Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.

The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical par...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tatiana I Manukhova, Ekaterina A Evtushenko, Alexander L Ksenofontov, Alexander M Arutyunyan, Angelina O Kovalenko, Nikolai A Nikitin, Olga V Karpova
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7faef4de381408ca91e767aec693447
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f7faef4de381408ca91e767aec693447
record_format	dspace
spelling	oai:doaj.org-article:f7faef4de381408ca91e767aec6934472021-12-02T20:09:02ZThermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.1932-620310.1371/journal.pone.0255378https://doaj.org/article/f7faef4de381408ca91e767aec6934472021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255378https://doaj.org/toc/1932-6203The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs' structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes.Tatiana I ManukhovaEkaterina A EvtushenkoAlexander L KsenofontovAlexander M ArutyunyanAngelina O KovalenkoNikolai A NikitinOlga V KarpovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255378 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatiana I Manukhova Ekaterina A Evtushenko Alexander L Ksenofontov Alexander M Arutyunyan Angelina O Kovalenko Nikolai A Nikitin Olga V Karpova Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
description	The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs' structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes.
format	article
author	Tatiana I Manukhova Ekaterina A Evtushenko Alexander L Ksenofontov Alexander M Arutyunyan Angelina O Kovalenko Nikolai A Nikitin Olga V Karpova
author_facet	Tatiana I Manukhova Ekaterina A Evtushenko Alexander L Ksenofontov Alexander M Arutyunyan Angelina O Kovalenko Nikolai A Nikitin Olga V Karpova
author_sort	Tatiana I Manukhova
title	Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
title_short	Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
title_full	Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
title_fullStr	Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
title_full_unstemmed	Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
title_sort	thermal remodelling of alternanthera mosaic virus virions and virus-like particles into protein spherical particles.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f7faef4de381408ca91e767aec693447
work_keys_str_mv	AT tatianaimanukhova thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles AT ekaterinaaevtushenko thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles AT alexanderlksenofontov thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles AT alexandermarutyunyan thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles AT angelinaokovalenko thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles AT nikolaianikitin thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles AT olgavkarpova thermalremodellingofalternantheramosaicvirusvirionsandviruslikeparticlesintoproteinsphericalparticles
_version_	1718375159760420864

Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles.

Ejemplares similares