitymodifiesmethylationprofilesinhealthypeople AT paolovineis seasonalitymodifiesmethylationprofilesinhealthypeople AT carlottasacerdote seasonalitymodifiesmethylationprofilesinhealthypeople
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Seasonality modifies methylation profiles in healthy people.

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