A novel rhabdovirus associated with acute hemorrhagic fever in central Africa.
Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt dis...
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2012
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oai:doaj.org-article:f81b7b1065074d209cd1ab084ec166ac2021-11-18T06:03:56ZA novel rhabdovirus associated with acute hemorrhagic fever in central Africa.1553-73661553-737410.1371/journal.ppat.1002924https://doaj.org/article/f81b7b1065074d209cd1ab084ec166ac2012-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028323/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, mucosal hemorrhage, and, in two patients, death within 3 days. BASV was detected in an acute serum sample from the lone survivor at a concentration of 1.09 × 10(6) RNA copies/mL, and 98.2% of the genome was subsequently de novo assembled from ≈ 140 million sequence reads. Phylogenetic analysis revealed that BASV is highly divergent and shares less than 34% amino acid identity with any other rhabdovirus. High convalescent neutralizing antibody titers of >1:1000 were detected in the survivor and an asymptomatic nurse directly caring for him, both of whom were health care workers, suggesting the potential for human-to-human transmission of BASV. The natural animal reservoir host or arthropod vector and precise mode of transmission for the virus remain unclear. BASV is an emerging human pathogen associated with acute hemorrhagic fever in Africa.Gilda GrardJoseph N FairDeanna LeeElizabeth SlikasImke SteffenJean-Jacques MuyembeTaylor SittlerNarayanan VeeraraghavanJ Graham RubyChunlin WangMaria MakuwaPrime MulembakaniRobert B TeshJonna MazetAnne W RimoinTravis TaylorBradley S SchneiderGraham SimmonsEric DelwartNathan D WolfeCharles Y ChiuEric M LeroyPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 9, p e1002924 (2012) |
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DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Gilda Grard Joseph N Fair Deanna Lee Elizabeth Slikas Imke Steffen Jean-Jacques Muyembe Taylor Sittler Narayanan Veeraraghavan J Graham Ruby Chunlin Wang Maria Makuwa Prime Mulembakani Robert B Tesh Jonna Mazet Anne W Rimoin Travis Taylor Bradley S Schneider Graham Simmons Eric Delwart Nathan D Wolfe Charles Y Chiu Eric M Leroy A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. |
| description |
Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, mucosal hemorrhage, and, in two patients, death within 3 days. BASV was detected in an acute serum sample from the lone survivor at a concentration of 1.09 × 10(6) RNA copies/mL, and 98.2% of the genome was subsequently de novo assembled from ≈ 140 million sequence reads. Phylogenetic analysis revealed that BASV is highly divergent and shares less than 34% amino acid identity with any other rhabdovirus. High convalescent neutralizing antibody titers of >1:1000 were detected in the survivor and an asymptomatic nurse directly caring for him, both of whom were health care workers, suggesting the potential for human-to-human transmission of BASV. The natural animal reservoir host or arthropod vector and precise mode of transmission for the virus remain unclear. BASV is an emerging human pathogen associated with acute hemorrhagic fever in Africa. |
| format |
article |
| author |
Gilda Grard Joseph N Fair Deanna Lee Elizabeth Slikas Imke Steffen Jean-Jacques Muyembe Taylor Sittler Narayanan Veeraraghavan J Graham Ruby Chunlin Wang Maria Makuwa Prime Mulembakani Robert B Tesh Jonna Mazet Anne W Rimoin Travis Taylor Bradley S Schneider Graham Simmons Eric Delwart Nathan D Wolfe Charles Y Chiu Eric M Leroy |
| author_facet |
Gilda Grard Joseph N Fair Deanna Lee Elizabeth Slikas Imke Steffen Jean-Jacques Muyembe Taylor Sittler Narayanan Veeraraghavan J Graham Ruby Chunlin Wang Maria Makuwa Prime Mulembakani Robert B Tesh Jonna Mazet Anne W Rimoin Travis Taylor Bradley S Schneider Graham Simmons Eric Delwart Nathan D Wolfe Charles Y Chiu Eric M Leroy |
| author_sort |
Gilda Grard |
| title |
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. |
| title_short |
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. |
| title_full |
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. |
| title_fullStr |
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. |
| title_full_unstemmed |
A novel rhabdovirus associated with acute hemorrhagic fever in central Africa. |
| title_sort |
novel rhabdovirus associated with acute hemorrhagic fever in central africa. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/f81b7b1065074d209cd1ab084ec166ac |
| work_keys_str_mv |
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