Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA

Abstract Total choline (tCho) was documented as a biomarker for breast cancer diagnosis by in vivo MRS. To understand the molecular mechanisms behind elevated tCho in breast cancer, an association of tCho with β-catenin and cyclin D1 was evaluated. Hundred fractions from 20 malignant, 10 benign and...

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Autores principales:	Khushbu Agarwal, Gururao Hariprasad, Komal Rani, Uma Sharma, Sandeep R. Mathur, Vurthaluru Seenu, Rajinder Parshad, Naranamangalam R. Jagannathan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8393aaccd7749fd83863eec64487975
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spelling	oai:doaj.org-article:f8393aaccd7749fd83863eec644879752021-12-02T11:53:13ZIs there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA10.1038/s41598-017-01459-z2045-2322https://doaj.org/article/f8393aaccd7749fd83863eec644879752017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01459-zhttps://doaj.org/toc/2045-2322Abstract Total choline (tCho) was documented as a biomarker for breast cancer diagnosis by in vivo MRS. To understand the molecular mechanisms behind elevated tCho in breast cancer, an association of tCho with β-catenin and cyclin D1 was evaluated. Hundred fractions from 20 malignant, 10 benign and 20 non-involved breast tissues were isolated. Cytosolic and nuclear expressions of β-catenin and cyclin D1 were estimated using ELISA. Higher tCho was seen in malignant compared to benign tissues. Malignant tissues showed higher cytosolic and nuclear β-catenin expressions than benign and non-involved tissues. Within malignant tissues, β-catenin and cyclin D1 expressions were higher in the nucleus than cytosol. Cyclin D1 expression was higher in the cytosolic fractions of benign and non-involved than malignant tissues. Furthermore, in malignant tissues, tCho showed a positive correlation with the cytosolic and nuclear expression of β-catenin and cyclin D1 and also a correlation between nuclear expressions of both these proteins was seen. Higher cytosolic β-catenin expression was seen in progesterone receptor negative than positive patients. Results provide an evidence of correlation between non-invasive biomarker, tCho and the Wnt/β-catenin pathway. The findings explain the molecular mechanism of tCho elevation which may facilitate exploration of additional therapeutic targets for breast cancer.Khushbu AgarwalGururao HariprasadKomal RaniUma SharmaSandeep R. MathurVurthaluru SeenuRajinder ParshadNaranamangalam R. JagannathanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khushbu Agarwal Gururao Hariprasad Komal Rani Uma Sharma Sandeep R. Mathur Vurthaluru Seenu Rajinder Parshad Naranamangalam R. Jagannathan Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA
description	Abstract Total choline (tCho) was documented as a biomarker for breast cancer diagnosis by in vivo MRS. To understand the molecular mechanisms behind elevated tCho in breast cancer, an association of tCho with β-catenin and cyclin D1 was evaluated. Hundred fractions from 20 malignant, 10 benign and 20 non-involved breast tissues were isolated. Cytosolic and nuclear expressions of β-catenin and cyclin D1 were estimated using ELISA. Higher tCho was seen in malignant compared to benign tissues. Malignant tissues showed higher cytosolic and nuclear β-catenin expressions than benign and non-involved tissues. Within malignant tissues, β-catenin and cyclin D1 expressions were higher in the nucleus than cytosol. Cyclin D1 expression was higher in the cytosolic fractions of benign and non-involved than malignant tissues. Furthermore, in malignant tissues, tCho showed a positive correlation with the cytosolic and nuclear expression of β-catenin and cyclin D1 and also a correlation between nuclear expressions of both these proteins was seen. Higher cytosolic β-catenin expression was seen in progesterone receptor negative than positive patients. Results provide an evidence of correlation between non-invasive biomarker, tCho and the Wnt/β-catenin pathway. The findings explain the molecular mechanism of tCho elevation which may facilitate exploration of additional therapeutic targets for breast cancer.
format	article
author	Khushbu Agarwal Gururao Hariprasad Komal Rani Uma Sharma Sandeep R. Mathur Vurthaluru Seenu Rajinder Parshad Naranamangalam R. Jagannathan
author_facet	Khushbu Agarwal Gururao Hariprasad Komal Rani Uma Sharma Sandeep R. Mathur Vurthaluru Seenu Rajinder Parshad Naranamangalam R. Jagannathan
author_sort	Khushbu Agarwal
title	Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA
title_short	Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA
title_full	Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA
title_fullStr	Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA
title_full_unstemmed	Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA
title_sort	is there an association between enhanced choline and β-catenin pathway in breast cancer? a pilot study by mr spectroscopy and elisa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f8393aaccd7749fd83863eec64487975
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Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA

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