Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis

Abstract Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock...

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Autores principales:	Nelson Martínez-Matías, Nataliya Chorna, Sahily González-Crespo, Lilliam Villanueva, Ingrid Montes-Rodríguez, Loyda M. Melendez-Aponte, Abiel Roche-Lima, Kelvin Carrasquillo-Carrión, Ednalise Santiago-Cartagena, Brian C. Rymond, Mohan Babu, Igor Stagljar, José R. Rodríguez-Medina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f825aad1e0eb4f1d95efe613c725f24e
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spelling	oai:doaj.org-article:f825aad1e0eb4f1d95efe613c725f24e2021-12-02T14:23:23ZToward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis10.1038/s41598-021-86671-82045-2322https://doaj.org/article/f825aad1e0eb4f1d95efe613c725f24e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86671-8https://doaj.org/toc/2045-2322Abstract Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock proteins (HSPs), proteins associated with the formation of stress granules, and the phosphatase subunit of trehalose 6-phosphate synthase which suggests that mtl1Δ strains undergo intrinsic activation of a non-lethal heat stress response. Furthermore, quantitative global proteomic analysis conducted on TMT-labeled proteins combined with metabolome analysis revealed that mtl1Δ strains exhibit decreased levels of metabolites of carboxylic acid metabolism, decreased expression of anabolic enzymes and increased expression of catabolic enzymes involved in the metabolism of amino acids, with enhanced expression of mitochondrial respirasome proteins. These observations support the idea that Mtl1 protein controls the suppression of a non-lethal heat stress response under normal conditions while it plays an important role in metabolic regulatory mechanisms linked to TORC1 signaling that are required to maintain cellular homeostasis and optimal mitochondrial function.Nelson Martínez-MatíasNataliya ChornaSahily González-CrespoLilliam VillanuevaIngrid Montes-RodríguezLoyda M. Melendez-AponteAbiel Roche-LimaKelvin Carrasquillo-CarriónEdnalise Santiago-CartagenaBrian C. RymondMohan BabuIgor StagljarJosé R. Rodríguez-MedinaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nelson Martínez-Matías Nataliya Chorna Sahily González-Crespo Lilliam Villanueva Ingrid Montes-Rodríguez Loyda M. Melendez-Aponte Abiel Roche-Lima Kelvin Carrasquillo-Carrión Ednalise Santiago-Cartagena Brian C. Rymond Mohan Babu Igor Stagljar José R. Rodríguez-Medina Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis
description	Abstract Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock proteins (HSPs), proteins associated with the formation of stress granules, and the phosphatase subunit of trehalose 6-phosphate synthase which suggests that mtl1Δ strains undergo intrinsic activation of a non-lethal heat stress response. Furthermore, quantitative global proteomic analysis conducted on TMT-labeled proteins combined with metabolome analysis revealed that mtl1Δ strains exhibit decreased levels of metabolites of carboxylic acid metabolism, decreased expression of anabolic enzymes and increased expression of catabolic enzymes involved in the metabolism of amino acids, with enhanced expression of mitochondrial respirasome proteins. These observations support the idea that Mtl1 protein controls the suppression of a non-lethal heat stress response under normal conditions while it plays an important role in metabolic regulatory mechanisms linked to TORC1 signaling that are required to maintain cellular homeostasis and optimal mitochondrial function.
format	article
author	Nelson Martínez-Matías Nataliya Chorna Sahily González-Crespo Lilliam Villanueva Ingrid Montes-Rodríguez Loyda M. Melendez-Aponte Abiel Roche-Lima Kelvin Carrasquillo-Carrión Ednalise Santiago-Cartagena Brian C. Rymond Mohan Babu Igor Stagljar José R. Rodríguez-Medina
author_facet	Nelson Martínez-Matías Nataliya Chorna Sahily González-Crespo Lilliam Villanueva Ingrid Montes-Rodríguez Loyda M. Melendez-Aponte Abiel Roche-Lima Kelvin Carrasquillo-Carrión Ednalise Santiago-Cartagena Brian C. Rymond Mohan Babu Igor Stagljar José R. Rodríguez-Medina
author_sort	Nelson Martínez-Matías
title	Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis
title_short	Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis
title_full	Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis
title_fullStr	Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis
title_full_unstemmed	Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis
title_sort	toward the discovery of biological functions associated with the mechanosensor mtl1p of saccharomyces cerevisiae via integrative multi-omics analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f825aad1e0eb4f1d95efe613c725f24e
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Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis

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