High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect

Shan Liu,1,2 Wei Huang,1,2 Ming-Ji Jin,1,2 Qi-Ming Wang,1,2 Gan-Lin Zhang,3 Xiao-Min Wang,3 Shuai Shao,1,2 Zhong-Gao Gao1,2 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 2Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences...

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Autores principales:	Liu S, Huang W, Jin MJ, Wang QM, Zhang GL, Wang XM, Shao S, Gao ZG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f855c564d5b34776a98626db06a4d9fb
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spelling	oai:doaj.org-article:f855c564d5b34776a98626db06a4d9fb2021-12-02T06:47:18ZHigh gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect1178-2013https://doaj.org/article/f855c564d5b34776a98626db06a4d9fb2014-07-01T00:00:00Zhttp://www.dovepress.com/high-gene-delivery-efficiency-of-alkylated-low-molecular-weight-polyet-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Shan Liu,1,2 Wei Huang,1,2 Ming-Ji Jin,1,2 Qi-Ming Wang,1,2 Gan-Lin Zhang,3 Xiao-Min Wang,3 Shuai Shao,1,2 Zhong-Gao Gao1,2 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 2Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China; 3Beijing Hospital of Traditional Chinese Medicine affiliated to Capital Medical University, Beijing, People’s Republic of China Abstract: To our knowledge, the mechanism underlying the high transfection efficiency of alkylated low-molecular-weight polyethylenimine (PEI) is not yet well understood. In this work, we grafted branched PEI (molecular weight of 1,800 Da; bPEI1800) with lauryl chains (C12), and found that bPEI1800-C12 was structurally similar to gemini surfactant and could similarly assemble into micelle-like particles. Stability, cellular uptake, and lysosome escape ability of bPEI1800-C12/DNA polyplexes were all greatly enhanced after C12 grafting. bPEI1800-C12/DNA polyplexes exhibited significantly higher transfection efficiency than Lipofectamine™ 2000 in the presence of serum. Bioluminescence imaging showed that systemic injection of bPEI1800-C12/DNA polyplexes resulted in intensive luciferase expression in vivo and bioluminescence signals that could be detected even in the head. Altogether, the high transfection efficacy of bPEI1800-C12 was because bPEI1800-C12, being an analog of gemini surfactant, facilitated lysosome escape and induced the coil–globule transition of DNA to assemble into a highly organized micelle-like structure that showed high stability. Keywords: self-organization, alkylation, luciferase, bioluminescence imagingLiu SHuang WJin MJWang QMZhang GLWang XMShao SGao ZGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3567-3581 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Liu S Huang W Jin MJ Wang QM Zhang GL Wang XM Shao S Gao ZG High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
description	Shan Liu,1,2 Wei Huang,1,2 Ming-Ji Jin,1,2 Qi-Ming Wang,1,2 Gan-Lin Zhang,3 Xiao-Min Wang,3 Shuai Shao,1,2 Zhong-Gao Gao1,2 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, 2Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China; 3Beijing Hospital of Traditional Chinese Medicine affiliated to Capital Medical University, Beijing, People’s Republic of China Abstract: To our knowledge, the mechanism underlying the high transfection efficiency of alkylated low-molecular-weight polyethylenimine (PEI) is not yet well understood. In this work, we grafted branched PEI (molecular weight of 1,800 Da; bPEI1800) with lauryl chains (C12), and found that bPEI1800-C12 was structurally similar to gemini surfactant and could similarly assemble into micelle-like particles. Stability, cellular uptake, and lysosome escape ability of bPEI1800-C12/DNA polyplexes were all greatly enhanced after C12 grafting. bPEI1800-C12/DNA polyplexes exhibited significantly higher transfection efficiency than Lipofectamine™ 2000 in the presence of serum. Bioluminescence imaging showed that systemic injection of bPEI1800-C12/DNA polyplexes resulted in intensive luciferase expression in vivo and bioluminescence signals that could be detected even in the head. Altogether, the high transfection efficacy of bPEI1800-C12 was because bPEI1800-C12, being an analog of gemini surfactant, facilitated lysosome escape and induced the coil–globule transition of DNA to assemble into a highly organized micelle-like structure that showed high stability. Keywords: self-organization, alkylation, luciferase, bioluminescence imaging
format	article
author	Liu S Huang W Jin MJ Wang QM Zhang GL Wang XM Shao S Gao ZG
author_facet	Liu S Huang W Jin MJ Wang QM Zhang GL Wang XM Shao S Gao ZG
author_sort	Liu S
title	High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
title_short	High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
title_full	High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
title_fullStr	High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
title_full_unstemmed	High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
title_sort	high gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/f855c564d5b34776a98626db06a4d9fb
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High gene delivery efficiency of alkylated low-molecular-weight polyethylenimine through gemini surfactant-like effect

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