Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas

Abstract. Devi AR, Susilowati A, Setyaningsih R. 2019. Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas 20: 2890-2896. Seafood is very popular among Indonesian people, especially in coas...

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Autores principales:	ANNA ROOSIANA DEVI, Ari Susilowati, RATNA SETYANINGSIH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	16s rrna, blood clams, molecular identification, morphology, molecular, vibrio Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f8888131cecc47c0bcfdf39aaa407161
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spelling	oai:doaj.org-article:f8888131cecc47c0bcfdf39aaa4071612021-11-21T21:54:58ZMorphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas1412-033X2085-472210.13057/biodiv/d201016https://doaj.org/article/f8888131cecc47c0bcfdf39aaa4071612019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4036https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Devi AR, Susilowati A, Setyaningsih R. 2019. Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas 20: 2890-2896. Seafood is very popular among Indonesian people, especially in coastal areas. In Bantul Yogyakarta, blood clams have become one of tourist's favorite, either cooked or raw. Blood clams are filter feeders that cause the clams to be vulnerable to contamination of pathogenic bacteria that can cause foodborne disease, including Escherichia, Pseudomonas, and Vibrio. The 10-20% cases of foodborne disease transmitted through seafood caused by Vibrio spp. Three species of Vibrio can cause foodborne disease in humans, i.e., V. cholerae, V. parahaemolyticus, and V. vulnificus. The purpose of this study was to determine the character of Vibrio using morphological and molecular identification and pathogenicity on blood clam (Anadara granosa). Blood clams samples were collected from Depok, Goa Cemara, and Kwaru beaches in Bantul, Yogyakarta, Indonesia. Isolation of Vibrio spp. from blood clams was done using selective differential Thiosulfate Citrate bile salts sucrose (TCBS) culture medium. The morphological characters of the isolate colonies were determined based on the color, shape, texture, and size of the colony. Hemolysis test was also performed to evaluate the pathogenicity by using blood agar media. Molecular identification of Vibrio species was made using 16S rRNA sequence. Phylogenetic analysis was performed using Neighbor-Joining method in Mega X software. Samples for the analysis came from DNA sequences of this study and those from the GenBank database. Of the total 15 isolates obtained, four isolates showed positive ?-hemolysis, namely, isolate P2S2 -1bH, P3S1-1aH, P2S1-1aK and P2S2-1aK, and one isolate had positive ?-hemolysis (P3S2-1aK). Seven species of Vibrio were identified as V. algynolyticus, V. parahaemolyticus, V. diabolicus, V neocaledonicus, V. azureus, V. natrigens, and V. cholerae.ANNA ROOSIANA DEVIAri SusilowatiRATNA SETYANINGSIHMBI & UNS Soloarticle16s rrna, blood clams, molecular identification, morphology, molecular, vibrioBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna, blood clams, molecular identification, morphology, molecular, vibrio Biology (General) QH301-705.5
spellingShingle	16s rrna, blood clams, molecular identification, morphology, molecular, vibrio Biology (General) QH301-705.5 ANNA ROOSIANA DEVI Ari Susilowati RATNA SETYANINGSIH Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas
description	Abstract. Devi AR, Susilowati A, Setyaningsih R. 2019. Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas 20: 2890-2896. Seafood is very popular among Indonesian people, especially in coastal areas. In Bantul Yogyakarta, blood clams have become one of tourist's favorite, either cooked or raw. Blood clams are filter feeders that cause the clams to be vulnerable to contamination of pathogenic bacteria that can cause foodborne disease, including Escherichia, Pseudomonas, and Vibrio. The 10-20% cases of foodborne disease transmitted through seafood caused by Vibrio spp. Three species of Vibrio can cause foodborne disease in humans, i.e., V. cholerae, V. parahaemolyticus, and V. vulnificus. The purpose of this study was to determine the character of Vibrio using morphological and molecular identification and pathogenicity on blood clam (Anadara granosa). Blood clams samples were collected from Depok, Goa Cemara, and Kwaru beaches in Bantul, Yogyakarta, Indonesia. Isolation of Vibrio spp. from blood clams was done using selective differential Thiosulfate Citrate bile salts sucrose (TCBS) culture medium. The morphological characters of the isolate colonies were determined based on the color, shape, texture, and size of the colony. Hemolysis test was also performed to evaluate the pathogenicity by using blood agar media. Molecular identification of Vibrio species was made using 16S rRNA sequence. Phylogenetic analysis was performed using Neighbor-Joining method in Mega X software. Samples for the analysis came from DNA sequences of this study and those from the GenBank database. Of the total 15 isolates obtained, four isolates showed positive ?-hemolysis, namely, isolate P2S2 -1bH, P3S1-1aH, P2S1-1aK and P2S2-1aK, and one isolate had positive ?-hemolysis (P3S2-1aK). Seven species of Vibrio were identified as V. algynolyticus, V. parahaemolyticus, V. diabolicus, V neocaledonicus, V. azureus, V. natrigens, and V. cholerae.
format	article
author	ANNA ROOSIANA DEVI Ari Susilowati RATNA SETYANINGSIH
author_facet	ANNA ROOSIANA DEVI Ari Susilowati RATNA SETYANINGSIH
author_sort	ANNA ROOSIANA DEVI
title	Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas
title_short	Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas
title_full	Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas
title_fullStr	Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas
title_full_unstemmed	Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas
title_sort	morphology, molecular identification, and pathogenicity of vibrio spp. on blood clam (anadara granosa) in yogyakarta, indonesia tourism beach areas
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/f8888131cecc47c0bcfdf39aaa407161
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Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas

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