Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer

Abstract Mouse model induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) is generally accepted as an ideal object to study on the carcinogenesis mechanisms of human colorectal cancer (CRC). The genomic responses to the AOM/DSS treatment in mouse that possibly lead to elucidation of CRC pa...

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Autores principales:	Qingfei Pan, Xiaomin Lou, Ju Zhang, Yinghui Zhu, Fuqiang Li, Qiang Shan, Xianwei Chen, Yingying Xie, Siyuan Su, Hanfu Wei, Liang Lin, Lin Wu, Siqi Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f86b27beca4d48a7b6030cb5ccdfd5d0
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spelling	oai:doaj.org-article:f86b27beca4d48a7b6030cb5ccdfd5d02021-12-02T12:32:50ZGenomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer10.1038/s41598-017-00057-32045-2322https://doaj.org/article/f86b27beca4d48a7b6030cb5ccdfd5d02017-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00057-3https://doaj.org/toc/2045-2322Abstract Mouse model induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) is generally accepted as an ideal object to study on the carcinogenesis mechanisms of human colorectal cancer (CRC). The genomic responses to the AOM/DSS treatment in mouse that possibly lead to elucidation of CRC pathological mechanism are still poorly understood. For the first time, we investigated the cancer genome landscape of AOM/DSS mouse model by exome sequencing, to testify its molecular faithfulness to human CRC. Of 14 neoplastic samples, 7575 somatic variants were identified, which resulted in 2507 mutant genes and exhibited a large diversity in both colorectal aberrant crypt foci (ACF) and tumors even those tissues that were gained from the similar morphology or same treatment period. Cross-species comparison of the somatic variants demonstrated the totally different patterns of variable sites, mutant genes and perturbed pathways between mouse and human CRC. We therefore come to a conclusion that the tumorigenesis at genomic level in AOM/DSS model may not be properly comparable with that in human CRC, and the molecular mechanism elicited from this animal model should be carefully evaluated.Qingfei PanXiaomin LouJu ZhangYinghui ZhuFuqiang LiQiang ShanXianwei ChenYingying XieSiyuan SuHanfu WeiLiang LinLin WuSiqi LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingfei Pan Xiaomin Lou Ju Zhang Yinghui Zhu Fuqiang Li Qiang Shan Xianwei Chen Yingying Xie Siyuan Su Hanfu Wei Liang Lin Lin Wu Siqi Liu Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
description	Abstract Mouse model induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) is generally accepted as an ideal object to study on the carcinogenesis mechanisms of human colorectal cancer (CRC). The genomic responses to the AOM/DSS treatment in mouse that possibly lead to elucidation of CRC pathological mechanism are still poorly understood. For the first time, we investigated the cancer genome landscape of AOM/DSS mouse model by exome sequencing, to testify its molecular faithfulness to human CRC. Of 14 neoplastic samples, 7575 somatic variants were identified, which resulted in 2507 mutant genes and exhibited a large diversity in both colorectal aberrant crypt foci (ACF) and tumors even those tissues that were gained from the similar morphology or same treatment period. Cross-species comparison of the somatic variants demonstrated the totally different patterns of variable sites, mutant genes and perturbed pathways between mouse and human CRC. We therefore come to a conclusion that the tumorigenesis at genomic level in AOM/DSS model may not be properly comparable with that in human CRC, and the molecular mechanism elicited from this animal model should be carefully evaluated.
format	article
author	Qingfei Pan Xiaomin Lou Ju Zhang Yinghui Zhu Fuqiang Li Qiang Shan Xianwei Chen Yingying Xie Siyuan Su Hanfu Wei Liang Lin Lin Wu Siqi Liu
author_facet	Qingfei Pan Xiaomin Lou Ju Zhang Yinghui Zhu Fuqiang Li Qiang Shan Xianwei Chen Yingying Xie Siyuan Su Hanfu Wei Liang Lin Lin Wu Siqi Liu
author_sort	Qingfei Pan
title	Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
title_short	Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
title_full	Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
title_fullStr	Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
title_full_unstemmed	Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
title_sort	genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f86b27beca4d48a7b6030cb5ccdfd5d0
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Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer

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