Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms

Background. Thymol and piperine are two naturally occurring bioactive compounds with several pharmacological activities. In this study, their antibiofilm potential either alone or in combination with three aminoglycoside antibiotics was evaluated against a biofilm of Klebsiella pneumoniae. Methods....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Borel Bisso Ndezo, Christian Ramsès Tokam Kuaté, Jean Paul Dzoyem
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f805714c07514a98869a694cba64dfc2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f805714c07514a98869a694cba64dfc2
record_format	dspace
spelling	oai:doaj.org-article:f805714c07514a98869a694cba64dfc22021-11-22T01:09:54ZSynergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms1918-149310.1155/2021/7029944https://doaj.org/article/f805714c07514a98869a694cba64dfc22021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/7029944https://doaj.org/toc/1918-1493Background. Thymol and piperine are two naturally occurring bioactive compounds with several pharmacological activities. In this study, their antibiofilm potential either alone or in combination with three aminoglycoside antibiotics was evaluated against a biofilm of Klebsiella pneumoniae. Methods. Determination of antimicrobial susceptibility was performed using the broth microdilution method. Biofilm formation was evaluated by the microtiter plate method. Antibiofilm activity was determined using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay. The combination studies were performed by the checkerboard microdilution method. Results. The minimum biofilm inhibitory concentration (MBIC) of streptomycin was reduced by 16- to 64-fold when used in combination with thymol, while the MBIC of kanamycin was reduced by 4-fold when combined with piperine. The minimum biofilm eradication concentration (MBEC) values of streptomycin, amikacin, and kanamycin were, respectively, 16- to 128-fold, 4- to 128-fold, and 8- to 256-fold higher than the planktonic minimum inhibitory concentration (MIC). Thymol combined with streptomycin or kanamycin showed synergic effects against the preformed biofilm with 16- to 64-fold reduction in the minimum biofilm eradication concentration values of each antibiotic in combination. Piperine acted also synergically with kanamycin with an 8- to 16-fold reduction in the minimum biofilm eradication concentration values of kanamycin in combination. Conclusion. The association of thymol with antibiotics showed a strong synergistic effect both in the inhibition of biofilm formation and the destruction of the preformed biofilm of K. pneumoniae. This study suggests that a combination of thymol with streptomycin, amikacin, or kanamycin could be a promising alternative therapy to overcome the problem of K. pneumoniae biofilm-associated infections.Borel Bisso NdezoChristian Ramsès Tokam KuatéJean Paul DzoyemHindawi LimitedarticleInfectious and parasitic diseasesRC109-216MicrobiologyQR1-502ENCanadian Journal of Infectious Diseases and Medical Microbiology, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Infectious and parasitic diseases RC109-216 Microbiology QR1-502
spellingShingle	Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Borel Bisso Ndezo Christian Ramsès Tokam Kuaté Jean Paul Dzoyem Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms
description	Background. Thymol and piperine are two naturally occurring bioactive compounds with several pharmacological activities. In this study, their antibiofilm potential either alone or in combination with three aminoglycoside antibiotics was evaluated against a biofilm of Klebsiella pneumoniae. Methods. Determination of antimicrobial susceptibility was performed using the broth microdilution method. Biofilm formation was evaluated by the microtiter plate method. Antibiofilm activity was determined using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay. The combination studies were performed by the checkerboard microdilution method. Results. The minimum biofilm inhibitory concentration (MBIC) of streptomycin was reduced by 16- to 64-fold when used in combination with thymol, while the MBIC of kanamycin was reduced by 4-fold when combined with piperine. The minimum biofilm eradication concentration (MBEC) values of streptomycin, amikacin, and kanamycin were, respectively, 16- to 128-fold, 4- to 128-fold, and 8- to 256-fold higher than the planktonic minimum inhibitory concentration (MIC). Thymol combined with streptomycin or kanamycin showed synergic effects against the preformed biofilm with 16- to 64-fold reduction in the minimum biofilm eradication concentration values of each antibiotic in combination. Piperine acted also synergically with kanamycin with an 8- to 16-fold reduction in the minimum biofilm eradication concentration values of kanamycin in combination. Conclusion. The association of thymol with antibiotics showed a strong synergistic effect both in the inhibition of biofilm formation and the destruction of the preformed biofilm of K. pneumoniae. This study suggests that a combination of thymol with streptomycin, amikacin, or kanamycin could be a promising alternative therapy to overcome the problem of K. pneumoniae biofilm-associated infections.
format	article
author	Borel Bisso Ndezo Christian Ramsès Tokam Kuaté Jean Paul Dzoyem
author_facet	Borel Bisso Ndezo Christian Ramsès Tokam Kuaté Jean Paul Dzoyem
author_sort	Borel Bisso Ndezo
title	Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms
title_short	Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms
title_full	Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms
title_fullStr	Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms
title_full_unstemmed	Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms
title_sort	synergistic antibiofilm efficacy of thymol and piperine in combination with three aminoglycoside antibiotics against klebsiella pneumoniae biofilms
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/f805714c07514a98869a694cba64dfc2
work_keys_str_mv	AT borelbissondezo synergisticantibiofilmefficacyofthymolandpiperineincombinationwiththreeaminoglycosideantibioticsagainstklebsiellapneumoniaebiofilms AT christianramsestokamkuate synergisticantibiofilmefficacyofthymolandpiperineincombinationwiththreeaminoglycosideantibioticsagainstklebsiellapneumoniaebiofilms AT jeanpauldzoyem synergisticantibiofilmefficacyofthymolandpiperineincombinationwiththreeaminoglycosideantibioticsagainstklebsiellapneumoniaebiofilms
_version_	1718418437598871552

Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms

Ejemplares similares