FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT

Abstract. To study influence of microenvironment upon functional activity of B cells, we used experimental models of adoptive cell transfer from CBA to congenic CBA/N mice lacking CD5+ B-1 cells, and cocultivation of CBA/N splenocytes with spleen, or peritoneal CBA cells. B cell activity was determi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	I. N. Dyakov, I. V. Grigoriev, E. V. Sidorova, I. N. Chernyshova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	в-lymphocytes local microenvironment immunoglobulins Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f832b58a852c4da5ab66211623b4a4f7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f832b58a852c4da5ab66211623b4a4f7
record_format	dspace
spelling	oai:doaj.org-article:f832b58a852c4da5ab66211623b4a4f72021-11-18T08:03:37ZFUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT1563-06252313-741X10.15789/1563-0625-2008-1-51-58https://doaj.org/article/f832b58a852c4da5ab66211623b4a4f72014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/80https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. To study influence of microenvironment upon functional activity of B cells, we used experimental models of adoptive cell transfer from CBA to congenic CBA/N mice lacking CD5+ B-1 cells, and cocultivation of CBA/N splenocytes with spleen, or peritoneal CBA cells. B cell activity was determined as numbers of IgM-producing cells, and as amounts of cells producing antibodies to a T-independent antigen type 2 (polyvinylpirrolidone). In vivo distribution of transferred cells was determined as the numbers of cells stained with a vital dye (CDFA-SE) in spleen and peritoneum of recipients. Intravenous injection of CBA splenocytes resulted into a significant (3- to 4-fold) increase in numbers of IgM-producing cells in the spleens of xid–recipients, where their levels reached those of CBA mice. Intravenous injection of CBA/N splenocytes into xid-mice did not induce any increase of IgM-producers in their spleen. That means that increased number of IgM-producers in recipient spleen is due to donor cells, presumably, CD5+ B-1 lymphocytes. Meanwhile, restoration of immune response to polyvinylpirrolidone in xid-mice following transfer of CBA splenocytes was not successful. IgM-producing cells were undetectable in peritoneum of intact mice (both CBA and CBA/N). Intraperitoneal transfer of CBA splenocytes also did not induce their accumulation. It could mean that peritoneal microenvironment inhibits B cell activity. Meanwhile, intravenous injection of «silent» peritoneal cells into CBA/N mice brought about great increase of IgM-producers in recipient spleen, i.e., the «job» of B cells was permitted in other microenvironment. The results yielded in vivo are in agreement with data of in vitro experiments. Addition of CBA splenocytes or peritoneal cells (10-50%) to CBA/N splenocytes induced sharp increase of IgM-producing cells in the cultures. The data obtained provide evidence for a decisive role of microenvironment in functional activity of murine B lymphocytes.I. N. DyakovI. V. GrigorievE. V. SidorovaI. N. ChernyshovaSPb RAACIarticleв-lymphocyteslocal microenvironmentimmunoglobulinsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 1, Pp 51-58 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	в-lymphocytes local microenvironment immunoglobulins Immunologic diseases. Allergy RC581-607
spellingShingle	в-lymphocytes local microenvironment immunoglobulins Immunologic diseases. Allergy RC581-607 I. N. Dyakov I. V. Grigoriev E. V. Sidorova I. N. Chernyshova FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
description	Abstract. To study influence of microenvironment upon functional activity of B cells, we used experimental models of adoptive cell transfer from CBA to congenic CBA/N mice lacking CD5+ B-1 cells, and cocultivation of CBA/N splenocytes with spleen, or peritoneal CBA cells. B cell activity was determined as numbers of IgM-producing cells, and as amounts of cells producing antibodies to a T-independent antigen type 2 (polyvinylpirrolidone). In vivo distribution of transferred cells was determined as the numbers of cells stained with a vital dye (CDFA-SE) in spleen and peritoneum of recipients. Intravenous injection of CBA splenocytes resulted into a significant (3- to 4-fold) increase in numbers of IgM-producing cells in the spleens of xid–recipients, where their levels reached those of CBA mice. Intravenous injection of CBA/N splenocytes into xid-mice did not induce any increase of IgM-producers in their spleen. That means that increased number of IgM-producers in recipient spleen is due to donor cells, presumably, CD5+ B-1 lymphocytes. Meanwhile, restoration of immune response to polyvinylpirrolidone in xid-mice following transfer of CBA splenocytes was not successful. IgM-producing cells were undetectable in peritoneum of intact mice (both CBA and CBA/N). Intraperitoneal transfer of CBA splenocytes also did not induce their accumulation. It could mean that peritoneal microenvironment inhibits B cell activity. Meanwhile, intravenous injection of «silent» peritoneal cells into CBA/N mice brought about great increase of IgM-producers in recipient spleen, i.e., the «job» of B cells was permitted in other microenvironment. The results yielded in vivo are in agreement with data of in vitro experiments. Addition of CBA splenocytes or peritoneal cells (10-50%) to CBA/N splenocytes induced sharp increase of IgM-producing cells in the cultures. The data obtained provide evidence for a decisive role of microenvironment in functional activity of murine B lymphocytes.
format	article
author	I. N. Dyakov I. V. Grigoriev E. V. Sidorova I. N. Chernyshova
author_facet	I. N. Dyakov I. V. Grigoriev E. V. Sidorova I. N. Chernyshova
author_sort	I. N. Dyakov
title	FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
title_short	FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
title_full	FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
title_fullStr	FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
title_full_unstemmed	FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
title_sort	functional activity of murine b cell: a role of microenvironment
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/f832b58a852c4da5ab66211623b4a4f7
work_keys_str_mv	AT indyakov functionalactivityofmurinebcellaroleofmicroenvironment AT ivgrigoriev functionalactivityofmurinebcellaroleofmicroenvironment AT evsidorova functionalactivityofmurinebcellaroleofmicroenvironment AT inchernyshova functionalactivityofmurinebcellaroleofmicroenvironment
_version_	1718422581541863424

FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT

Ejemplares similares