IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.

IgE antibodies, administered to mice together with their specific antigen, enhance antibody and CD4(+) T cell responses to this antigen. The effect is dependent on the low affinity receptor for IgE, CD23, and the receptor must be expressed on B cells. In vitro, IgE-antigen complexes are endocytosed...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Frida Henningsson, Zhoujie Ding, Joakim S Dahlin, Marius Linkevicius, Fredrik Carlsson, Kjell-Olov Grönvik, Jenny Hallgren, Birgitta Heyman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f82549b012fb4d72b9c2534bf51110c5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f82549b012fb4d72b9c2534bf51110c5
record_format	dspace
spelling	oai:doaj.org-article:f82549b012fb4d72b9c2534bf51110c52021-11-18T06:50:39ZIgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.1932-620310.1371/journal.pone.0021760https://doaj.org/article/f82549b012fb4d72b9c2534bf51110c52011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21765910/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203IgE antibodies, administered to mice together with their specific antigen, enhance antibody and CD4(+) T cell responses to this antigen. The effect is dependent on the low affinity receptor for IgE, CD23, and the receptor must be expressed on B cells. In vitro, IgE-antigen complexes are endocytosed via CD23 on B cells, which subsequently present the antigen to CD4(+) T cells. This mechanism has been suggested to explain also IgE-mediated enhancement of immune responses in vivo. We recently found that CD23(+) B cells capture IgE-antigen complexes in peripheral blood and rapidly transport them to B cell follicles in the spleen. This provides an alternative explanation for the requirement for CD23(+) B cells. The aim of the present study was to determine whether B-cell mediated antigen presentation of IgE-antigen complexes explains the enhancing effect of IgE on immune responses in vivo. The ability of spleen cells, taken from mice 1-4 h after immunization with IgE-antigen, to present antigen to specific CD4(+) T cells was analyzed. Antigen presentation was intact when spleens were depleted of CD19(+) cells (i.e., primarily B cells) but was severely impaired after depletion of CD11c(+) cells (i.e., primarily dendritic cells). In agreement with this, the ability of IgE to enhance proliferation of CD4(+) T cells was abolished in CD11c-DTR mice conditionally depleted of CD11c(+) cells. Finally, the lack of IgE-mediated enhancemen of CD4(+) T cell responses in CD23(-/-) mice could be rescued by transfer of MHC-II-compatible as well as by MHC-II-incompatible CD23(+) B cells. These findings argue against the idea that IgE-mediated enhancement of specific CD4(+) T cell responses in vivo is caused by increased antigen presentation by B cells. A model where CD23(+) B cells act as antigen transporting cells, delivering antigen to CD11c(+) cells for presentation to T cells is consistent with available experimental data.Frida HenningssonZhoujie DingJoakim S DahlinMarius LinkeviciusFredrik CarlssonKjell-Olov GrönvikJenny HallgrenBirgitta HeymanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e21760 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frida Henningsson Zhoujie Ding Joakim S Dahlin Marius Linkevicius Fredrik Carlsson Kjell-Olov Grönvik Jenny Hallgren Birgitta Heyman IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.
description	IgE antibodies, administered to mice together with their specific antigen, enhance antibody and CD4(+) T cell responses to this antigen. The effect is dependent on the low affinity receptor for IgE, CD23, and the receptor must be expressed on B cells. In vitro, IgE-antigen complexes are endocytosed via CD23 on B cells, which subsequently present the antigen to CD4(+) T cells. This mechanism has been suggested to explain also IgE-mediated enhancement of immune responses in vivo. We recently found that CD23(+) B cells capture IgE-antigen complexes in peripheral blood and rapidly transport them to B cell follicles in the spleen. This provides an alternative explanation for the requirement for CD23(+) B cells. The aim of the present study was to determine whether B-cell mediated antigen presentation of IgE-antigen complexes explains the enhancing effect of IgE on immune responses in vivo. The ability of spleen cells, taken from mice 1-4 h after immunization with IgE-antigen, to present antigen to specific CD4(+) T cells was analyzed. Antigen presentation was intact when spleens were depleted of CD19(+) cells (i.e., primarily B cells) but was severely impaired after depletion of CD11c(+) cells (i.e., primarily dendritic cells). In agreement with this, the ability of IgE to enhance proliferation of CD4(+) T cells was abolished in CD11c-DTR mice conditionally depleted of CD11c(+) cells. Finally, the lack of IgE-mediated enhancemen of CD4(+) T cell responses in CD23(-/-) mice could be rescued by transfer of MHC-II-compatible as well as by MHC-II-incompatible CD23(+) B cells. These findings argue against the idea that IgE-mediated enhancement of specific CD4(+) T cell responses in vivo is caused by increased antigen presentation by B cells. A model where CD23(+) B cells act as antigen transporting cells, delivering antigen to CD11c(+) cells for presentation to T cells is consistent with available experimental data.
format	article
author	Frida Henningsson Zhoujie Ding Joakim S Dahlin Marius Linkevicius Fredrik Carlsson Kjell-Olov Grönvik Jenny Hallgren Birgitta Heyman
author_facet	Frida Henningsson Zhoujie Ding Joakim S Dahlin Marius Linkevicius Fredrik Carlsson Kjell-Olov Grönvik Jenny Hallgren Birgitta Heyman
author_sort	Frida Henningsson
title	IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.
title_short	IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.
title_full	IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.
title_fullStr	IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.
title_full_unstemmed	IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.
title_sort	ige-mediated enhancement of cd4+ t cell responses in mice requires antigen presentation by cd11c+ cells and not by b cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f82549b012fb4d72b9c2534bf51110c5
work_keys_str_mv	AT fridahenningsson igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT zhoujieding igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT joakimsdahlin igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT mariuslinkevicius igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT fredrikcarlsson igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT kjellolovgronvik igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT jennyhallgren igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells AT birgittaheyman igemediatedenhancementofcd4tcellresponsesinmicerequiresantigenpresentationbycd11ccellsandnotbybcells
_version_	1718424330534125568

IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells.

Ejemplares similares