Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis

Abstract Birdshot Retinochoroiditis (BSRC) is a progressive non-infectious intraocular inflammation that affects choroid and retina. Inflammatory processes have adverse effects on vision by affecting photoreceptor-bearing cells that do not regenerate. This study aimed at characterizing inflammatory...

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Autores principales:	Janine Trombke, Lucie Loyal, Julian Braun, Uwe Pleyer, Andreas Thiel, Dominika Pohlmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f878be41c869425e8cc85aeabcac2a0d
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spelling	oai:doaj.org-article:f878be41c869425e8cc85aeabcac2a0d2021-12-02T17:32:57ZAnalysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis10.1038/s41598-021-88013-02045-2322https://doaj.org/article/f878be41c869425e8cc85aeabcac2a0d2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88013-0https://doaj.org/toc/2045-2322Abstract Birdshot Retinochoroiditis (BSRC) is a progressive non-infectious intraocular inflammation that affects choroid and retina. Inflammatory processes have adverse effects on vision by affecting photoreceptor-bearing cells that do not regenerate. This study aimed at characterizing inflammatory CD4+ and CD8+ T cell subsets in the peripheral blood of active and inactive BSRCs. Furthermore, we correlated phenotypical and functional immunological analyses with clinical data. We observed a slight increase of terminally differentiated effector memory CD8+ T cells expressing CD45RA (TEMRA) in blood of inactive, compared to active BSRCs. Moreover, we identified a trend for a decreased population of TH2 cells and increased TH1 frequencies in active BSRCs, a typical sign of ongoing autoimmune processes. Functional assays demonstrated severe and overall impairment of effector function of both, CD4+ and CD8+ inflammatory T cells, which might reflect T cell exhaustion. Although the eye is the main site of inflammation in BSRC, we observed altered T cell subset compositions in the peripheral blood, dependent on the disease status. Our results indicate that T cells may play a major role in BSRC pathology, although our cohort size is too limited for definitve conclusions. Future studies with larger BSRCs have to be performed.Janine TrombkeLucie LoyalJulian BraunUwe PleyerAndreas ThielDominika PohlmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janine Trombke Lucie Loyal Julian Braun Uwe Pleyer Andreas Thiel Dominika Pohlmann Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
description	Abstract Birdshot Retinochoroiditis (BSRC) is a progressive non-infectious intraocular inflammation that affects choroid and retina. Inflammatory processes have adverse effects on vision by affecting photoreceptor-bearing cells that do not regenerate. This study aimed at characterizing inflammatory CD4+ and CD8+ T cell subsets in the peripheral blood of active and inactive BSRCs. Furthermore, we correlated phenotypical and functional immunological analyses with clinical data. We observed a slight increase of terminally differentiated effector memory CD8+ T cells expressing CD45RA (TEMRA) in blood of inactive, compared to active BSRCs. Moreover, we identified a trend for a decreased population of TH2 cells and increased TH1 frequencies in active BSRCs, a typical sign of ongoing autoimmune processes. Functional assays demonstrated severe and overall impairment of effector function of both, CD4+ and CD8+ inflammatory T cells, which might reflect T cell exhaustion. Although the eye is the main site of inflammation in BSRC, we observed altered T cell subset compositions in the peripheral blood, dependent on the disease status. Our results indicate that T cells may play a major role in BSRC pathology, although our cohort size is too limited for definitve conclusions. Future studies with larger BSRCs have to be performed.
format	article
author	Janine Trombke Lucie Loyal Julian Braun Uwe Pleyer Andreas Thiel Dominika Pohlmann
author_facet	Janine Trombke Lucie Loyal Julian Braun Uwe Pleyer Andreas Thiel Dominika Pohlmann
author_sort	Janine Trombke
title	Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
title_short	Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
title_full	Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
title_fullStr	Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
title_full_unstemmed	Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis
title_sort	analysis of peripheral inflammatory t cell subsets and their effector function in patients with birdshot retinochoroiditis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f878be41c869425e8cc85aeabcac2a0d
work_keys_str_mv	AT janinetrombke analysisofperipheralinflammatorytcellsubsetsandtheireffectorfunctioninpatientswithbirdshotretinochoroiditis AT lucieloyal analysisofperipheralinflammatorytcellsubsetsandtheireffectorfunctioninpatientswithbirdshotretinochoroiditis AT julianbraun analysisofperipheralinflammatorytcellsubsetsandtheireffectorfunctioninpatientswithbirdshotretinochoroiditis AT uwepleyer analysisofperipheralinflammatorytcellsubsetsandtheireffectorfunctioninpatientswithbirdshotretinochoroiditis AT andreasthiel analysisofperipheralinflammatorytcellsubsetsandtheireffectorfunctioninpatientswithbirdshotretinochoroiditis AT dominikapohlmann analysisofperipheralinflammatorytcellsubsetsandtheireffectorfunctioninpatientswithbirdshotretinochoroiditis
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Analysis of peripheral inflammatory T cell subsets and their effector function in patients with Birdshot Retinochoroiditis

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