Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates

DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined. However, their ability to relax supercoiling and unlink the pre-c...

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Bibliographic Details
Main Authors: Jorge Cebrián, Victor Martínez, Pablo Hernández, Dora B. Krimer, María-José Fernández-Nestosa, Jorge B. Schvartzman
Format: article
Language:EN
Published: MDPI AG 2021
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Online Access:https://doaj.org/article/f86df036de0b4fda942c8af1ffc489bc
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