Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.

Recently a first genome-wide analysis of translational regulation using prokaryotic species had been performed which revealed that regulation of translational efficiency plays an important role in haloarchaea. In fact, the fractions of genes under differential growth phase-dependent translational co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariam Brenneis, Jörg Soppa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f86407f7142b4f1a93fd904742ed8547
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f86407f7142b4f1a93fd904742ed8547
record_format	dspace
spelling	oai:doaj.org-article:f86407f7142b4f1a93fd904742ed85472021-11-25T06:17:19ZRegulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.1932-620310.1371/journal.pone.0004484https://doaj.org/article/f86407f7142b4f1a93fd904742ed85472009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19214227/?tool=EBIhttps://doaj.org/toc/1932-6203Recently a first genome-wide analysis of translational regulation using prokaryotic species had been performed which revealed that regulation of translational efficiency plays an important role in haloarchaea. In fact, the fractions of genes under differential growth phase-dependent translational control in the two species Halobacterium salinarum and Haloferax volcanii were as high as in eukaryotes. However, nothing is known about the mechanisms of translational regulation in archaea. Therefore, two genes exhibiting opposing directions of regulation were selected to unravel the importance of untranslated regions (UTRs) for differential translational control in vivo.Differential translational regulation in exponentially growing versus stationary phase cells was studied by comparing translational efficiencies using a reporter gene system. Translational regulation was not observed when 5'-UTRs or 3'-UTRs alone were fused to the reporter gene. However, their simultaneous presence was sufficient to transfer differential translational control from the native transcript to the reporter transcript. This was true for both directions of translational control. Translational regulation was completely abolished when stem loops in the 5'-UTR were changed by mutagenesis. An "UTR-swap" experiment demonstrated that the direction of translational regulation is encoded in the 3'-UTR, not in the 5'-UTR. While much is known about 5'-UTR-dependent translational control in bacteria, the reported findings provide the first examples that both 5'- and 3'-UTRs are essential and sufficient to drive differential translational regulation in a prokaryote and therefore have to functionally interact in vivo. The current results indicate that 3'-UTR-dependent translational control had already evolved before capping and polyadenylation of transcripts were invented, which are essential for circularization of transcripts in eukaryotes.Mariam BrenneisJörg SoppaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4484 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariam Brenneis Jörg Soppa Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.
description	Recently a first genome-wide analysis of translational regulation using prokaryotic species had been performed which revealed that regulation of translational efficiency plays an important role in haloarchaea. In fact, the fractions of genes under differential growth phase-dependent translational control in the two species Halobacterium salinarum and Haloferax volcanii were as high as in eukaryotes. However, nothing is known about the mechanisms of translational regulation in archaea. Therefore, two genes exhibiting opposing directions of regulation were selected to unravel the importance of untranslated regions (UTRs) for differential translational control in vivo.Differential translational regulation in exponentially growing versus stationary phase cells was studied by comparing translational efficiencies using a reporter gene system. Translational regulation was not observed when 5'-UTRs or 3'-UTRs alone were fused to the reporter gene. However, their simultaneous presence was sufficient to transfer differential translational control from the native transcript to the reporter transcript. This was true for both directions of translational control. Translational regulation was completely abolished when stem loops in the 5'-UTR were changed by mutagenesis. An "UTR-swap" experiment demonstrated that the direction of translational regulation is encoded in the 3'-UTR, not in the 5'-UTR. While much is known about 5'-UTR-dependent translational control in bacteria, the reported findings provide the first examples that both 5'- and 3'-UTRs are essential and sufficient to drive differential translational regulation in a prokaryote and therefore have to functionally interact in vivo. The current results indicate that 3'-UTR-dependent translational control had already evolved before capping and polyadenylation of transcripts were invented, which are essential for circularization of transcripts in eukaryotes.
format	article
author	Mariam Brenneis Jörg Soppa
author_facet	Mariam Brenneis Jörg Soppa
author_sort	Mariam Brenneis
title	Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.
title_short	Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.
title_full	Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.
title_fullStr	Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.
title_full_unstemmed	Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.
title_sort	regulation of translation in haloarchaea: 5'- and 3'-utrs are essential and have to functionally interact in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/f86407f7142b4f1a93fd904742ed8547
work_keys_str_mv	AT mariambrenneis regulationoftranslationinhaloarchaea5and3utrsareessentialandhavetofunctionallyinteractinvivo AT jorgsoppa regulationoftranslationinhaloarchaea5and3utrsareessentialandhavetofunctionallyinteractinvivo
_version_	1718414019772022784

Regulation of translation in haloarchaea: 5'- and 3'-UTRs are essential and have to functionally interact in vivo.

Ejemplares similares