Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography

Abstract In this study, a medical device made of surface microstructured bacterial cellulose was produced using cellulose-producing acetic acid bacteria wild-type strains in combination with guided assembly-based biolithography. The medical device aims at interfering with the cell's focal adhes...

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Autores principales:	Marcello Brugnoli, Francesco Robotti, Salvatore La China, Kavitha Anguluri, Hossein Haghighi, Simone Bottan, Aldo Ferrari, Maria Gullo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8880a0f1d124acbaf472238ef2080a1
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spelling	oai:doaj.org-article:f8880a0f1d124acbaf472238ef2080a12021-12-02T18:51:28ZAssessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography10.1038/s41598-021-98705-22045-2322https://doaj.org/article/f8880a0f1d124acbaf472238ef2080a12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98705-2https://doaj.org/toc/2045-2322Abstract In this study, a medical device made of surface microstructured bacterial cellulose was produced using cellulose-producing acetic acid bacteria wild-type strains in combination with guided assembly-based biolithography. The medical device aims at interfering with the cell's focal adhesion establishment and maturation around implantable devices placed in soft tissues by the symmetrical array on its surface. A total of 25 Komagataeibacter strains was evaluated over a three-step selection. In the first step, the ability of strains to produce a suitable bacterial cellulose layer with high production yield was examined, then nine strains, with a uniform and smooth layer of bacterial cellulose, were cultured in a custom-made silicone bioreactor and finally the characteristics of the symmetrical array of topographic features on the surface were analysed. Selected strains showed high inter and intra species variability in bacterial cellulose production. The devices obtained by K2G30, K1G4, DSM 46590 (Komagataeibacter xylinus), K2A8 (Komagataeibacter sp.) and DSM 15973T (Komagataeibacter sucrofermentas) strains were pouched-formed with hexagonal surface pattern required for reducing the formation of fibrotic tissue around devices, once they are implanted in soft tissues. Our findings revealed the effectiveness of the selected Komagataeibacter wild-type strains in producing surface microstructured bacterial cellulose pouches for making biomedical devices.Marcello BrugnoliFrancesco RobottiSalvatore La ChinaKavitha AnguluriHossein HaghighiSimone BottanAldo FerrariMaria GulloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcello Brugnoli Francesco Robotti Salvatore La China Kavitha Anguluri Hossein Haghighi Simone Bottan Aldo Ferrari Maria Gullo Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
description	Abstract In this study, a medical device made of surface microstructured bacterial cellulose was produced using cellulose-producing acetic acid bacteria wild-type strains in combination with guided assembly-based biolithography. The medical device aims at interfering with the cell's focal adhesion establishment and maturation around implantable devices placed in soft tissues by the symmetrical array on its surface. A total of 25 Komagataeibacter strains was evaluated over a three-step selection. In the first step, the ability of strains to produce a suitable bacterial cellulose layer with high production yield was examined, then nine strains, with a uniform and smooth layer of bacterial cellulose, were cultured in a custom-made silicone bioreactor and finally the characteristics of the symmetrical array of topographic features on the surface were analysed. Selected strains showed high inter and intra species variability in bacterial cellulose production. The devices obtained by K2G30, K1G4, DSM 46590 (Komagataeibacter xylinus), K2A8 (Komagataeibacter sp.) and DSM 15973T (Komagataeibacter sucrofermentas) strains were pouched-formed with hexagonal surface pattern required for reducing the formation of fibrotic tissue around devices, once they are implanted in soft tissues. Our findings revealed the effectiveness of the selected Komagataeibacter wild-type strains in producing surface microstructured bacterial cellulose pouches for making biomedical devices.
format	article
author	Marcello Brugnoli Francesco Robotti Salvatore La China Kavitha Anguluri Hossein Haghighi Simone Bottan Aldo Ferrari Maria Gullo
author_facet	Marcello Brugnoli Francesco Robotti Salvatore La China Kavitha Anguluri Hossein Haghighi Simone Bottan Aldo Ferrari Maria Gullo
author_sort	Marcello Brugnoli
title	Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
title_short	Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
title_full	Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
title_fullStr	Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
title_full_unstemmed	Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
title_sort	assessing effectiveness of komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f8880a0f1d124acbaf472238ef2080a1
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Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography

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