INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS

Abstract. We demonstrated that TNFα, IFNγ and IL-4 induce different patterns of endothelial cells activation. The greatest alterations were observed in TNFα threated endothelial cells. Under the influence of this cytokine there was a statistically significant elevation of IL-8 and MCP-1 production b...

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Autores principales:	E. A. Starikova, I. S. Freidlin, D. I. Sokolov, S. A. Selkov, K. A. Sisoev, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	endothelial cells cytokines monocytes adhesion migration phenotype Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f8567dde2b1c4fcfaa031138a9c3d1dd
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spelling	oai:doaj.org-article:f8567dde2b1c4fcfaa031138a9c3d1dd2021-11-18T08:03:43ZINFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS1563-06252313-741X10.15789/1563-0625-2005-5-6-495-502https://doaj.org/article/f8567dde2b1c4fcfaa031138a9c3d1dd2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/593https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. We demonstrated that TNFα, IFNγ and IL-4 induce different patterns of endothelial cells activation. The greatest alterations were observed in TNFα threated endothelial cells. Under the influence of this cytokine there was a statistically significant elevation of IL-8 and MCP-1 production by endothelial cells, of THP-1 adhesion and of migration toward endothelial cells monolayer. At the same time we had not observed any immunophenotype alteration of THP-1 cocultured with endothelial cells treated with TNFα.IFNγ и IL-4 induced statistically significant inhibition of THP-1 adhesion. IL-4 treated endothelial cells have elevated levels of IL-8 and MCP-1 production and stimulated THP-1 migrations. IFNγ showed inhibition of IL-8 and MCP-1 spontaneous production and THP-1migration.Incubation of THP-1 with untreated and IFNγ treated endothelial cells led to significant elevation of HLA-DR expression. Expression of CD68 was significantly higher in case of THP-1 cocultured with IL-4 treated endothelial cells. There was no any correlation between immunophenotype alteration THP-1cells and others parameters alteration.E. A. StarikovaI. S. FreidlinD. I. SokolovS. A. SelkovK. A. SisoevAreg A. TotolianSPb RAACIarticleendothelial cellscytokinesmonocytesadhesionmigrationphenotypeImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 7, Iss 5-6, Pp 495-502 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	endothelial cells cytokines monocytes adhesion migration phenotype Immunologic diseases. Allergy RC581-607
spellingShingle	endothelial cells cytokines monocytes adhesion migration phenotype Immunologic diseases. Allergy RC581-607 E. A. Starikova I. S. Freidlin D. I. Sokolov S. A. Selkov K. A. Sisoev Areg A. Totolian INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS
description	Abstract. We demonstrated that TNFα, IFNγ and IL-4 induce different patterns of endothelial cells activation. The greatest alterations were observed in TNFα threated endothelial cells. Under the influence of this cytokine there was a statistically significant elevation of IL-8 and MCP-1 production by endothelial cells, of THP-1 adhesion and of migration toward endothelial cells monolayer. At the same time we had not observed any immunophenotype alteration of THP-1 cocultured with endothelial cells treated with TNFα.IFNγ и IL-4 induced statistically significant inhibition of THP-1 adhesion. IL-4 treated endothelial cells have elevated levels of IL-8 and MCP-1 production and stimulated THP-1 migrations. IFNγ showed inhibition of IL-8 and MCP-1 spontaneous production and THP-1migration.Incubation of THP-1 with untreated and IFNγ treated endothelial cells led to significant elevation of HLA-DR expression. Expression of CD68 was significantly higher in case of THP-1 cocultured with IL-4 treated endothelial cells. There was no any correlation between immunophenotype alteration THP-1cells and others parameters alteration.
format	article
author	E. A. Starikova I. S. Freidlin D. I. Sokolov S. A. Selkov K. A. Sisoev Areg A. Totolian
author_facet	E. A. Starikova I. S. Freidlin D. I. Sokolov S. A. Selkov K. A. Sisoev Areg A. Totolian
author_sort	E. A. Starikova
title	INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS
title_short	INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS
title_full	INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS
title_fullStr	INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS
title_full_unstemmed	INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS
title_sort	influence of endothelial cells preincubated with cytokines on thp-1 monocyte-like cells phenotype and functions
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/f8567dde2b1c4fcfaa031138a9c3d1dd
work_keys_str_mv	AT eastarikova influenceofendothelialcellspreincubatedwithcytokinesonthp1monocytelikecellsphenotypeandfunctions AT isfreidlin influenceofendothelialcellspreincubatedwithcytokinesonthp1monocytelikecellsphenotypeandfunctions AT disokolov influenceofendothelialcellspreincubatedwithcytokinesonthp1monocytelikecellsphenotypeandfunctions AT saselkov influenceofendothelialcellspreincubatedwithcytokinesonthp1monocytelikecellsphenotypeandfunctions AT kasisoev influenceofendothelialcellspreincubatedwithcytokinesonthp1monocytelikecellsphenotypeandfunctions AT aregatotolian influenceofendothelialcellspreincubatedwithcytokinesonthp1monocytelikecellsphenotypeandfunctions
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INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS

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