Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction

Yefei Li*, Yuyu Yao*, Zulong Sheng, Yanxiaoxiao Yang, Genshan Ma,Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China*These two authors contributed equally to this work.Purpose: Results for implantation efficiency and effective improvement of ca...

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Autores principales:	Li YF, Yao YY, Sheng ZL, Yang YX, Ma GS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f81b3021b3e049bf973c2ff4cd3612e5
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spelling	oai:doaj.org-article:f81b3021b3e049bf973c2ff4cd3612e52021-12-02T01:23:33ZDual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction1176-91141178-2013https://doaj.org/article/f81b3021b3e049bf973c2ff4cd3612e52011-04-01T00:00:00Zhttp://www.dovepress.com/dual-modal-tracking-of-transplanted-mesenchymal-stem-cells-after-myoca-a7147https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yefei Li, Yuyu Yao, Zulong Sheng, Yanxiaoxiao Yang, Genshan Ma,Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China*These two authors contributed equally to this work.Purpose: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI).Methods: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1&prime;-dioctadecyl-3,3,3&prime;,3&prime;-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7.Results: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points.Conclusion: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function.Keywords: stem cell tracking, superparamagnetic iron oxide, DiD, cardiac function, myocardial infarction Li YFYao YYSheng ZLYang YXMa GSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 815-823 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li YF Yao YY Sheng ZL Yang YX Ma GS Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
description	Yefei Li, Yuyu Yao, Zulong Sheng, Yanxiaoxiao Yang, Genshan Ma,Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu, China*These two authors contributed equally to this work.Purpose: Results for implantation efficiency and effective improvement of cardiac function in the field of mesenchymal stem cells (MSCs) are controversial. To attempt to clarify this debate, we utilized magnetic resonance imaging (MRI) and near-infrared optical imaging (OI) to explore the effects of different delivery modes of mesenchymal stem cells on cell retention time and cardiac function after myocardial infarction (MI).Methods: Rat MSCs were labeled with superparamagnetic iron oxide nanoparticles and 1, 1&prime;-dioctadecyl-3,3,3&prime;,3&prime;-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) for noninvasive cell tracking in a rat MI model. Rats underwent coronary artery ligation and were randomized into three experimental groups: intravenous (IV), intramyocardial (IM), and a control group. The first two groups referred to the route of delivery of the transplanted dual-labeled MSCs; whereas the control group was given an IV injection of serum-free medium one day post-MI. Cellular engraftment was determined 1 day and 7 days post cell delivery by measuring the iron and optical signals in explanted organs. Prussian blue staining and fluorescent microscopy were performed on histological sections for iron and DiD, respectively. Cardiac function was measured by echocardiography on day 7.Results: The cardiac function of the IM group increased significantly compared to the IV and control groups at day 7. In the IM group, labeled cells were visualized in the infracted heart by serial MRI, and the intensity by OI was significantly higher on day 1. In the IV group, the heart signals were significantly attenuated by dual-modal tracking at two time points, but the lung signals in OI were significantly stronger than the IM group at both time points.Conclusion: IM injection of MSCs increased cell engraftment within infarcted hearts and improved cardiac function after MI. However, IV infusion has a low efficacy due to the cell trapping in the lung. Therefore, direct injection may provide an advantage over IV, with regard to retention of stem cells and protection of cardiac function.Keywords: stem cell tracking, superparamagnetic iron oxide, DiD, cardiac function, myocardial infarction
format	article
author	Li YF Yao YY Sheng ZL Yang YX Ma GS
author_facet	Li YF Yao YY Sheng ZL Yang YX Ma GS
author_sort	Li YF
title	Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_short	Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_full	Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_fullStr	Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_full_unstemmed	Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
title_sort	dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/f81b3021b3e049bf973c2ff4cd3612e5
work_keys_str_mv	AT liyf dualmodaltrackingoftransplantedmesenchymalstemcellsaftermyocardialinfarction AT yaoyy dualmodaltrackingoftransplantedmesenchymalstemcellsaftermyocardialinfarction AT shengzl dualmodaltrackingoftransplantedmesenchymalstemcellsaftermyocardialinfarction AT yangyx dualmodaltrackingoftransplantedmesenchymalstemcellsaftermyocardialinfarction AT mags dualmodaltrackingoftransplantedmesenchymalstemcellsaftermyocardialinfarction
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Dual-modal tracking of transplanted mesenchymal stem cells after myocardial infarction

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