Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system

Abstract Porhyromonas gingivalis, a causative bacterium of periodontitis, is implicated in the etiology of rheumatoid arthritis (RA), mainly because of expressing peptidyl arginine deiminase (PAD) that generates RA-related autoantigens. However, compared with other periodontopathic bacteria, the pre...

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Autores principales:	Keisuke Sato, Naoki Takahashi, Tamotsu Kato, Yumi Matsuda, Mai Yokoji, Miki Yamada, Takako Nakajima, Naoki Kondo, Naoto Endo, Reiko Yamamoto, Yuichiro Noiri, Hiroshi Ohno, Kazuhisa Yamazaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f84459cd247f4b6fb9cb1fd2ed03ea80
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spelling	oai:doaj.org-article:f84459cd247f4b6fb9cb1fd2ed03ea802021-12-02T15:06:00ZAggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system10.1038/s41598-017-07196-72045-2322https://doaj.org/article/f84459cd247f4b6fb9cb1fd2ed03ea802017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07196-7https://doaj.org/toc/2045-2322Abstract Porhyromonas gingivalis, a causative bacterium of periodontitis, is implicated in the etiology of rheumatoid arthritis (RA), mainly because of expressing peptidyl arginine deiminase (PAD) that generates RA-related autoantigens. However, compared with other periodontopathic bacteria, the precise role of P. gingivalis in RA is largely unknown. We found that orally administered P. gingivalis changed the gut microbiome with concomitant elevation of serum endotoxin and inflammatory markers, and impairment of the gut barrier function. Based on findings showing a relationship between gut microbiota and RA, we investigated whether the change of gut microbiota induced by P. gingivalis and Prevotella intermedia, another periodontopathic bacterium without PAD, is associated with collagen-induced arthritis (CIA). DBA/1J mice were orally administered with or without bacteria followed by induction of CIA. P. gingivalis, but not P. intermedia, administration significantly aggravated arthritis with increased interleukin-17 levels in sera and culture supernatants, increased Th17 cell proportions among mesenteric lymphocytes, and a significant change in the gut microbiome. However, P. gingivalis administration did not elevate the level of anti-citrullinated protein antibody. These results suggest a unique role of P. gingivalis in the link between periodontitis and RA by affecting the gut immune system and the gut microbiota composition.Keisuke SatoNaoki TakahashiTamotsu KatoYumi MatsudaMai YokojiMiki YamadaTakako NakajimaNaoki KondoNaoto EndoReiko YamamotoYuichiro NoiriHiroshi OhnoKazuhisa YamazakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keisuke Sato Naoki Takahashi Tamotsu Kato Yumi Matsuda Mai Yokoji Miki Yamada Takako Nakajima Naoki Kondo Naoto Endo Reiko Yamamoto Yuichiro Noiri Hiroshi Ohno Kazuhisa Yamazaki Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
description	Abstract Porhyromonas gingivalis, a causative bacterium of periodontitis, is implicated in the etiology of rheumatoid arthritis (RA), mainly because of expressing peptidyl arginine deiminase (PAD) that generates RA-related autoantigens. However, compared with other periodontopathic bacteria, the precise role of P. gingivalis in RA is largely unknown. We found that orally administered P. gingivalis changed the gut microbiome with concomitant elevation of serum endotoxin and inflammatory markers, and impairment of the gut barrier function. Based on findings showing a relationship between gut microbiota and RA, we investigated whether the change of gut microbiota induced by P. gingivalis and Prevotella intermedia, another periodontopathic bacterium without PAD, is associated with collagen-induced arthritis (CIA). DBA/1J mice were orally administered with or without bacteria followed by induction of CIA. P. gingivalis, but not P. intermedia, administration significantly aggravated arthritis with increased interleukin-17 levels in sera and culture supernatants, increased Th17 cell proportions among mesenteric lymphocytes, and a significant change in the gut microbiome. However, P. gingivalis administration did not elevate the level of anti-citrullinated protein antibody. These results suggest a unique role of P. gingivalis in the link between periodontitis and RA by affecting the gut immune system and the gut microbiota composition.
format	article
author	Keisuke Sato Naoki Takahashi Tamotsu Kato Yumi Matsuda Mai Yokoji Miki Yamada Takako Nakajima Naoki Kondo Naoto Endo Reiko Yamamoto Yuichiro Noiri Hiroshi Ohno Kazuhisa Yamazaki
author_facet	Keisuke Sato Naoki Takahashi Tamotsu Kato Yumi Matsuda Mai Yokoji Miki Yamada Takako Nakajima Naoki Kondo Naoto Endo Reiko Yamamoto Yuichiro Noiri Hiroshi Ohno Kazuhisa Yamazaki
author_sort	Keisuke Sato
title	Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
title_short	Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
title_full	Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
title_fullStr	Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
title_full_unstemmed	Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
title_sort	aggravation of collagen-induced arthritis by orally administered porphyromonas gingivalis through modulation of the gut microbiota and gut immune system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f84459cd247f4b6fb9cb1fd2ed03ea80
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Aggravation of collagen-induced arthritis by orally administered Porphyromonas gingivalis through modulation of the gut microbiota and gut immune system

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