MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children

Background: This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity. Methods: Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec...

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Autores principales:	Yang Kang, Minghui Bai, Liling Deng, Linbo Fan, Xing Wang
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	Childhood asthma MicroRNA Proliferation Transforming growth factor β1 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/f82358d9f6834435a449285c3b2152ef
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spelling	oai:doaj.org-article:f82358d9f6834435a449285c3b2152ef2021-12-02T19:21:11ZMiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children10.18502/ijph.v50i10.74972251-60852251-6093https://doaj.org/article/f82358d9f6834435a449285c3b2152ef2021-10-01T00:00:00Zhttps://ijph.tums.ac.ir/index.php/ijph/article/view/23996https://doaj.org/toc/2251-6085https://doaj.org/toc/2251-6093 Background: This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity. Methods: Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec 2019 were included. Forty nine healthy children underwent physical examination at this time period were also enrolled. The miR-21 expression in peripheral blood serum was analyzed by qRT-PCR. The relationship between the expression and severity of asthma in children was explored by Spearman correlation analysis and ROC curve. Bronchial epithelial cell lines were cultured in vitro and divided into blank control group, negative control group and miR-21 inhibition and activation group. The changes of cell proliferation after treatment were detected by CCK-8 test in different groups. The expression of TGF-β1/Smad signaling pathway protein in cells was assessed by Western blot (WB). Results: Compared with that of healthy children, the miR-21 expression in peripheral blood serum of asthmatic children was higher (P<0.001). MiR-21 expression was positively correlated with the severity of illness (r=0.853, P<0.001). The results of cell experiments in vitro signified that miR-21 can promote the proliferation of bronchial epithelial cells, and may be involved in regulating the expression of TGF-β1/ Smad3 signaling pathway, thus affecting cell proliferation. Conclusion: miRNA-21 regulates the proliferation of bronchial epithelial cells by activating TGFβ1/Smad signaling pathway. And it is positively correlated with the severity of asthma in children. Yang KangMinghui BaiLiling DengLinbo FanXing WangTehran University of Medical SciencesarticleChildhood asthmaMicroRNAProliferationTransforming growth factor β1Public aspects of medicineRA1-1270ENIranian Journal of Public Health, Vol 50, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Childhood asthma MicroRNA Proliferation Transforming growth factor β1 Public aspects of medicine RA1-1270
spellingShingle	Childhood asthma MicroRNA Proliferation Transforming growth factor β1 Public aspects of medicine RA1-1270 Yang Kang Minghui Bai Liling Deng Linbo Fan Xing Wang MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children
description	Background: This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity. Methods: Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec 2019 were included. Forty nine healthy children underwent physical examination at this time period were also enrolled. The miR-21 expression in peripheral blood serum was analyzed by qRT-PCR. The relationship between the expression and severity of asthma in children was explored by Spearman correlation analysis and ROC curve. Bronchial epithelial cell lines were cultured in vitro and divided into blank control group, negative control group and miR-21 inhibition and activation group. The changes of cell proliferation after treatment were detected by CCK-8 test in different groups. The expression of TGF-β1/Smad signaling pathway protein in cells was assessed by Western blot (WB). Results: Compared with that of healthy children, the miR-21 expression in peripheral blood serum of asthmatic children was higher (P<0.001). MiR-21 expression was positively correlated with the severity of illness (r=0.853, P<0.001). The results of cell experiments in vitro signified that miR-21 can promote the proliferation of bronchial epithelial cells, and may be involved in regulating the expression of TGF-β1/ Smad3 signaling pathway, thus affecting cell proliferation. Conclusion: miRNA-21 regulates the proliferation of bronchial epithelial cells by activating TGFβ1/Smad signaling pathway. And it is positively correlated with the severity of asthma in children.
format	article
author	Yang Kang Minghui Bai Liling Deng Linbo Fan Xing Wang
author_facet	Yang Kang Minghui Bai Liling Deng Linbo Fan Xing Wang
author_sort	Yang Kang
title	MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children
title_short	MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children
title_full	MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children
title_fullStr	MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children
title_full_unstemmed	MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children
title_sort	mirna-21 regulates bronchial epithelial cell proliferation by activating tgfβ1/smad signaling pathway and its correlation with asthma severity in children
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/f82358d9f6834435a449285c3b2152ef
work_keys_str_mv	AT yangkang mirna21regulatesbronchialepithelialcellproliferationbyactivatingtgfb1smadsignalingpathwayanditscorrelationwithasthmaseverityinchildren AT minghuibai mirna21regulatesbronchialepithelialcellproliferationbyactivatingtgfb1smadsignalingpathwayanditscorrelationwithasthmaseverityinchildren AT lilingdeng mirna21regulatesbronchialepithelialcellproliferationbyactivatingtgfb1smadsignalingpathwayanditscorrelationwithasthmaseverityinchildren AT linbofan mirna21regulatesbronchialepithelialcellproliferationbyactivatingtgfb1smadsignalingpathwayanditscorrelationwithasthmaseverityinchildren AT xingwang mirna21regulatesbronchialepithelialcellproliferationbyactivatingtgfb1smadsignalingpathwayanditscorrelationwithasthmaseverityinchildren
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MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children

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