Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice

Heejeong Hong,1 Hyun-Kuk Song,1 Bohee Jang,1 Eunhye Park,1 Dong Soo Han,2 Seong-Eun Kim,3 Eok-Soo Oh1 1Department of Life Sciences, Ewha Womans University, Seoul, South Korea; 2Department of Internal Medicine, Hanyang University College of Medicine, Guri, South Korea; 3Department of Internal Medicin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hong H, Song HK, Jang B, Park E, Han DS, Kim SE, Oh ES
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	chronic inflammation ∙ syndecan-2 ∙ mmp-7 ∙ shedding Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/f88527d98f7e4a6fad73cc712e6287e7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f88527d98f7e4a6fad73cc712e6287e7
record_format	dspace
spelling	oai:doaj.org-article:f88527d98f7e4a6fad73cc712e6287e72021-12-02T19:17:11ZColocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice1178-7031https://doaj.org/article/f88527d98f7e4a6fad73cc712e6287e72021-09-01T00:00:00Zhttps://www.dovepress.com/colocalization-with-mmp-7-in-the-distal-colon-is-crucial-for-syndecan--peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Heejeong Hong,1 Hyun-Kuk Song,1 Bohee Jang,1 Eunhye Park,1 Dong Soo Han,2 Seong-Eun Kim,3 Eok-Soo Oh1 1Department of Life Sciences, Ewha Womans University, Seoul, South Korea; 2Department of Internal Medicine, Hanyang University College of Medicine, Guri, South Korea; 3Department of Internal Medicine, Ewha Womans University School of Medicine, Ewha Medical Research Institute, Seoul, South KoreaCorrespondence: Eok-Soo OhDepartment of Life Sciences, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemoon-Gu, Seoul, 03760, South KoreaTel +82-2-3277-3761Fax +82-2-3277-3760Email OhES@ewha.ac.krIntroduction: Syndecan-2 expression is elevated during chronic inflammation and cancer development, and its shedding is observed in cancer patients. However, it remained unknown whether inflammation triggers syndecan-2 shedding.Methods: The colitis model was produced in C57BL/6 mice by oral administration of 2– 3% dextran sulfate sodium (DSS) in the drinking water. Syndecan-2 and MMP-7 expression levels in tissues and cells were detected by real-time PCR, Western blotting, and immunohistochemistry. Shed syndecan-2 levels were detected by slot blotting. For tissue culture, colon tissues were divided into proximal, transverse, and distal parts, and incubated in culture media.Results: In C57BL/6 mice with DSS-induced colitis, syndecan-2 shedding began to increase after week 12 of chronic inflammation and continued to increase at week 15. The level of shed syndecan-2 correlated with the colocalization of syndecan-2 and MMP-7 in distal colon tissues. The mRNA expression of IL-6 was increased specifically in trans-distal colon tissues from weeks 9 to 15. IL-6 induced syndecan-2 expression and shedding and MMP-7 expression in ex vivo-cultured distal colon tissues and adenoma cell lines derived from the distal colon. IL-6 treatment induced STAT3 phosphorylation and MMP-7 expression in DLD-1 cells. The application of MMP-7 to ex vivo-cultured colon tissues increased the shedding of syndecan-2 to the culture medium.Conclusion: Our findings suggest that chronic inflammation induces syndecan-2 shedding via the site-specific colocalization of syndecan-2 with MMP-7 in the distal colon.Keywords: chronic inflammation, syndecan-2, MMP-7, sheddingHong HSong HKJang BPark EHan DSKim SEOh ESDove Medical Pressarticlechronic inflammation ∙ syndecan-2 ∙ mmp-7 ∙ sheddingPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 4987-5000 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic inflammation ∙ syndecan-2 ∙ mmp-7 ∙ shedding Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	chronic inflammation ∙ syndecan-2 ∙ mmp-7 ∙ shedding Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Hong H Song HK Jang B Park E Han DS Kim SE Oh ES Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
description	Heejeong Hong,1 Hyun-Kuk Song,1 Bohee Jang,1 Eunhye Park,1 Dong Soo Han,2 Seong-Eun Kim,3 Eok-Soo Oh1 1Department of Life Sciences, Ewha Womans University, Seoul, South Korea; 2Department of Internal Medicine, Hanyang University College of Medicine, Guri, South Korea; 3Department of Internal Medicine, Ewha Womans University School of Medicine, Ewha Medical Research Institute, Seoul, South KoreaCorrespondence: Eok-Soo OhDepartment of Life Sciences, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemoon-Gu, Seoul, 03760, South KoreaTel +82-2-3277-3761Fax +82-2-3277-3760Email OhES@ewha.ac.krIntroduction: Syndecan-2 expression is elevated during chronic inflammation and cancer development, and its shedding is observed in cancer patients. However, it remained unknown whether inflammation triggers syndecan-2 shedding.Methods: The colitis model was produced in C57BL/6 mice by oral administration of 2– 3% dextran sulfate sodium (DSS) in the drinking water. Syndecan-2 and MMP-7 expression levels in tissues and cells were detected by real-time PCR, Western blotting, and immunohistochemistry. Shed syndecan-2 levels were detected by slot blotting. For tissue culture, colon tissues were divided into proximal, transverse, and distal parts, and incubated in culture media.Results: In C57BL/6 mice with DSS-induced colitis, syndecan-2 shedding began to increase after week 12 of chronic inflammation and continued to increase at week 15. The level of shed syndecan-2 correlated with the colocalization of syndecan-2 and MMP-7 in distal colon tissues. The mRNA expression of IL-6 was increased specifically in trans-distal colon tissues from weeks 9 to 15. IL-6 induced syndecan-2 expression and shedding and MMP-7 expression in ex vivo-cultured distal colon tissues and adenoma cell lines derived from the distal colon. IL-6 treatment induced STAT3 phosphorylation and MMP-7 expression in DLD-1 cells. The application of MMP-7 to ex vivo-cultured colon tissues increased the shedding of syndecan-2 to the culture medium.Conclusion: Our findings suggest that chronic inflammation induces syndecan-2 shedding via the site-specific colocalization of syndecan-2 with MMP-7 in the distal colon.Keywords: chronic inflammation, syndecan-2, MMP-7, shedding
format	article
author	Hong H Song HK Jang B Park E Han DS Kim SE Oh ES
author_facet	Hong H Song HK Jang B Park E Han DS Kim SE Oh ES
author_sort	Hong H
title	Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
title_short	Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
title_full	Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
title_fullStr	Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
title_full_unstemmed	Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice
title_sort	colocalization with mmp-7 in the distal colon is crucial for syndecan-2 shedding in dextran sulfate sodium-induced colitis mice
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/f88527d98f7e4a6fad73cc712e6287e7
work_keys_str_mv	AT hongh colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice AT songhk colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice AT jangb colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice AT parke colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice AT hands colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice AT kimse colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice AT ohes colocalizationwithmmp7inthedistalcoloniscrucialforsyndecan2sheddingindextransulfatesodiuminducedcolitismice
_version_	1718376949985837056

Colocalization with MMP-7 in the Distal Colon is Crucial for Syndecan-2 Shedding in Dextran Sulfate Sodium-Induced Colitis Mice

Ejemplares similares