Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility

Islam Ali Abdel Raheem,1 Amro Abdul Razek,1 Abeer Abdelaziz Elgendy,1 Doaa Ahmed Labah,2 Noha Mohamed Saleh3 1Endodontics Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; 2Oral Biology and Dental Medicine Department, Faculty of Dentistry, Zagazig University, Zagazig, Egypt; 3P...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abdel Raheem IA, Abdul Razek A, Elgendy AA, Labah DA, Saleh NM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	propolis nanoparticles root sealer sealing ability dye extraction biocompatibility subcutaneous implantation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f8438e4554e34c6d8d8dc23577390b43
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f8438e4554e34c6d8d8dc23577390b43
record_format	dspace
spelling	oai:doaj.org-article:f8438e4554e34c6d8d8dc23577390b432021-12-02T08:42:10ZEgyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility1178-2013https://doaj.org/article/f8438e4554e34c6d8d8dc23577390b432020-07-01T00:00:00Zhttps://www.dovepress.com/egyptian-propolis-loaded-nanoparticles-as-a-root-canal-nanosealer-seal-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Islam Ali Abdel Raheem,1 Amro Abdul Razek,1 Abeer Abdelaziz Elgendy,1 Doaa Ahmed Labah,2 Noha Mohamed Saleh3 1Endodontics Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; 2Oral Biology and Dental Medicine Department, Faculty of Dentistry, Zagazig University, Zagazig, Egypt; 3Pharmaceutics Department, Faculty of Pharmacy, Mansoura University, Mansoura, EgyptCorrespondence: Noha Mohamed Saleh Email nunu_ramy@yahoo.comBackground: Successful endodontic therapy is mainly governed by the satisfactory sealing ability of the applied root canal sealer. Also, tolerability of root canal structure to accommodate the presence of a sealer participates in the efficiency of the treatment. Hence, this study was aimed to extrapolate our previous one that was concerned with the preparation and evaluation of novel nature-based root canal sealers. Our current work is focused on the evaluation of sealing ability and in vivo biocompatibility.Materials and Methods: Egyptian propolis was extracted (ProE) and encapsulated in polymeric nanoparticles (ProE-loaded NPs). Two root sealers, PE sealer and PE nanosealer, were fabricated by incorporating ProE and ProE-loaded NPs, respectively. The sealing ability of the developed sealers was tested by a dye extraction method. An in vivo biocompatibility study was conducted using a subcutaneous implantation method for two and four weeks. At the same time, a model sealer (AH Plus®) was subjected to the same procedures to enable accurate and equitable results.Results: The teeth treated with PE sealer exhibited weak sealing ability which did not differ from that of unfilled teeth. PE nanosealer enhanced the sealing ability similarly to the model sealer with minimal apical microleakage. Studying in vivo biocompatibility indicated the capability of the three tested sealers to induce cell proliferation and tissue healing. However, PE nanosealer had superior biocompatibility, with higher potential for cell regeneration and tissue proliferation.Conclusion: PE nanosealer can be presented as an innovative root canal sealer, with enhanced sealing ability as well as in vivo biocompatibility. It can be applied as a substitute for the currently available sealers that demonstrate hazardous effects.Keywords: propolis, nanoparticles, root sealer, sealing ability, dye extraction, biocompatibility, subcutaneous implantationAbdel Raheem IAAbdul Razek AElgendy AALabah DASaleh NMDove Medical Pressarticlepropolisnanoparticlesroot sealersealing abilitydye extractionbiocompatibilitysubcutaneous implantationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5265-5277 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	propolis nanoparticles root sealer sealing ability dye extraction biocompatibility subcutaneous implantation Medicine (General) R5-920
spellingShingle	propolis nanoparticles root sealer sealing ability dye extraction biocompatibility subcutaneous implantation Medicine (General) R5-920 Abdel Raheem IA Abdul Razek A Elgendy AA Labah DA Saleh NM Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility
description	Islam Ali Abdel Raheem,1 Amro Abdul Razek,1 Abeer Abdelaziz Elgendy,1 Doaa Ahmed Labah,2 Noha Mohamed Saleh3 1Endodontics Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt; 2Oral Biology and Dental Medicine Department, Faculty of Dentistry, Zagazig University, Zagazig, Egypt; 3Pharmaceutics Department, Faculty of Pharmacy, Mansoura University, Mansoura, EgyptCorrespondence: Noha Mohamed Saleh Email nunu_ramy@yahoo.comBackground: Successful endodontic therapy is mainly governed by the satisfactory sealing ability of the applied root canal sealer. Also, tolerability of root canal structure to accommodate the presence of a sealer participates in the efficiency of the treatment. Hence, this study was aimed to extrapolate our previous one that was concerned with the preparation and evaluation of novel nature-based root canal sealers. Our current work is focused on the evaluation of sealing ability and in vivo biocompatibility.Materials and Methods: Egyptian propolis was extracted (ProE) and encapsulated in polymeric nanoparticles (ProE-loaded NPs). Two root sealers, PE sealer and PE nanosealer, were fabricated by incorporating ProE and ProE-loaded NPs, respectively. The sealing ability of the developed sealers was tested by a dye extraction method. An in vivo biocompatibility study was conducted using a subcutaneous implantation method for two and four weeks. At the same time, a model sealer (AH Plus®) was subjected to the same procedures to enable accurate and equitable results.Results: The teeth treated with PE sealer exhibited weak sealing ability which did not differ from that of unfilled teeth. PE nanosealer enhanced the sealing ability similarly to the model sealer with minimal apical microleakage. Studying in vivo biocompatibility indicated the capability of the three tested sealers to induce cell proliferation and tissue healing. However, PE nanosealer had superior biocompatibility, with higher potential for cell regeneration and tissue proliferation.Conclusion: PE nanosealer can be presented as an innovative root canal sealer, with enhanced sealing ability as well as in vivo biocompatibility. It can be applied as a substitute for the currently available sealers that demonstrate hazardous effects.Keywords: propolis, nanoparticles, root sealer, sealing ability, dye extraction, biocompatibility, subcutaneous implantation
format	article
author	Abdel Raheem IA Abdul Razek A Elgendy AA Labah DA Saleh NM
author_facet	Abdel Raheem IA Abdul Razek A Elgendy AA Labah DA Saleh NM
author_sort	Abdel Raheem IA
title	Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility
title_short	Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility
title_full	Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility
title_fullStr	Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility
title_full_unstemmed	Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility
title_sort	egyptian propolis-loaded nanoparticles as a root canal nanosealer: sealing ability and in vivo biocompatibility
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/f8438e4554e34c6d8d8dc23577390b43
work_keys_str_mv	AT abdelraheemia egyptianpropolisloadednanoparticlesasarootcanalnanosealersealingabilityandinvivobiocompatibility AT abdulrazeka egyptianpropolisloadednanoparticlesasarootcanalnanosealersealingabilityandinvivobiocompatibility AT elgendyaa egyptianpropolisloadednanoparticlesasarootcanalnanosealersealingabilityandinvivobiocompatibility AT labahda egyptianpropolisloadednanoparticlesasarootcanalnanosealersealingabilityandinvivobiocompatibility AT salehnm egyptianpropolisloadednanoparticlesasarootcanalnanosealersealingabilityandinvivobiocompatibility
_version_	1718398441535569920

Egyptian Propolis-Loaded Nanoparticles as a Root Canal Nanosealer: Sealing Ability and in vivo Biocompatibility

Ejemplares similares