Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats

stromal/stem cells (hEnSCs) are mesenchymal stem cell (MSC)-like cells, which are suitable candidates for regenerative medicine purposes, yet the effect of hEnSCs on cisplatin-induced AKI has not been studied; therefore, the present study was conducted to investigate this gap in the literature. Mat...

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Autores principales:	Hadis Zeinali, Mahnaz Azarnia, Peyman Keyhanvar, Reza Moghadasali, Somayeh Ebrahimi-Barough
Formato:	article
Lenguaje:	EN
Publicado:	Royan Institute (ACECR), Tehran 2021
Materias:	acute kidney injury apoptosis cisplatin human endometrial stromal/stem cell Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8367e09e7604f5e9bc62d9a69fc5671
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spelling	oai:doaj.org-article:f8367e09e7604f5e9bc62d9a69fc56712021-11-07T08:00:27ZHuman Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats10.22074/cellj.2021.73222228-58062228-5814https://doaj.org/article/f8367e09e7604f5e9bc62d9a69fc56712021-10-01T00:00:00Zhttps://celljournal.org/journal/article/fulltext/human-endometrial-stromalstem-cells-protect-against-cisplatin-induced-acute-kidney-injury-by-inhibition-of-apoptosis-in-male-wistar-rats.pdfhttps://doaj.org/toc/2228-5806https://doaj.org/toc/2228-5814stromal/stem cells (hEnSCs) are mesenchymal stem cell (MSC)-like cells, which are suitable candidates for regenerative medicine purposes, yet the effect of hEnSCs on cisplatin-induced AKI has not been studied; therefore, the present study was conducted to investigate this gap in the literature. Materials and Methods: In this experimental study, hEnSCs were obtained from endometrial biopsy using collagenase I and were then cultured in DMEM/F12 medium. A total of 48 male Wistar rats (150-200 g) were classified into four groups: intact -receiving no treatment, model -receiving 5 mg/kg of body weight cisplatin, as well as phosphate-buffered saline (PBS) and cell -receiving either PBS or hEnSCs for three hours after cisplatin injection, respectively. Biochemical parameters, pathologic scores, apoptosis assay, Bcl-2 and Tnf-α expression were evaluated on day 5. Results: On day 5 post-transplantation we observed that HEnSCs injection has led to a decrease in both blood urea nitrogen (BUN) and serum creatinine (SCr), compared to the model and PBS groups (0.82 ± 0.03 vs. 1.42 ± 0.06, 1.09 ± 0.05 mg/dl and 61.53 ± 3.07 vs. 116.60 ± 2.12, 112.00 ± 1.35 mg/dl, respectively). The highest levels of pathologic scores were observed in model and PBS groups, while hEnSCs transplantation resulted in a decrease in pathologic scores (149.10 ± 7.03, 141.50 ± 4.68 vs. 118 ± 2.16). HEnSCs significantly decreased the percentage of TUNELpositive cells in the cell group compared with model and PBS groups (20.37 ±. 3.37 vs. 33.67 ± 1.79, 31.53 ± 1.05 in glomeruli and 15.10 ± 1.47 vs. 42.33 ± 1.72, 39.23 ± 1.61 in tubules). In addition, HEnSCs resulted in upregulation of Bcl-2 and downregulation of Tnf-α in the cisplatin-induced AKI. Conclusion: Our results showed that injection of hEnSCs may improve AKI through lowering the amount of apoptosis in renal cells.Hadis ZeinaliMahnaz AzarniaPeyman KeyhanvarReza MoghadasaliSomayeh Ebrahimi-BaroughRoyan Institute (ACECR), Tehranarticleacute kidney injuryapoptosiscisplatinhuman endometrial stromal/stem cellMedicineRScienceQENCell Journal, Vol 23, Iss 5, Pp 568-575 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acute kidney injury apoptosis cisplatin human endometrial stromal/stem cell Medicine R Science Q
spellingShingle	acute kidney injury apoptosis cisplatin human endometrial stromal/stem cell Medicine R Science Q Hadis Zeinali Mahnaz Azarnia Peyman Keyhanvar Reza Moghadasali Somayeh Ebrahimi-Barough Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats
description	stromal/stem cells (hEnSCs) are mesenchymal stem cell (MSC)-like cells, which are suitable candidates for regenerative medicine purposes, yet the effect of hEnSCs on cisplatin-induced AKI has not been studied; therefore, the present study was conducted to investigate this gap in the literature. Materials and Methods: In this experimental study, hEnSCs were obtained from endometrial biopsy using collagenase I and were then cultured in DMEM/F12 medium. A total of 48 male Wistar rats (150-200 g) were classified into four groups: intact -receiving no treatment, model -receiving 5 mg/kg of body weight cisplatin, as well as phosphate-buffered saline (PBS) and cell -receiving either PBS or hEnSCs for three hours after cisplatin injection, respectively. Biochemical parameters, pathologic scores, apoptosis assay, Bcl-2 and Tnf-α expression were evaluated on day 5. Results: On day 5 post-transplantation we observed that HEnSCs injection has led to a decrease in both blood urea nitrogen (BUN) and serum creatinine (SCr), compared to the model and PBS groups (0.82 ± 0.03 vs. 1.42 ± 0.06, 1.09 ± 0.05 mg/dl and 61.53 ± 3.07 vs. 116.60 ± 2.12, 112.00 ± 1.35 mg/dl, respectively). The highest levels of pathologic scores were observed in model and PBS groups, while hEnSCs transplantation resulted in a decrease in pathologic scores (149.10 ± 7.03, 141.50 ± 4.68 vs. 118 ± 2.16). HEnSCs significantly decreased the percentage of TUNELpositive cells in the cell group compared with model and PBS groups (20.37 ±. 3.37 vs. 33.67 ± 1.79, 31.53 ± 1.05 in glomeruli and 15.10 ± 1.47 vs. 42.33 ± 1.72, 39.23 ± 1.61 in tubules). In addition, HEnSCs resulted in upregulation of Bcl-2 and downregulation of Tnf-α in the cisplatin-induced AKI. Conclusion: Our results showed that injection of hEnSCs may improve AKI through lowering the amount of apoptosis in renal cells.
format	article
author	Hadis Zeinali Mahnaz Azarnia Peyman Keyhanvar Reza Moghadasali Somayeh Ebrahimi-Barough
author_facet	Hadis Zeinali Mahnaz Azarnia Peyman Keyhanvar Reza Moghadasali Somayeh Ebrahimi-Barough
author_sort	Hadis Zeinali
title	Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats
title_short	Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats
title_full	Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats
title_fullStr	Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats
title_full_unstemmed	Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats
title_sort	human endometrial stromal/stem cells inhibit apoptosis in cisplatin-induced acute kidney injury in male wistar rats
publisher	Royan Institute (ACECR), Tehran
publishDate	2021
url	https://doaj.org/article/f8367e09e7604f5e9bc62d9a69fc5671
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Human Endometrial Stromal/Stem Cells Inhibit Apoptosis in Cisplatin-Induced Acute Kidney Injury in Male Wistar Rats

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