Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers

The presence of Staphylococcus spp. has increasingly been reported in food products and poses a public health threat. The aim of this study was to determine the diversity of Staphylococcus spp. and the antibiotic resistance profiles of isolates obtained from freshly harvested and packed ready-to-ea...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Stacey Duvenage, Werner Rossouw, Germán Villamizar-Rodríguez, Erika M. du Plessis, Lise Korsten
Formato:	article
Lenguaje:	EN
Publicado:	Academy of Science of South Africa 2021
Materias:	staphylococci antibiotic genes antibiotic resistance Science Q Science (General) Q1-390 Social Sciences H Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/f884e7fde60c4fc9a7460e3b060775f8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f884e7fde60c4fc9a7460e3b060775f8
record_format	dspace
spelling	oai:doaj.org-article:f884e7fde60c4fc9a7460e3b060775f82021-12-02T06:15:05ZAntibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers10.17159/sajs.2021/86671996-7489https://doaj.org/article/f884e7fde60c4fc9a7460e3b060775f82021-11-01T00:00:00Zhttps://sajs.co.za/article/view/8667https://doaj.org/toc/1996-7489 The presence of Staphylococcus spp. has increasingly been reported in food products and poses a public health threat. The aim of this study was to determine the diversity of Staphylococcus spp. and the antibiotic resistance profiles of isolates obtained from freshly harvested and packed ready-to-eat mushrooms (n=432) and handlers’ hands (n=150). A total of 56 Staphylococcus isolates [46.4% (n=26) from hands and 53.6% (n=30) from mushrooms] were recovered belonging to 10 species. Staphylococcus succinus isolates (n=21) were the most prevalent, of which 52.4% came from mushrooms and 47.6% from hands. This was followed by S. equorum isolates [n=12; 91.7% (n=11) from mushrooms and 8.3% (n=1) from hands] and S. saprophyticus [n=9; 66.7% (n=6) from mushrooms and 33.3% (n=3) from hands]. Six isolates that were characterised as multidrug resistant were isolated from hands of handlers. Most (83.9%; n=47) of the 56 isolates were resistant to penicillin [53.2% (n=25) from mushrooms and 46.8% (n=22) from hands] and 14.3% (n=8) were resistant to cephalosporin classes [25% (n=2) from mushrooms and 75% (n=6) from hands], both of which are used to treat staphylococcal infections. Antibiotic resistance genes blaZ [25.0% (n=14) of all isolates of which 71.4% (n=10) were from hands and 28.57% (n=4) from mushrooms], tetL and tetK [both 1.8% (n=1) from hands], mecA [5.4% (n=3) from hands] and ermA [1.8% (n=1) from mushrooms] were detected from the 56 isolates. Only two (25.0%) of the eight methicillin-resistant staphylococci harboured the mecA gene, while only 11 (23%) of the 47 penicillin-resistant isolates harboured the blaZ gene [36.4% (n=4) from mushrooms and 63.6% (n=7) from hands]. Our results demonstrate that food handlers and harvested and packed ready-to-eat mushrooms could be a source of diverse Staphylococcus spp. that exhibit antimicrobial resistance. Clinically relevant S. aureus was only detected on one handler’s hand; however, the isolate was not multidrug resistant. The presence of diverse Staphylococcus spp. on mushrooms and the hands of handlers is a potential public health concern due to their potential to cause opportunistic infections. Significance: • This study is the first to describe the antibiotic resistance profiles and antibiotic gene presence of Staphylococcus spp. isolated from fresh mushrooms and hands of pickers and packers. Mushrooms and handlers in this study were demonstrated to be possible routes of transmission of Staphylococcus spp. that are antibiotic resistant and which harbour antibiotic resistance genes, presenting a possible public health hazard. Stacey DuvenageWerner RossouwGermán Villamizar-RodríguezErika M. du PlessisLise KorstenAcademy of Science of South Africaarticlestaphylococciantibiotic genesantibiotic resistanceScienceQScience (General)Q1-390Social SciencesHSocial sciences (General)H1-99ENSouth African Journal of Science, Vol 117, Iss 11/12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	staphylococci antibiotic genes antibiotic resistance Science Q Science (General) Q1-390 Social Sciences H Social sciences (General) H1-99
spellingShingle	staphylococci antibiotic genes antibiotic resistance Science Q Science (General) Q1-390 Social Sciences H Social sciences (General) H1-99 Stacey Duvenage Werner Rossouw Germán Villamizar-Rodríguez Erika M. du Plessis Lise Korsten Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers
description	The presence of Staphylococcus spp. has increasingly been reported in food products and poses a public health threat. The aim of this study was to determine the diversity of Staphylococcus spp. and the antibiotic resistance profiles of isolates obtained from freshly harvested and packed ready-to-eat mushrooms (n=432) and handlers’ hands (n=150). A total of 56 Staphylococcus isolates [46.4% (n=26) from hands and 53.6% (n=30) from mushrooms] were recovered belonging to 10 species. Staphylococcus succinus isolates (n=21) were the most prevalent, of which 52.4% came from mushrooms and 47.6% from hands. This was followed by S. equorum isolates [n=12; 91.7% (n=11) from mushrooms and 8.3% (n=1) from hands] and S. saprophyticus [n=9; 66.7% (n=6) from mushrooms and 33.3% (n=3) from hands]. Six isolates that were characterised as multidrug resistant were isolated from hands of handlers. Most (83.9%; n=47) of the 56 isolates were resistant to penicillin [53.2% (n=25) from mushrooms and 46.8% (n=22) from hands] and 14.3% (n=8) were resistant to cephalosporin classes [25% (n=2) from mushrooms and 75% (n=6) from hands], both of which are used to treat staphylococcal infections. Antibiotic resistance genes blaZ [25.0% (n=14) of all isolates of which 71.4% (n=10) were from hands and 28.57% (n=4) from mushrooms], tetL and tetK [both 1.8% (n=1) from hands], mecA [5.4% (n=3) from hands] and ermA [1.8% (n=1) from mushrooms] were detected from the 56 isolates. Only two (25.0%) of the eight methicillin-resistant staphylococci harboured the mecA gene, while only 11 (23%) of the 47 penicillin-resistant isolates harboured the blaZ gene [36.4% (n=4) from mushrooms and 63.6% (n=7) from hands]. Our results demonstrate that food handlers and harvested and packed ready-to-eat mushrooms could be a source of diverse Staphylococcus spp. that exhibit antimicrobial resistance. Clinically relevant S. aureus was only detected on one handler’s hand; however, the isolate was not multidrug resistant. The presence of diverse Staphylococcus spp. on mushrooms and the hands of handlers is a potential public health concern due to their potential to cause opportunistic infections. Significance: • This study is the first to describe the antibiotic resistance profiles and antibiotic gene presence of Staphylococcus spp. isolated from fresh mushrooms and hands of pickers and packers. Mushrooms and handlers in this study were demonstrated to be possible routes of transmission of Staphylococcus spp. that are antibiotic resistant and which harbour antibiotic resistance genes, presenting a possible public health hazard.
format	article
author	Stacey Duvenage Werner Rossouw Germán Villamizar-Rodríguez Erika M. du Plessis Lise Korsten
author_facet	Stacey Duvenage Werner Rossouw Germán Villamizar-Rodríguez Erika M. du Plessis Lise Korsten
author_sort	Stacey Duvenage
title	Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers
title_short	Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers
title_full	Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers
title_fullStr	Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers
title_full_unstemmed	Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers
title_sort	antibiotic resistance profiles of staphylococcus spp. from white button mushrooms and handlers
publisher	Academy of Science of South Africa
publishDate	2021
url	https://doaj.org/article/f884e7fde60c4fc9a7460e3b060775f8
work_keys_str_mv	AT staceyduvenage antibioticresistanceprofilesofstaphylococcussppfromwhitebuttonmushroomsandhandlers AT wernerrossouw antibioticresistanceprofilesofstaphylococcussppfromwhitebuttonmushroomsandhandlers AT germanvillamizarrodriguez antibioticresistanceprofilesofstaphylococcussppfromwhitebuttonmushroomsandhandlers AT erikamduplessis antibioticresistanceprofilesofstaphylococcussppfromwhitebuttonmushroomsandhandlers AT lisekorsten antibioticresistanceprofilesofstaphylococcussppfromwhitebuttonmushroomsandhandlers
_version_	1718400029954146304

Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers

Ejemplares similares