Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.

Scum is formed by the adsorption of long-chain fatty acids (LCFAs) onto biomass surface in anaerobic digestion of oily substrates. Since scum is a recalcitrant substrate to be digested, it is disposed via landfilling or incineration, which results in biomass washout and a decrease in methane yield....

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Autores principales:	Riku Sakurai, Shuhei Takizawa, Yasuhiro Fukuda, Chika Tada
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8849b91f72e49e1801a6bff37e41cea
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spelling	oai:doaj.org-article:f8849b91f72e49e1801a6bff37e41cea2021-12-02T20:13:58ZExploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.1932-620310.1371/journal.pone.0257651https://doaj.org/article/f8849b91f72e49e1801a6bff37e41cea2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257651https://doaj.org/toc/1932-6203Scum is formed by the adsorption of long-chain fatty acids (LCFAs) onto biomass surface in anaerobic digestion of oily substrates. Since scum is a recalcitrant substrate to be digested, it is disposed via landfilling or incineration, which results in biomass washout and a decrease in methane yield. The microbes contributing to scum degradation are unclear. This study aimed to investigate the cardinal microorganisms in anaerobic scum digestion. We pre-incubated a sludge with scum to enrich scum-degrading microbes. Using this sludge, a 1.3-times higher methane conversion rate (73%) and a faster LCFA degradation compared with control sludge were attained. Then, we analyzed the cardinal scum-degrading microbes in this pre-incubated sludge by changing the initial scum-loading rates. Increased 16S rRNA copy numbers for the syntrophic fatty-acid degrader Syntrophomonas and hydrogenotrophic methanogens were observed in scum high-loaded samples. 16S rRNA amplicon sequencing indicated that Syntrophomonas was the most abundant genus in all the samples. The amino-acid degrader Aminobacterium and hydrolytic genera such as Defluviitoga and Sporanaerobacter became more dominant as the scum-loading rate increased. Moreover, phylogenic analysis on Syntrophomonas revealed that Syntrophomonas palmitatica, which is capable of degrading LCFAs, related species became more dominant as the scum-loading rate increased. These results indicate that a variety of microorganisms that degrade LCFAs, proteins, and sugars are involved in effective scum degradation.Riku SakuraiShuhei TakizawaYasuhiro FukudaChika TadaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257651 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Riku Sakurai Shuhei Takizawa Yasuhiro Fukuda Chika Tada Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
description	Scum is formed by the adsorption of long-chain fatty acids (LCFAs) onto biomass surface in anaerobic digestion of oily substrates. Since scum is a recalcitrant substrate to be digested, it is disposed via landfilling or incineration, which results in biomass washout and a decrease in methane yield. The microbes contributing to scum degradation are unclear. This study aimed to investigate the cardinal microorganisms in anaerobic scum digestion. We pre-incubated a sludge with scum to enrich scum-degrading microbes. Using this sludge, a 1.3-times higher methane conversion rate (73%) and a faster LCFA degradation compared with control sludge were attained. Then, we analyzed the cardinal scum-degrading microbes in this pre-incubated sludge by changing the initial scum-loading rates. Increased 16S rRNA copy numbers for the syntrophic fatty-acid degrader Syntrophomonas and hydrogenotrophic methanogens were observed in scum high-loaded samples. 16S rRNA amplicon sequencing indicated that Syntrophomonas was the most abundant genus in all the samples. The amino-acid degrader Aminobacterium and hydrolytic genera such as Defluviitoga and Sporanaerobacter became more dominant as the scum-loading rate increased. Moreover, phylogenic analysis on Syntrophomonas revealed that Syntrophomonas palmitatica, which is capable of degrading LCFAs, related species became more dominant as the scum-loading rate increased. These results indicate that a variety of microorganisms that degrade LCFAs, proteins, and sugars are involved in effective scum degradation.
format	article
author	Riku Sakurai Shuhei Takizawa Yasuhiro Fukuda Chika Tada
author_facet	Riku Sakurai Shuhei Takizawa Yasuhiro Fukuda Chika Tada
author_sort	Riku Sakurai
title	Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
title_short	Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
title_full	Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
title_fullStr	Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
title_full_unstemmed	Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
title_sort	exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f8849b91f72e49e1801a6bff37e41cea
work_keys_str_mv	AT rikusakurai explorationofmicrobialcommunitiescontributingtoeffectivemethaneproductionfromscumunderanaerobicdigestion AT shuheitakizawa explorationofmicrobialcommunitiescontributingtoeffectivemethaneproductionfromscumunderanaerobicdigestion AT yasuhirofukuda explorationofmicrobialcommunitiescontributingtoeffectivemethaneproductionfromscumunderanaerobicdigestion AT chikatada explorationofmicrobialcommunitiescontributingtoeffectivemethaneproductionfromscumunderanaerobicdigestion
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Exploration of microbial communities contributing to effective methane production from scum under anaerobic digestion.

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