Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb

Abstract Nanoparticulate matter activates the aryl hydrocarbon receptor (AhR) pathway in the respiratory system in a process involving the AhR nuclear translocator (ARNT) and cytochrome P450 family 1, member A1 (CYP1A1). We examined changes in AhR-related pathways following intranasal instillation o...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: So Young Kim, Kyung Woon Kim, So Min Lee, Sohyeon Park, Byeong-Gon Kim, Eun-kyung Choi, Bu Soon Son, Moo Kyun Park
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/f838731d74454ab5be2db3011995ab12
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:f838731d74454ab5be2db3011995ab12
record_format dspace
spelling oai:doaj.org-article:f838731d74454ab5be2db3011995ab122021-12-02T15:10:54ZEffects of intranasal instillation of nanoparticulate matter in the olfactory bulb10.1038/s41598-021-96593-02045-2322https://doaj.org/article/f838731d74454ab5be2db3011995ab122021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96593-0https://doaj.org/toc/2045-2322Abstract Nanoparticulate matter activates the aryl hydrocarbon receptor (AhR) pathway in the respiratory system in a process involving the AhR nuclear translocator (ARNT) and cytochrome P450 family 1, member A1 (CYP1A1). We examined changes in AhR-related pathways following intranasal instillation of nanoparticulate matter in the olfactory bulb and cerebral cortex. Twice a day for 5 days per week for 1 week or 2 weeks, 8-week-old Sprague–Dawley rats were intranasally instilled with 10 µL nanoparticulate matter (nano group; n = 36). An equal volume of saline was intranasally instilled in control rats (n = 36). One week after intranasal instillation, olfactory function and Y-maze tests were performed. The expression levels of AhR in the olfactory bulb and temporal cortex were analyzed using western blotting and immunofluorescence assays. The expression levels of AhR, CYP1A1, inducible nitric oxide synthase (iNOS), and five genes encoding cation transporters (ARNT, ATP7B, ATPB1, OCT1, and OCT2) in the olfactory bulb were analyzed using quantitative reverse transcription. The olfactory discrimination capability was reduced in the nano group compared with the control group. Proportional changes in the Y-maze test were not significantly different between the nano and control groups. AhR mRNA and protein expression in the olfactory bulb increased 1.71-fold (P < 0.001) and 1.60-fold (P = 0.008), respectively. However, no significant changes were observed in the temporal cortex. In the olfactory bulb, the expression of ARNT, ATP7B, ATPB1, and OCT2 was downregulated. CYP1A1 and iNOS expression in the olfactory bulb was upregulated compared with that in the temporal cortex. The intranasal instillation of nanoparticulate matter decreased the olfactory discrimination ability, which was accompanied by upregulation of AhR expression and downregulation of cation transporters in the olfactory bulb.So Young KimKyung Woon KimSo Min LeeSohyeon ParkByeong-Gon KimEun-kyung ChoiBu Soon SonMoo Kyun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
So Young Kim
Kyung Woon Kim
So Min Lee
Sohyeon Park
Byeong-Gon Kim
Eun-kyung Choi
Bu Soon Son
Moo Kyun Park
Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
description Abstract Nanoparticulate matter activates the aryl hydrocarbon receptor (AhR) pathway in the respiratory system in a process involving the AhR nuclear translocator (ARNT) and cytochrome P450 family 1, member A1 (CYP1A1). We examined changes in AhR-related pathways following intranasal instillation of nanoparticulate matter in the olfactory bulb and cerebral cortex. Twice a day for 5 days per week for 1 week or 2 weeks, 8-week-old Sprague–Dawley rats were intranasally instilled with 10 µL nanoparticulate matter (nano group; n = 36). An equal volume of saline was intranasally instilled in control rats (n = 36). One week after intranasal instillation, olfactory function and Y-maze tests were performed. The expression levels of AhR in the olfactory bulb and temporal cortex were analyzed using western blotting and immunofluorescence assays. The expression levels of AhR, CYP1A1, inducible nitric oxide synthase (iNOS), and five genes encoding cation transporters (ARNT, ATP7B, ATPB1, OCT1, and OCT2) in the olfactory bulb were analyzed using quantitative reverse transcription. The olfactory discrimination capability was reduced in the nano group compared with the control group. Proportional changes in the Y-maze test were not significantly different between the nano and control groups. AhR mRNA and protein expression in the olfactory bulb increased 1.71-fold (P < 0.001) and 1.60-fold (P = 0.008), respectively. However, no significant changes were observed in the temporal cortex. In the olfactory bulb, the expression of ARNT, ATP7B, ATPB1, and OCT2 was downregulated. CYP1A1 and iNOS expression in the olfactory bulb was upregulated compared with that in the temporal cortex. The intranasal instillation of nanoparticulate matter decreased the olfactory discrimination ability, which was accompanied by upregulation of AhR expression and downregulation of cation transporters in the olfactory bulb.
format article
author So Young Kim
Kyung Woon Kim
So Min Lee
Sohyeon Park
Byeong-Gon Kim
Eun-kyung Choi
Bu Soon Son
Moo Kyun Park
author_facet So Young Kim
Kyung Woon Kim
So Min Lee
Sohyeon Park
Byeong-Gon Kim
Eun-kyung Choi
Bu Soon Son
Moo Kyun Park
author_sort So Young Kim
title Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
title_short Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
title_full Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
title_fullStr Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
title_full_unstemmed Effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
title_sort effects of intranasal instillation of nanoparticulate matter in the olfactory bulb
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/f838731d74454ab5be2db3011995ab12
work_keys_str_mv AT soyoungkim effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT kyungwoonkim effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT sominlee effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT sohyeonpark effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT byeonggonkim effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT eunkyungchoi effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT busoonson effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
AT mookyunpark effectsofintranasalinstillationofnanoparticulatematterintheolfactorybulb
_version_ 1718387668071481344