UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression
Chunmei Geng,*,1 Yujin Guo,*,1 Yi Qiao,2 Jun Zhang,3 Dan Chen,1 Wenxiu Han,1 Mengqi Yang,1 Pei Jiang11Institute of Clinical Pharmacy and Pharmacology, Jining First People’s Hospital, Jining Medical University, Jining, People’s Republic of China; 2Department of P...
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Dove Medical Press
2019
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oai:doaj.org-article:f832a59cac69443c8a18edfbf8b8cf472021-12-02T03:27:15ZUPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression1178-2021https://doaj.org/article/f832a59cac69443c8a18edfbf8b8cf472019-07-01T00:00:00Zhttps://www.dovepress.com/uplc-q-tof-ms-profiling-of-the-hippocampus-reveals-metabolite-biomarke-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Chunmei Geng,*,1 Yujin Guo,*,1 Yi Qiao,2 Jun Zhang,3 Dan Chen,1 Wenxiu Han,1 Mengqi Yang,1 Pei Jiang11Institute of Clinical Pharmacy and Pharmacology, Jining First People’s Hospital, Jining Medical University, Jining, People’s Republic of China; 2Department of Public Health, Jining Medical University, Jining, People’s Republic of China; 3Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China*These authors contributed equally to this workPurpose: An increasing body of evidence reveals that inflammation is involved in the pathological mechanisms of depression. Our previous basic research confirmed that Dl-3-n-butylphthalide (NBP) possess anti-inflammatory properties. However, studies investigating metabolite biomarkers for the involvement of NBP in hippocampus tissue in the lipopolysaccharide (LPS)-induced rat model of depression are currently limited. Thus, the aim of this study was to identify metabolite biomarkers in the hippocampus for the impact of NBP in this model of depression.Material and methods: Male Sprague–Dawley rats were randomly allocated to one of the following three groups (n=6): Control, LPS-induced rat model of depression (LPS), and NBP involvement in the LPS-induced rat model of depression (LPS+NBP). Ultra-high-performance liquid chromatography-mass spectroscopy was used to determine the hippocampal metabolites. Multivariate statistical analysis was performed to identify differentially expressed hippocampal metabolites in the three groups.Results: Most of the identified differentially expressed metabolites were related to amino acid, lipid, energy, and oxidative stress metabolism. Additionally, metabolites were eventually connected to different pathways and metabolic networks, which may partly account for the pathophysiological process of depression.Conclusion: The present findings provide insight into the anti-inflammatory effects of NBP, and further elucidate the pathophysiological mechanisms underlying inflammation-induced depression.Keywords: inflammation, Dl-3-n-butylphthalide, lipopolysaccharide, metabolite biomarkers, ultra-high-performance liquid chromatography-mass spectroscopyGeng CGuo YQiao YZhang JChen DHan WYang MJiang PDove Medical PressarticleinflammationDl-3-n-butylphthalidelipopolysaccharidemetabolite biomarkersultra-high-performance liquid chromatography-mass spectroscopyNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 1939-1950 (2019) |
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DOAJ |
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| topic |
inflammation Dl-3-n-butylphthalide lipopolysaccharide metabolite biomarkers ultra-high-performance liquid chromatography-mass spectroscopy Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 |
| spellingShingle |
inflammation Dl-3-n-butylphthalide lipopolysaccharide metabolite biomarkers ultra-high-performance liquid chromatography-mass spectroscopy Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Geng C Guo Y Qiao Y Zhang J Chen D Han W Yang M Jiang P UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| description |
Chunmei Geng,*,1 Yujin Guo,*,1 Yi Qiao,2 Jun Zhang,3 Dan Chen,1 Wenxiu Han,1 Mengqi Yang,1 Pei Jiang11Institute of Clinical Pharmacy and Pharmacology, Jining First People’s Hospital, Jining Medical University, Jining, People’s Republic of China; 2Department of Public Health, Jining Medical University, Jining, People’s Republic of China; 3Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China*These authors contributed equally to this workPurpose: An increasing body of evidence reveals that inflammation is involved in the pathological mechanisms of depression. Our previous basic research confirmed that Dl-3-n-butylphthalide (NBP) possess anti-inflammatory properties. However, studies investigating metabolite biomarkers for the involvement of NBP in hippocampus tissue in the lipopolysaccharide (LPS)-induced rat model of depression are currently limited. Thus, the aim of this study was to identify metabolite biomarkers in the hippocampus for the impact of NBP in this model of depression.Material and methods: Male Sprague–Dawley rats were randomly allocated to one of the following three groups (n=6): Control, LPS-induced rat model of depression (LPS), and NBP involvement in the LPS-induced rat model of depression (LPS+NBP). Ultra-high-performance liquid chromatography-mass spectroscopy was used to determine the hippocampal metabolites. Multivariate statistical analysis was performed to identify differentially expressed hippocampal metabolites in the three groups.Results: Most of the identified differentially expressed metabolites were related to amino acid, lipid, energy, and oxidative stress metabolism. Additionally, metabolites were eventually connected to different pathways and metabolic networks, which may partly account for the pathophysiological process of depression.Conclusion: The present findings provide insight into the anti-inflammatory effects of NBP, and further elucidate the pathophysiological mechanisms underlying inflammation-induced depression.Keywords: inflammation, Dl-3-n-butylphthalide, lipopolysaccharide, metabolite biomarkers, ultra-high-performance liquid chromatography-mass spectroscopy |
| format |
article |
| author |
Geng C Guo Y Qiao Y Zhang J Chen D Han W Yang M Jiang P |
| author_facet |
Geng C Guo Y Qiao Y Zhang J Chen D Han W Yang M Jiang P |
| author_sort |
Geng C |
| title |
UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| title_short |
UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| title_full |
UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| title_fullStr |
UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| title_full_unstemmed |
UPLC-Q-TOF-MS profiling of the hippocampus reveals metabolite biomarkers for the impact of Dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| title_sort |
uplc-q-tof-ms profiling of the hippocampus reveals metabolite biomarkers for the impact of dl-3-n-butylphthalide on the lipopolysaccharide-induced rat model of depression |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/f832a59cac69443c8a18edfbf8b8cf47 |
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