Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species

Abstract Arginase (ARG) contributes to nitrogen remobilization by conversion of arginine to ornithine and urea. However, wheat ARG genes have not yet been identified. Here we isolated and characterized ARG genes from wheat and its progenitor species and found that a single copy was present in wheat...

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Autores principales:	Maoyun She, Jing Wang, Xinmin Wang, Guixiang Yin, Ke Wang, Lipu Du, Xingguo Ye
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f880b9d43b7c4c0eac3c76d4c76a747c
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spelling	oai:doaj.org-article:f880b9d43b7c4c0eac3c76d4c76a747c2021-12-02T11:40:52ZComprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species10.1038/s41598-017-07084-02045-2322https://doaj.org/article/f880b9d43b7c4c0eac3c76d4c76a747c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07084-0https://doaj.org/toc/2045-2322Abstract Arginase (ARG) contributes to nitrogen remobilization by conversion of arginine to ornithine and urea. However, wheat ARG genes have not yet been identified. Here we isolated and characterized ARG genes from wheat and its progenitor species and found that a single copy was present in wheat progenitors. Three common wheat ARG genes of TaARG-2AS, TaARG-2BS, and TaARG-2DS were experimentally assigned to the short arms of the group 2 chromosomes. We found an in-frame stop codon in TaARG-2AS, but not in the other two genes. The highest expression was detected in stems and sheaths for TaARG-2BS and in leaves for TaARG-2DS. Both genes have similar expression trend in different developmental stages, peaking at booting and grain filling stages. TaARG-2BS transcript was induced by high salinity and drought, whereas TaARG-2DS was induced by drought only, but neither of them were induced by low temperature. In addition, both genes showed analogous expression pattern upon powdery mildew (PM) infection in the resistant line Pm97033, with TaARG-2BS induced greatly at 72 h post PM infection. In contrast, no obvious transcripts were accumulated for TaARG-2DS in the PM susceptible line Wan7107. Monocot ARGs have more conserved mitochondrion-targeting signals and are more evolutionarily conserved than dicot ARGs.Maoyun SheJing WangXinmin WangGuixiang YinKe WangLipu DuXingguo YeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maoyun She Jing Wang Xinmin Wang Guixiang Yin Ke Wang Lipu Du Xingguo Ye Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
description	Abstract Arginase (ARG) contributes to nitrogen remobilization by conversion of arginine to ornithine and urea. However, wheat ARG genes have not yet been identified. Here we isolated and characterized ARG genes from wheat and its progenitor species and found that a single copy was present in wheat progenitors. Three common wheat ARG genes of TaARG-2AS, TaARG-2BS, and TaARG-2DS were experimentally assigned to the short arms of the group 2 chromosomes. We found an in-frame stop codon in TaARG-2AS, but not in the other two genes. The highest expression was detected in stems and sheaths for TaARG-2BS and in leaves for TaARG-2DS. Both genes have similar expression trend in different developmental stages, peaking at booting and grain filling stages. TaARG-2BS transcript was induced by high salinity and drought, whereas TaARG-2DS was induced by drought only, but neither of them were induced by low temperature. In addition, both genes showed analogous expression pattern upon powdery mildew (PM) infection in the resistant line Pm97033, with TaARG-2BS induced greatly at 72 h post PM infection. In contrast, no obvious transcripts were accumulated for TaARG-2DS in the PM susceptible line Wan7107. Monocot ARGs have more conserved mitochondrion-targeting signals and are more evolutionarily conserved than dicot ARGs.
format	article
author	Maoyun She Jing Wang Xinmin Wang Guixiang Yin Ke Wang Lipu Du Xingguo Ye
author_facet	Maoyun She Jing Wang Xinmin Wang Guixiang Yin Ke Wang Lipu Du Xingguo Ye
author_sort	Maoyun She
title	Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
title_short	Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
title_full	Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
title_fullStr	Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
title_full_unstemmed	Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
title_sort	comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f880b9d43b7c4c0eac3c76d4c76a747c
work_keys_str_mv	AT maoyunshe comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies AT jingwang comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies AT xinminwang comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies AT guixiangyin comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies AT kewang comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies AT lipudu comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies AT xingguoye comprehensivemolecularanalysisofarginaseencodinggenesincommonwheatanditsprogenitorspecies
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Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species

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