Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.

Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions s...

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Autores principales:	Jingjing Zhai, Limin Wang, Xiaoliang Qiao, Jianping Zhao, Xuexia Wang, Xiaohong He
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8518ea7a19c41fa93b2886c2633269d
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spelling	oai:doaj.org-article:f8518ea7a19c41fa93b2886c2633269d2021-11-25T06:19:20ZDetection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.1932-620310.1371/journal.pone.0251119https://doaj.org/article/f8518ea7a19c41fa93b2886c2633269d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251119https://doaj.org/toc/1932-6203Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions such as the ratio of primer concentration, magnesium ion concentration, amplification time and template DNA concentration in the multiplex RPA reaction were optimized. The optimized multiplex RPA-LFD method was used to detect both CT and NG positive control plasmids, and it was found that the LFD could be used to obtain visible results when the plasmid copy number was only 200. The sensitivity of the multiplex RPA-LFD method used for clinical samples was 85.62 (95% CI at 53.66-97.29) for NG detection and 90.90 (95% CI at 57.12-99.52) for CT detection.Jingjing ZhaiLimin WangXiaoliang QiaoJianping ZhaoXuexia WangXiaohong HePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251119 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jingjing Zhai Limin Wang Xiaoliang Qiao Jianping Zhao Xuexia Wang Xiaohong He Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
description	Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions such as the ratio of primer concentration, magnesium ion concentration, amplification time and template DNA concentration in the multiplex RPA reaction were optimized. The optimized multiplex RPA-LFD method was used to detect both CT and NG positive control plasmids, and it was found that the LFD could be used to obtain visible results when the plasmid copy number was only 200. The sensitivity of the multiplex RPA-LFD method used for clinical samples was 85.62 (95% CI at 53.66-97.29) for NG detection and 90.90 (95% CI at 57.12-99.52) for CT detection.
format	article
author	Jingjing Zhai Limin Wang Xiaoliang Qiao Jianping Zhao Xuexia Wang Xiaohong He
author_facet	Jingjing Zhai Limin Wang Xiaoliang Qiao Jianping Zhao Xuexia Wang Xiaohong He
author_sort	Jingjing Zhai
title	Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
title_short	Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
title_full	Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
title_fullStr	Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
title_full_unstemmed	Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
title_sort	detection of neisseria gonorrhoeae and chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f8518ea7a19c41fa93b2886c2633269d
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Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.

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