Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range

Abstract Diet of the crab-eating monkey (Macaca fascicularis) consists of both plants and animals, including chitin-containing organisms such as crabs and insects. This omnivorous monkey has a high expression of acidic chitinase (CHIA) in the stomach and here, we report on its enzymatic properties u...

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Autores principales:	Maiko Uehara, Eri Tabata, Mikoto Okuda, Yukari Maruyama, Vaclav Matoska, Peter O. Bauer, Fumitaka Oyama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f87e4f5baa2d4a98bf1fb882837ec934
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spelling	oai:doaj.org-article:f87e4f5baa2d4a98bf1fb882837ec9342021-12-02T18:47:03ZRobust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range10.1038/s41598-021-95010-w2045-2322https://doaj.org/article/f87e4f5baa2d4a98bf1fb882837ec9342021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95010-whttps://doaj.org/toc/2045-2322Abstract Diet of the crab-eating monkey (Macaca fascicularis) consists of both plants and animals, including chitin-containing organisms such as crabs and insects. This omnivorous monkey has a high expression of acidic chitinase (CHIA) in the stomach and here, we report on its enzymatic properties under different conditions. When we compared with Mus musculus CHIA (Mm-CHIA), Macaca fascicularis CHIA (Mf-CHIA) exhibits higher chitinolytic activity at broad pH (1.0–7.0) and temperature (30–70 ℃) range. Interestingly, at its optimum pH (5.0), Mf-CHIA showed the highest activity at 65 °C while maintaining it at robust levels between 50 and 70 °C. The degradation efficiency of Mf-CHIA was superior to Mm-CHIA toward both polymeric chitin as well as an artificial chromogenic substrate. Our results show that unique features of Mf-CHIA including its thermostability warrant the nomination of this enzyme for potential agricultural and biomedical applications.Maiko UeharaEri TabataMikoto OkudaYukari MaruyamaVaclav MatoskaPeter O. BauerFumitaka OyamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maiko Uehara Eri Tabata Mikoto Okuda Yukari Maruyama Vaclav Matoska Peter O. Bauer Fumitaka Oyama Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range
description	Abstract Diet of the crab-eating monkey (Macaca fascicularis) consists of both plants and animals, including chitin-containing organisms such as crabs and insects. This omnivorous monkey has a high expression of acidic chitinase (CHIA) in the stomach and here, we report on its enzymatic properties under different conditions. When we compared with Mus musculus CHIA (Mm-CHIA), Macaca fascicularis CHIA (Mf-CHIA) exhibits higher chitinolytic activity at broad pH (1.0–7.0) and temperature (30–70 ℃) range. Interestingly, at its optimum pH (5.0), Mf-CHIA showed the highest activity at 65 °C while maintaining it at robust levels between 50 and 70 °C. The degradation efficiency of Mf-CHIA was superior to Mm-CHIA toward both polymeric chitin as well as an artificial chromogenic substrate. Our results show that unique features of Mf-CHIA including its thermostability warrant the nomination of this enzyme for potential agricultural and biomedical applications.
format	article
author	Maiko Uehara Eri Tabata Mikoto Okuda Yukari Maruyama Vaclav Matoska Peter O. Bauer Fumitaka Oyama
author_facet	Maiko Uehara Eri Tabata Mikoto Okuda Yukari Maruyama Vaclav Matoska Peter O. Bauer Fumitaka Oyama
author_sort	Maiko Uehara
title	Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range
title_short	Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range
title_full	Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range
title_fullStr	Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range
title_full_unstemmed	Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range
title_sort	robust chitinolytic activity of crab-eating monkey (macaca fascicularis) acidic chitinase under a broad ph and temperature range
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f87e4f5baa2d4a98bf1fb882837ec934
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Robust chitinolytic activity of crab-eating monkey (Macaca fascicularis) acidic chitinase under a broad pH and temperature range

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