A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses

Abiotic stresses reduce crop growth and yield in part by disrupting metabolic homeostasis and triggering responses that change the metabolome. Experiments designed to understand the mechanisms underlying these metabolomic responses have usually not used agriculturally relevant stress regimes. We the...

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Autores principales:	Jaya Joshi, Ghulam Hasnain, Taylor Logue, Madeline Lynch, Shan Wu, Jiahn-Chou Guan, Saleh Alseekh, Alisdair R. Fernie, Andrew D. Hanson, Donald R. McCarty
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	drought salinity heat stress metabolomics RNA-seq Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f858d0c1a0bb48198c0514dc5ab7ad77
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spelling	oai:doaj.org-article:f858d0c1a0bb48198c0514dc5ab7ad772021-11-25T18:20:59ZA Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses10.3390/metabo111107972218-1989https://doaj.org/article/f858d0c1a0bb48198c0514dc5ab7ad772021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/797https://doaj.org/toc/2218-1989Abiotic stresses reduce crop growth and yield in part by disrupting metabolic homeostasis and triggering responses that change the metabolome. Experiments designed to understand the mechanisms underlying these metabolomic responses have usually not used agriculturally relevant stress regimes. We therefore subjected maize plants to drought, salt, or heat stresses that mimic field conditions and analyzed leaf responses at metabolome and transcriptome levels. Shared features of stress metabolomes included synthesis of raffinose, a compatible solute implicated in tolerance to dehydration. In addition, a marked accumulation of amino acids including proline, arginine, and γ-aminobutyrate combined with depletion of key glycolysis and tricarboxylic acid cycle intermediates indicated a shift in balance of carbon and nitrogen metabolism in stressed leaves. Involvement of the γ-aminobutyrate shunt in this process is consistent with its previously proposed role as a workaround for stress-induced thiamin-deficiency. Although convergent metabolome shifts were correlated with gene expression changes in affected pathways, patterns of differential gene regulation induced by the three stresses indicated distinct signaling mechanisms highlighting the plasticity of plant metabolic responses to abiotic stress.Jaya JoshiGhulam HasnainTaylor LogueMadeline LynchShan WuJiahn-Chou GuanSaleh AlseekhAlisdair R. FernieAndrew D. HansonDonald R. McCartyMDPI AGarticledroughtsalinityheat stressmetabolomicsRNA-seqMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 797, p 797 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drought salinity heat stress metabolomics RNA-seq Microbiology QR1-502
spellingShingle	drought salinity heat stress metabolomics RNA-seq Microbiology QR1-502 Jaya Joshi Ghulam Hasnain Taylor Logue Madeline Lynch Shan Wu Jiahn-Chou Guan Saleh Alseekh Alisdair R. Fernie Andrew D. Hanson Donald R. McCarty A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses
description	Abiotic stresses reduce crop growth and yield in part by disrupting metabolic homeostasis and triggering responses that change the metabolome. Experiments designed to understand the mechanisms underlying these metabolomic responses have usually not used agriculturally relevant stress regimes. We therefore subjected maize plants to drought, salt, or heat stresses that mimic field conditions and analyzed leaf responses at metabolome and transcriptome levels. Shared features of stress metabolomes included synthesis of raffinose, a compatible solute implicated in tolerance to dehydration. In addition, a marked accumulation of amino acids including proline, arginine, and γ-aminobutyrate combined with depletion of key glycolysis and tricarboxylic acid cycle intermediates indicated a shift in balance of carbon and nitrogen metabolism in stressed leaves. Involvement of the γ-aminobutyrate shunt in this process is consistent with its previously proposed role as a workaround for stress-induced thiamin-deficiency. Although convergent metabolome shifts were correlated with gene expression changes in affected pathways, patterns of differential gene regulation induced by the three stresses indicated distinct signaling mechanisms highlighting the plasticity of plant metabolic responses to abiotic stress.
format	article
author	Jaya Joshi Ghulam Hasnain Taylor Logue Madeline Lynch Shan Wu Jiahn-Chou Guan Saleh Alseekh Alisdair R. Fernie Andrew D. Hanson Donald R. McCarty
author_facet	Jaya Joshi Ghulam Hasnain Taylor Logue Madeline Lynch Shan Wu Jiahn-Chou Guan Saleh Alseekh Alisdair R. Fernie Andrew D. Hanson Donald R. McCarty
author_sort	Jaya Joshi
title	A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses
title_short	A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses
title_full	A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses
title_fullStr	A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses
title_full_unstemmed	A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses
title_sort	core metabolome response of maize leaves subjected to long-duration abiotic stresses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f858d0c1a0bb48198c0514dc5ab7ad77
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A Core Metabolome Response of Maize Leaves Subjected to Long-Duration Abiotic Stresses

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