Reproducibility challenges in the search for antibacterial compounds from nature.

<h4>Background</h4>Reproducibility of reported antibacterial activities of plant extracts has long remained questionable. Although plant-related factors should be well considered in serious pharmacognostic research, they are often not addressed in many research papers. Here we highlight...

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Autores principales:	Nelson E Masota, Gerd Vogg, Knut Ohlsen, Ulrike Holzgrabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8372c036c474dfc88c32b3c8ddcec36
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spelling	oai:doaj.org-article:f8372c036c474dfc88c32b3c8ddcec362021-12-02T20:08:53ZReproducibility challenges in the search for antibacterial compounds from nature.1932-620310.1371/journal.pone.0255437https://doaj.org/article/f8372c036c474dfc88c32b3c8ddcec362021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255437https://doaj.org/toc/1932-6203<h4>Background</h4>Reproducibility of reported antibacterial activities of plant extracts has long remained questionable. Although plant-related factors should be well considered in serious pharmacognostic research, they are often not addressed in many research papers. Here we highlight the challenges in reproducing antibacterial activities of plant extracts.<h4>Methods</h4>Plants with reported antibacterial activities of interest were obtained from a literature review. Antibacterial activities against Escherichia coli and Klebsiella pneumoniae were tested using extracts' solutions in 10% DMSO and acetone. Compositions of working solutions from both solvents were established using LC-MS analysis. Moreover, the availability of details likely to affect reproducibility was evaluated in articles which reported antibacterial activities of studied plants.<h4>Results</h4>Inhibition of bacterial growth at MIC of 256-1024 μg/mL was observed in only 15.4% of identical plant species. These values were 4-16-fold higher than those reported earlier. Further, 18.2% of related plant species had MICs of 128-256 μg/mL. Besides, 29.2% and 95.8% of the extracts were soluble to sparingly soluble in 10% DMSO and acetone, respectively. Extracts' solutions in both solvents showed similar qualitative compositions, with differing quantities of corresponding phytochemicals. Details regarding seasons and growth state at collection were missing in 65% and 95% of evaluated articles, respectively. Likewise, solvents used to dissolve the extracts were lacking in 30% of the articles, whereas 40% of them used unidentified bacterial isolates.<h4>Conclusion</h4>Reproducibility of previously reported activities from plants' extracts is a multi-factorial aspect. Thus, collective approaches are necessary in addressing the highlighted challenges.Nelson E MasotaGerd VoggKnut OhlsenUlrike HolzgrabePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255437 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nelson E Masota Gerd Vogg Knut Ohlsen Ulrike Holzgrabe Reproducibility challenges in the search for antibacterial compounds from nature.
description	<h4>Background</h4>Reproducibility of reported antibacterial activities of plant extracts has long remained questionable. Although plant-related factors should be well considered in serious pharmacognostic research, they are often not addressed in many research papers. Here we highlight the challenges in reproducing antibacterial activities of plant extracts.<h4>Methods</h4>Plants with reported antibacterial activities of interest were obtained from a literature review. Antibacterial activities against Escherichia coli and Klebsiella pneumoniae were tested using extracts' solutions in 10% DMSO and acetone. Compositions of working solutions from both solvents were established using LC-MS analysis. Moreover, the availability of details likely to affect reproducibility was evaluated in articles which reported antibacterial activities of studied plants.<h4>Results</h4>Inhibition of bacterial growth at MIC of 256-1024 μg/mL was observed in only 15.4% of identical plant species. These values were 4-16-fold higher than those reported earlier. Further, 18.2% of related plant species had MICs of 128-256 μg/mL. Besides, 29.2% and 95.8% of the extracts were soluble to sparingly soluble in 10% DMSO and acetone, respectively. Extracts' solutions in both solvents showed similar qualitative compositions, with differing quantities of corresponding phytochemicals. Details regarding seasons and growth state at collection were missing in 65% and 95% of evaluated articles, respectively. Likewise, solvents used to dissolve the extracts were lacking in 30% of the articles, whereas 40% of them used unidentified bacterial isolates.<h4>Conclusion</h4>Reproducibility of previously reported activities from plants' extracts is a multi-factorial aspect. Thus, collective approaches are necessary in addressing the highlighted challenges.
format	article
author	Nelson E Masota Gerd Vogg Knut Ohlsen Ulrike Holzgrabe
author_facet	Nelson E Masota Gerd Vogg Knut Ohlsen Ulrike Holzgrabe
author_sort	Nelson E Masota
title	Reproducibility challenges in the search for antibacterial compounds from nature.
title_short	Reproducibility challenges in the search for antibacterial compounds from nature.
title_full	Reproducibility challenges in the search for antibacterial compounds from nature.
title_fullStr	Reproducibility challenges in the search for antibacterial compounds from nature.
title_full_unstemmed	Reproducibility challenges in the search for antibacterial compounds from nature.
title_sort	reproducibility challenges in the search for antibacterial compounds from nature.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f8372c036c474dfc88c32b3c8ddcec36
work_keys_str_mv	AT nelsonemasota reproducibilitychallengesinthesearchforantibacterialcompoundsfromnature AT gerdvogg reproducibilitychallengesinthesearchforantibacterialcompoundsfromnature AT knutohlsen reproducibilitychallengesinthesearchforantibacterialcompoundsfromnature AT ulrikeholzgrabe reproducibilitychallengesinthesearchforantibacterialcompoundsfromnature
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Reproducibility challenges in the search for antibacterial compounds from nature.

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