Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.

The transformer (tra) gene is essential for female development in many insect species, including the Australian sheep blow fly, Lucilia cuprina. Sex-specific tra RNA splicing is controlled by Sex lethal (Sxl) in Drosophila melanogaster but is auto-regulated in L. cuprina. Sxl also represses X chromo...

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Autores principales:	Megan E Williamson, Ying Yan, Maxwell J Scott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f8672dbaf8d14991bb6b0cc2b484a2cb
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spelling	oai:doaj.org-article:f8672dbaf8d14991bb6b0cc2b484a2cb2021-12-02T20:03:31ZConditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.1553-73901553-740410.1371/journal.pgen.1009792https://doaj.org/article/f8672dbaf8d14991bb6b0cc2b484a2cb2021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009792https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The transformer (tra) gene is essential for female development in many insect species, including the Australian sheep blow fly, Lucilia cuprina. Sex-specific tra RNA splicing is controlled by Sex lethal (Sxl) in Drosophila melanogaster but is auto-regulated in L. cuprina. Sxl also represses X chromosome dosage compensation in female D. melanogaster. We have developed conditional Lctra RNAi knockdown strains using the tet-off system. Four strains did not produce females on diet without tetracycline and could potentially be used for genetic control of L. cuprina. In one strain, which showed both maternal and zygotic tTA expression, most XX transformed males died at the pupal stage. RNAseq and qRT-PCR analyses of mid-stage pupae showed increased expression of X-linked genes in XX individuals. These results suggest that Lctra promotes somatic sexual differentiation and inhibits X chromosome dosage compensation in female L. cuprina. However, XX flies homozygous for a loss-of-function Lctra knockin mutation were fully transformed and showed high pupal eclosion. Two of five X-linked genes examined showed a significant increase in mRNA levels in XX males. The stronger phenotype in the RNAi knockdown strain could indicate that maternal Lctra expression may be essential for initiation of dosage compensation suppression in female embryos.Megan E WilliamsonYing YanMaxwell J ScottPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 10, p e1009792 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Megan E Williamson Ying Yan Maxwell J Scott Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
description	The transformer (tra) gene is essential for female development in many insect species, including the Australian sheep blow fly, Lucilia cuprina. Sex-specific tra RNA splicing is controlled by Sex lethal (Sxl) in Drosophila melanogaster but is auto-regulated in L. cuprina. Sxl also represses X chromosome dosage compensation in female D. melanogaster. We have developed conditional Lctra RNAi knockdown strains using the tet-off system. Four strains did not produce females on diet without tetracycline and could potentially be used for genetic control of L. cuprina. In one strain, which showed both maternal and zygotic tTA expression, most XX transformed males died at the pupal stage. RNAseq and qRT-PCR analyses of mid-stage pupae showed increased expression of X-linked genes in XX individuals. These results suggest that Lctra promotes somatic sexual differentiation and inhibits X chromosome dosage compensation in female L. cuprina. However, XX flies homozygous for a loss-of-function Lctra knockin mutation were fully transformed and showed high pupal eclosion. Two of five X-linked genes examined showed a significant increase in mRNA levels in XX males. The stronger phenotype in the RNAi knockdown strain could indicate that maternal Lctra expression may be essential for initiation of dosage compensation suppression in female embryos.
format	article
author	Megan E Williamson Ying Yan Maxwell J Scott
author_facet	Megan E Williamson Ying Yan Maxwell J Scott
author_sort	Megan E Williamson
title	Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
title_short	Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
title_full	Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
title_fullStr	Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
title_full_unstemmed	Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
title_sort	conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f8672dbaf8d14991bb6b0cc2b484a2cb
work_keys_str_mv	AT meganewilliamson conditionalknockdownoftransformerinsheepblowflysuggestsaroleinrepressionofdosagecompensationandpotentialforpopulationsuppression AT yingyan conditionalknockdownoftransformerinsheepblowflysuggestsaroleinrepressionofdosagecompensationandpotentialforpopulationsuppression AT maxwelljscott conditionalknockdownoftransformerinsheepblowflysuggestsaroleinrepressionofdosagecompensationandpotentialforpopulationsuppression
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Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.

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