Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types

Background: Glioblastoma multiforme is the most aggressive astrocytoma in adults. Glioblastoma is a heterogenous tumor formed from various cells including astrocyte and cancer stem cells. Here, we explain the isolation, culture, morphology and specific markers of human glioblastoma astrocyte and ste...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Mansoureh Hashemi
Formato:	article
Lenguaje:	EN
Publicado:	Shahid Beheshti University of Medical Sciences 2020
Materias:	glioblastoma multiforme astrocyte cancer stem cell morphology Medicine R
Acceso en línea:	https://doaj.org/article/f856da7119bc4f13b3e7290be3a0fb47
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f856da7119bc4f13b3e7290be3a0fb47
record_format	dspace
spelling	oai:doaj.org-article:f856da7119bc4f13b3e7290be3a0fb472021-11-16T10:50:32ZIsolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types2383-18712383-209610.34172/icnj.2021.05https://doaj.org/article/f856da7119bc4f13b3e7290be3a0fb472020-12-01T00:00:00Zhttps://journals.sbmu.ac.ir/neuroscience/article/view/33554/26386https://doaj.org/toc/2383-1871https://doaj.org/toc/2383-2096Background: Glioblastoma multiforme is the most aggressive astrocytoma in adults. Glioblastoma is a heterogenous tumor formed from various cells including astrocyte and cancer stem cells. Here, we explain the isolation, culture, morphology and specific markers of human glioblastoma astrocyte and stem cells. Methods: We isolated astrocyte and cancer stem cells from human glioblastoma tissue. The obtained glioblastoma tissues were digested and cultured in DMEM12, B27 supplemented with basic fibroblast growth factor and epidermal growth factor. The morphology and specific markers were assessed in astrocyte and cancer stem cell of human glioblastoma through immunocytochemistry. Results: Results indicated that there were two morphology types in cell culture including epithelioid morphology and fibroblastic morphology. The astrocyte confirmed via expression of the Glial fibrillary acidic protein (GFAP) protein. Cancer stem cells were round and floating in the culture medium. Immunocytochemical staining indicated that nestin and SRY-box 2 (SOX2) antigens were positively expressed in primary neurospheres. Conclusion: The expression of glial and stem cell markers shows that both cells are in the human glioblastoma.Mansoureh HashemiShahid Beheshti University of Medical Sciencesarticleglioblastoma multiformeastrocytecancer stem cellmorphologyMedicineRENInternational Clinical Neuroscience Journal, Vol 8, Iss 1, Pp 22-25 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioblastoma multiforme astrocyte cancer stem cell morphology Medicine R
spellingShingle	glioblastoma multiforme astrocyte cancer stem cell morphology Medicine R Mansoureh Hashemi Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types
description	Background: Glioblastoma multiforme is the most aggressive astrocytoma in adults. Glioblastoma is a heterogenous tumor formed from various cells including astrocyte and cancer stem cells. Here, we explain the isolation, culture, morphology and specific markers of human glioblastoma astrocyte and stem cells. Methods: We isolated astrocyte and cancer stem cells from human glioblastoma tissue. The obtained glioblastoma tissues were digested and cultured in DMEM12, B27 supplemented with basic fibroblast growth factor and epidermal growth factor. The morphology and specific markers were assessed in astrocyte and cancer stem cell of human glioblastoma through immunocytochemistry. Results: Results indicated that there were two morphology types in cell culture including epithelioid morphology and fibroblastic morphology. The astrocyte confirmed via expression of the Glial fibrillary acidic protein (GFAP) protein. Cancer stem cells were round and floating in the culture medium. Immunocytochemical staining indicated that nestin and SRY-box 2 (SOX2) antigens were positively expressed in primary neurospheres. Conclusion: The expression of glial and stem cell markers shows that both cells are in the human glioblastoma.
format	article
author	Mansoureh Hashemi
author_facet	Mansoureh Hashemi
author_sort	Mansoureh Hashemi
title	Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types
title_short	Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types
title_full	Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types
title_fullStr	Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types
title_full_unstemmed	Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types
title_sort	isolation of cancer stem cells and astrocytes from human glioblastoma: morphological characterization of two cells types
publisher	Shahid Beheshti University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/f856da7119bc4f13b3e7290be3a0fb47
work_keys_str_mv	AT mansourehhashemi isolationofcancerstemcellsandastrocytesfromhumanglioblastomamorphologicalcharacterizationoftwocellstypes
_version_	1718426581967306752

Isolation of Cancer Stem Cells and Astrocytes from Human Glioblastoma: Morphological Characterization of Two Cells Types

Ejemplares similares